A5034760490- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Otolaryngology and Infectious Diseases
- Infective Endocarditis Diagnosis and Management
- Diabetic Foot Ulcer Assessment and Management
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
- Cardiovascular and Diving-Related Complications
- Trauma and Emergency Care Studies
- Oral microbiology and periodontitis research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Sepsis Diagnosis and Treatment
- Rabies epidemiology and control
- Inflammatory Bowel Disease
- Diverticular Disease and Complications
- Oral health in cancer treatment
- Glaucoma and retinal disorders
- Inflammasome and immune disorders
- Microscopic Colitis
- Cardiovascular Syncope and Autonomic Disorders
- Urinary Bladder and Prostate Research
- Hypertrophic osteoarthropathy and related conditions
- Intracranial Aneurysms: Treatment and Complications
- Ocular Infections and Treatments
- interferon and immune responses
Region Västra Götaland
2025
Sahlgrenska University Hospital
2015-2025
University of Gothenburg
2013
Abstract Background Necrotizing soft-tissue infections (NSTI) are life-threatening conditions often caused by β-hemolytic streptococci, group A Streptococcus (GAS) in particular. Optimal treatment is contentious. The INFECT cohort includes the largest set of prospectively enrolled streptococcal NSTI cases to date. Methods From 409 adults admitted with 5 clinical centers Scandinavia, patients culture-positive for GAS or dysgalactiae (SD) were selected. Risk factors identified comparison a...
Necrotizing fasciitis caused by group A streptococcus (GAS) is a life-threatening, rapidly progressing infection. At present, biofilm not recognized as potential problem in GAS necrotizing soft tissue infections (NSTI), it typically linked to chronic or associated with foreign devices. Here, we present case of previously healthy male presenting NSTI GAS. The infection persisted over 24 days, and the surgeon documented presence "thick layer biofilm" fascia. Subsequent analysis patient...
Variability in disease severity caused by a microbial pathogen is impacted each infection representing unique combination of host and genomes. Here, we show that the outcome invasive Streptococcus pyogenes regulated an interplay between human STING genotype bacterial NADase activity. S. pyogenes-derived c-di-AMP diffuses via streptolysin O pores into macrophages where it activates ensuing type I IFN response. However, enzymatic activity variants expressed strains suppresses STING-mediated...
Abstract Early assessment of necrotising soft tissue infection (NSTI) is challenging. Analysis inflammatory markers could provide important information about disease severity and guide decision making. For this purpose, we investigated the association between cytokine levels Laboratory Risk Indicator for Necrotising Fasciitis (LRINEC)-score, mortality in NSTI patients. In 159 patients, plasma was analysed IL-1β, IL-6, IL-10 TNF-α upon admission. The assessed by SAPS, SOFA score, septic...
Osteoradionecrosis (ORN) of the mandible is a serious complication head and neck radiotherapy. This study aims to investigate effect hyperbaric oxygen (HBO) treatment on ORN in two randomized, controlled multicentre trials.Patients with indication for surgical were randomised either group 1: removal necrotic mandibular bone supplemented by 30 pre- 10 postoperative HBO exposures at 243 kPa 90 min each, or 2: only. Primary outcome was healing one year after surgery evaluated clinically...
Abstract Background Haemorrhage is a leading cause of morbidity and mortality in trauma, prehospital transfusion blood products often necessary. Whole has been proposed to be the best alternative, but it unclear whether, how, storage transport helicopter affects units. We investigated coagulation capacity platelet function whole at different time points during missions. Methods Twenty units low‐titre group O RhD negative were collected from healthy volunteers analysed before, after...
The M protein is an essential virulence factor of Streptococcus pyogenes , or group A streptococci (GAS), one the most common and dangerous human pathogens. Molecular functional characterization variants their interactions with host components crucial for understanding streptococcal pathogenesis vaccine development. M3 produced by prevalent emm 3 GAS serotype, which frequently associated severe invasive diseases. Here we characterize interaction collagens through detailed structural...
The M protein is an essential virulence factor of Streptococcus pyogenes , or group A streptococci (GAS), one the most common and dangerous human pathogens. Molecular functional characterization variants their interactions with host components crucial for understanding streptococcal pathogenesis vaccine development. M3 produced by prevalent emm 3 GAS serotype, which frequently associated severe invasive diseases. Here we characterize interaction collagens through detailed structural...
Background The INFECT project aims to advance our understanding of the pathophysiological mechanisms in necrotizing soft tissue infections ( NSTI s). observational study is part with aim studying clinical profile patients s and correlating these patient‐important outcomes. With this protocol statistical analysis plan we describe methods used obtain data details planned analyses. Methods a multicentre, prospective cohort study. Patients are enrolled five Scandinavian hospitals, which all...
