A5057515518- Trace Elements in Health
- Platelet Disorders and Treatments
- Chemical Synthesis and Analysis
- Antiplatelet Therapy and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- Amino Acid Enzymes and Metabolism
- Blood properties and coagulation
- Metal complexes synthesis and properties
- Computational Drug Discovery Methods
- Infective Endocarditis Diagnosis and Management
- RNA and protein synthesis mechanisms
- Connective tissue disorders research
- Proteoglycans and glycosaminoglycans research
- Streptococcal Infections and Treatments
- Collagen: Extraction and Characterization
- Iron Metabolism and Disorders
- Epigenetics and DNA Methylation
- Contact Dermatitis and Allergies
- Molecular Sensors and Ion Detection
- Alzheimer's disease research and treatments
- Heavy Metal Exposure and Toxicity
- Mass Spectrometry Techniques and Applications
- Skin and Cellular Biology Research
- RNA Research and Splicing
- Drug Transport and Resistance Mechanisms
University of Cambridge
2016-2025
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2010-2022
Lawn Tennis Association
2019
Polish Academy of Sciences
2010-2014
In-Q-Tel
2011
University of Gdańsk
2003-2008
Accumulation of the β-amyloid (Aβ) peptide in extracellular senile plaques rich copper and zinc is a defining pathological feature Alzheimer's disease (AD). The Aβ1-x (x=16/28/40/42) peptides have been primary focus Cu(II) binding studies for more than 15 years; however, N-truncated Aβ4-42 major Aβ isoform detected both healthy diseased brains, it contains novel N-terminal FRH sequence. Proteins with His at third position are known to bind avidly, conditional log K values pH 7.4 range...
Essentials•Glycoprotein VI (GPVI) binds collagen, starting thrombogenesis, and fibrin, stabilizing thrombi.•GPVI‐dimers, not monomers, recognize immobilized fibrinogen fibrin through their D‐domains.•Collagen, D‐fragment D‐dimer may share a common or proximate binding site(s) on GPVI‐dimer.•GPVI‐dimer–fibrin interaction supports spreading, activation adhesion involving αIIbβ3.Summary: BackgroundPlatelet collagen receptor Glycoprotein initiating stabilizes thrombi by fibrin.ObjectivesTo...
The hallmark of fibrotic disorders is a highly cross-linked and dense collagen matrix, property driven by the oxidative action lysyl oxidase. Other fibrosis-associated proteins also contribute to final matrix properties, one which fibromodulin. Its interactions with affect cross-linking, packing, fibril diameter. We investigated possibility that specific relationship exists between fibromodulin oxidase, potentially imparting phenotype. mapped fibromodulin-collagen interaction sites using II...
Fibrillar collagens of the extracellular matrix are critical for tissue structure and physiology; however, excessive or abnormal deposition is a defining feature fibrosis. Regulatory mechanisms that act on collagen fibril assembly potentially offer new targets antifibrotic treatments. Tissue weakening, altered morphologies, both, shared phenotypes mice lacking matricellular thrombospondins. Thrombospondin-1 (TSP1) plays an indirect role in homeostasis through interactions with...
Abstract Tissue factor (TF) plays a central role in haemostasis and thrombosis. Following vascular damage, vessel wall TF initiates the extrinsic coagulation cascade. can also be exposed by monocytes. Inflammatory or infectious stimuli trigger synthesis of new protein monocytes over course hours. It has been suggested that expose within minutes when stimulated activated platelets. Here, we have confirmed rapidly whole blood further demonstrate platelet P-selectin exposure is necessary...
The M protein is an essential virulence factor of Streptococcus pyogenes , or group A streptococci (GAS), one the most common and dangerous human pathogens. Molecular functional characterization variants their interactions with host components crucial for understanding streptococcal pathogenesis vaccine development. M3 produced by prevalent emm 3 GAS serotype, which frequently associated severe invasive diseases. Here we characterize interaction collagens through detailed structural...
The M protein is an essential virulence factor of Streptococcus pyogenes , or group A streptococci (GAS), one the most common and dangerous human pathogens. Molecular functional characterization variants their interactions with host components crucial for understanding streptococcal pathogenesis vaccine development. M3 produced by prevalent emm 3 GAS serotype, which frequently associated severe invasive diseases. Here we characterize interaction collagens through detailed structural...
