A5075844386- Neuroscience and Neuropharmacology Research
- Science, Research, and Medicine
- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Receptor Mechanisms and Signaling
- Neurogenesis and neuroplasticity mechanisms
- Climate Change and Health Impacts
- Stress Responses and Cortisol
- Air Quality and Health Impacts
- Hormonal Regulation and Hypertension
- Neuropeptides and Animal Physiology
- Pharmacogenetics and Drug Metabolism
- Chemical Reactions and Isotopes
- RNA regulation and disease
- Parkinson's Disease Mechanisms and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- 3D Printing in Biomedical Research
- Attention Deficit Hyperactivity Disorder
- Diet and metabolism studies
- 14-3-3 protein interactions
- Pharmacological Receptor Mechanisms and Effects
- Birth, Development, and Health
- MicroRNA in disease regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Indoor Air Quality and Microbial Exposure
Polish Academy of Sciences
2018-2025
Maj Institute of Pharmacology
2018-2025
L-3,4-Dihydroxyphenylalanine (L-DOPA) is effective at alleviating motor impairments in Parkinson's disease (PD) patients but has mixed effects on nonmotor symptoms and causes adverse after prolonged treatment. Here, we analyzed the spatial profile of L-DOPA-induced gene expression forebrain mice with an inducible progressive loss dopaminergic neurons (the TIF-IADATCreERT2 strain), a focus similarities differences areas relevant to different PD symptoms. The animals received 14-day L-DOPA...
Abstract Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration and neuropsychiatric abnormalities. Loss of full-length dystrophins both necessary sufficient to initiate DMD. These isoforms are expressed in the hippocampus, cerebral cortex (Dp427c), cerebellar Purkinje cells (Dp427p). However, our understanding consequences their absence, which crucial for developing targeted interventions, remains inadequate. We combined RNA sequencing with genome-scale...
<title>Abstract</title> Glucocorticoids, acting through the glucocorticoid receptor (GR), control metabolism, maintain homeostasis, and enable adaptive responses to environmental challenges. Their function has been comprehensively studied, leading identification of numerous tissue-specific GR-dependent mechanisms. Abundant evidence shows that GR-triggered differ across tissues, however, extent this specificity was not explored. It is also unknown how particular GR-induced molecular patterns...
Mutations in the DMD gene, encoding dystrophins, cause progressive muscle degeneration with severe sterile inflammation. While macrophages predominate amongst muscle-infiltrating cells, being central to both damage and regeneration, they were not known express dystrophin. Yet, we recently demonstrated Dp71 dystrophin expression correlating tumour infiltrating macrophages. Here report physiological, developmentally regulated of human mouse hematopoietic stem which decreases cell maturation...
Genetic variants affect baseline health and biomarker values, which in turn impact both the therapy selected for an individual magnitude of change induced by medication. Here, we propose approach complex longitudinal repeated measures biobank data, separates genetic effects disease from medication usage those treatment response. For 211,845 individuals, construct a pre-post study design 1,420,443 blood pressure (BP) measurements 1,117,900 prescription records common BP influencing drugs,...
Three strains of mice with various susceptibilities to restraint stress (RS), i.e., a knocked out norepinephrine transporter gene (NET-KO), SWR/J and C57BL/6J (WT) were shown serve as good model study the molecular mechanisms underlying different stress-coping strategies. We identified 14 miRNAs that altered by RS in PFC these genotype-dependent manner, where most interesting was let-7e. Further silico analysis its potential targets allowed us identify five mRNAs (Bcl2l11, Foxo1, Pik3r1,...
Abstract Alterations in Dp71 expression, the most ubiquitous dystrophin isoform, have been associated with patient survival across tumours. Intriguingly, certain malignancies, acts as a tumour suppressor, while manifesting oncogenic properties others. This diversity could be explained by expression of two splice variants encoding proteins distinct C-termini, each specific properties. Expression these has impeded exploration their unique roles. Using CRISPR/Cas9, we ablated Dp71f variant...
Oprm1, the gene encoding μ-opioid receptor, has multiple reported transcripts, with a variable 3' region and many alternative sequences C-terminus of protein. The functional implications this variability remain mostly unexplored, though recurring notion is that it could be exploited by developing selective ligands improved clinical profiles. Here, we comprehensively examined Oprm1 transcriptional variants in murine central nervous system, using long-read RNAseq as well spatial single-cell...
The long-term effects of psychotropic drugs are associated with the reversal disease-related alterations through reorganization and normalization neuronal connections. Molecular factors that trigger drug-induced brain plasticity remain only partly understood. Doublecortin-like kinase 1 (Dclk1) possesses microtubule-polymerizing activity during synaptic neurogenesis. However, Dclk1 gene shows a complex profile transcriptional regulation, two alternative promoters exon splicing patterns...
Dopamine receptor D2 gene (DRD2) polymorphisms have been associated with cognitive abilities, obesity, addictions, and physical-activity-related behaviors, which may underlie differences in the effectiveness of training programs. What is not yet clear impact DRD2 on exercise Thus, aim this study was to investigate association between polymorphic sites (rs1076560, rs12364283, rs1799732, rs1800497, rs1800498) body's response regular physical activity. We studied genotypes haplotypes...
Despite the general awareness of need to reduce air pollution, efforts were undertaken in Poland eliminate pollutants and their harmful effect on human health seem be insufficient. Moreover, latest data indicate that city Krakow is at forefront most polluted cities worldwide. Hence, this report, we investigated impact particulate matter isolated from (PM KRK) gene expression profile peripheral blood mononuclear cells (PBMCs) healthy donors (HD) patients with atherosclerosis (AS), rheumatoid...
Abstract Background Oprm1 , the gene encoding μ-opioid receptor, has multiple reported transcripts, with a variable 3 region and many alternative sequences C-terminus of protein. The functional implications this variability remain mostly unexplored, though recurring notion is that it could be exploited by developing selective ligands improved clinical profiles. Here, we comprehensively examined transcriptional variants in murine central nervous system. Methods RNA-seq transcription analyses...
Prenatal excess of glucocorticoids (GCs) is considered to be one the highly impacting factors contributing depression development. Although GCs are crucial for normal fetal development and their administration (mainly dexamethasone, DEX) a life-saving procedure those at risk preterm delivery, exposure levels during pregnancy can yield detrimental consequences. Therefore, we aimed systematically investigate brain molecular alterations triggered by prenatal DEX administration. We used rat...
Abstract The acceptability of antidepressant drugs partly depends on genetic factors. list genes involved in response, including Adverse Drug Reactions (ADRs) is broad and contains both drug-metabolizing enzymes (pharmacogenes) pharmacodynamics. Variants pharmacogenes are traditionally reported the form star alleles partially annotated with known phenotypic consequences. As it unfeasible to analyze all genotype-phenotype pairs, computational approaches remain practical solution. A...
Despite the variable chemical and physical characteristics of particulate air pollutants, inflammation oxidative stress have been identified as common mechanisms for cell damage negative health influences. These effects are produced by organic components, especially endotoxins. This study analyzed gene expression profile after exposure RAW 264.7 cells to standard matter (PM) material, NIST1648a, PM with a reduced content, LAp120, in comparison lipopolysaccharide (LPS). The selected...