Abstract Streptococcus dysgalactiae subsp. equisimilis (SDSE) has emerged as an important cause of severe skin and soft tissue infections, but little is known the pathogenic mechanisms underlying pathology. Patient samples a collection invasive non-invasive group G SDSE strains (n = 69) were analyzed with respect to virulence factor expression cytotoxic or inflammatory effects on human cells 3D models. efficiently infected 3D-skin model pathology, responses altered production host structural...
Necrotizing soft tissue infections (NSTIs) are devastating caused by either a single pathogen, predominantly Streptococcus pyogenes, or multiple bacterial species. A better understanding of the pathogenic mechanisms underlying these different NSTI types could facilitate faster diagnostic and more effective therapeutic strategies. Here, we integrate microbial community profiling with host pathogen(s) transcriptional analysis in patient biopsies to dissect pathophysiology streptococcal...
Abstract Analyses of plasma collected pre- and postadministration intravenous immunoglobulin (IVIG) from patients with group A Streptococcus necrotizing soft tissue infections demonstrated a negative correlation between IVIG dose toxin-triggered T-cell proliferation (r = −.67, P < .0001). One 25-g was sufficient to yield plasma-neutralizing activity against streptococcal superantigens. Clinical Trials Registration. NCT 01790698 NCT02111161.
Background and aims. Complementary therapy options are needed in the treatment of active ulcerative colitis (UC). Hyperbaric oxygen (HBOT) has been shown to have positive effects experimental models perianal Crohn's disease. Methods. In present prospective randomized open-label study, HBOT addition conventional medical was compared with alone. The primary objective this study improved clinical outcome evaluated by Mayo score, laboratory tests fecal weight. secondary objectives were...
BACKGROUNDNecrotizing soft-tissue infections (NSTIs) are rapidly progressing frequently complicated by septic shock and associated with high mortality. Early diagnosis is critical for patient outcome, but challenging due to vague initial symptoms. Here, we identified predictive biomarkers NSTI clinical phenotypes outcomes using a prospective multicenter cohort.METHODSLuminex multiplex assays were used assess 36 soluble factors in plasma from patients positive microbiological cultures (n =...
Treatment with hyperbaric oxygen (HBO2) is controversial when treating disorders other than decompression sickness. Still, HBO2 a treatment modality that has gained recognition in certain situations of ischaemia reperfusion. However, not much known about its effect on the endothelial cells. Based earlier studies, hypothesis was stimulates release fibrinolytic factors. The aim study to investigate cultured cells simulated ischaemia-reperfusion model.To mimic clinical situation during...
Abstract Purpose To examine visual acuity, refraction and ocular status before, during after hyperbaric oxygen therapy (HBOT). Methods Twenty‐nine patients underwent 40 standard protocol HBOT treatments. In all subjects, best corrected acuity were measured at baseline ten, 20, 30 treatments, a 12‐week follow‐up. A subgroup ( n = 19) given additional examinations baseline, treatments follow‐up including measuring central corneal thickness (CCT), curvature, anterior chamber depth, lens...
Streptococcus pyogenes (group A streptococci; GAS) is the main causative pathogen of monomicrobial necrotizing soft tissue infections (NSTIs). To resist immuno-clearance, GAS adapt their genetic information and/or phenotype to surrounding environment. Hyper-virulent streptococcal pyrogenic exotoxin B (SpeB) negative variants caused by covRS mutations are enriched during infection. key driving force for this process bacterial Sda1 DNase.Bacterial infiltration, immune cell influx, necrosis and...
Abstract Background Necrotising soft tissue infections (NSTIs) are rapidly progressing bacterial usually caused by either several pathogens in unison (polymicrobial infections) or Streptococcus pyogenes (mono-microbial infection). These rare and associated with high mortality rates. However, the underlying pathogenic mechanisms this heterogeneous group remain elusive. Methods In study, we built interactomes at both population individual levels consisting of host-pathogen interactions...
Necrotizing soft-tissue infections (NSTI) are the most severe form of bacterial-induced tissue pathology. Their unpredictable onset and rapid development into life-threatening conditions considerably complicate patient treatment. Understanding risk factors for NSTI in individual patients is necessary selecting appropriate therapeutic option.We investigated role pathogen-specific antibodies manifestation by performing a comparative serologic approach, using plasma samples bacterial isolates...
Background Necrotising soft‐tissue infections ( NSTIs ) are rare conditions with high morbidity and mortality. Patients often transferred to tertiary hospitals, but the question of whether potential benefits highly specialised care outweigh risks associated inter‐hospital transfers has been raised. Methods Prospective study including all patients treated at intensive unit S ahlgrenska U niversity H ospital/ E ast between J anuary 2008 D ecember 2011. Results Twenty‐nine were identified....