Recently we screened a combinatorial library of R1-(Ser/Thr)-Xaa-His-Zaa-R2 peptides (Xaa = 17 common α-amino acids, except Asp, Glu, and Cys; Zaa =19 R1 CH3CO-Gly-Ala, R2 Lys-Phe-Leu-NH2) established criteria for selecting Ser/Thr, Xaa, substitutions optimal specific R1-Ser/Thr peptide bond hydrolysis in the presence Ni(II) ions (Krȩżel, A.; Kopera, E.; Protas, A. M.; Poznański, J.; Wysłouch-Cieszyńska, Bal, W. J. Am. Chem. Soc. 2010, 132, 3355−3366). The screening results were confirmed by...
GPVI (glycoprotein VI) is a key molecular player in collagen-induced platelet signaling and aggregation. Recent evidence indicates that it also plays important role aggregation thrombus growth through interaction with fibrin(ogen). However, there are discrepancies the literature regarding whether monomeric or dimeric form of binds to fibrinogen at high affinity. The mechanisms not clear, including which region responsible for binding. We aimed gain further understanding level identify...
The small leucine-rich proteoglycans (SLRPs) are important regulators of extracellular matrix assembly and cell signalling. We have determined crystal structures at ~2.2Å resolution human fibromodulin chondroadherin, two collagen-binding SLRPs. Their overall fold is similar to that the prototypical SLRP, decorin, but unlike decorin neither nor chondroadherin forms a stable dimer. A previously identified binding site for integrin α2β1 maps an α-helix in C-terminal cap region chondroadherin....
Copper complexes of metal binding domains synthesized amyloid-β peptides – Aβ(1-16) and N-truncated Aβ(4-16) containing a novel N-terminal FRH sequence, as well its shorter mutants were characterized by cyclic voltammetry. The influence the peptide sequence to copper molar ratio on electrochemical properties obtained structures studied discussed. reversibility redox processes in with Aβ(4-x) derivatives was also investigated. results indicate crucial role Tyr10 process complex, including...
Objectives Platelet activation underpins thrombus formation in ischemic stroke. The active, dimeric form of platelet receptor glycoprotein (GP) VI plays key roles by binding ligands collagen and fibrin, leading to activation. We investigated whether patients presenting with stroke expressed more GPVI on their surface had active circulating platelets as measured P-selectin exposure. Methods 129 or hemorrhagic were recruited within 8h symptom onset. Whole blood was analyzed for...
CRNDE is an oncogene expressed as a long non-coding RNA. However, our team previously reported that the gene also encodes micropeptide, CRNDEP. The amino acid sequence of CRNDEP has recently been revealed by other researchers, too. This study aimed to investigate genetic alterations within CRNDEP-coding region gene, methylation profiling this and expression analysis. All investigations were performed on clinical material from patients with ovarian tumors diverse aggressiveness. We found...
Multimerin 1 (human: MMRN1, mouse: Mmrn1) is a homopolymeric, adhesive, platelet and endothelial protein that binds to von Willebrand factor enhances adhesion fibrillar collagen ex vivo.
Silver-based materials are widely used in clinical medicine. Furthermore, the usage of silver containing and devices is recommended clinically approved. The impact on human health increasing use nanoparticles medical remains understudied, even though Ag-containing dressings known to release into bloodstream. In this study, we detected a widespread sometimes significant accumulation both healthy sick liver biopsies, levels being statistically higher patients with various hepatic pathologies....
CRNDE is considered an oncogene expressed as long non-coding RNA. Our previous paper the only one reporting a micropeptide-coding gene. The amino acid sequence of this micropeptide (CRNDEP) has recently been confirmed by other researchers. This study aimed at providing mass spectrometry (MS)-based validation CRNDEP and investigation how differential expression CRNDE(P) influences metabolism chemoresistance ovarian cancer (OvCa) cells. We also assessed cellular localization changes CRNDEP,...
α-Factor-1 (WHWLQLKPGQPMY), a peptidic pheromone of Saccharomyces cerevisiae yeast, contains XHX type copper(II) binding N-terminal site. Using soluble analogue, WHWSKNR-amide, we demonstrated that the W1H2W3 site alone binds with Kd value 0.18 pM at pH 7.4 and also imidazole (Im) in ternary complex (Kd 1 mM 7.4). This interaction boosts ability peptide to sequester depending on Im concentration up subfemtomolar range, not available for any oligopeptidic system studied before. Therefore,...
Ni(ii) ions are able to hydrolyze Naa-(Ser/Thr) peptide bonds in Naa-(Ser/Thr)-Xaa-His-Zaa sequences. We found that various human transcription factors contain such nickel hydrolytic patterns within C2H2 zinc finger (ZF) domains. demonstrated the hydrolysis on two models, 3rd ZF of Sp1 factor and 1st ZNF302 factor. The experimentally studied reaction rates indicate is likely be an element intracellular toxicity.