A5102849721- Streptococcal Infections and Treatments
- Immune Response and Inflammation
- Antimicrobial Resistance in Staphylococcus
- Neonatal and Maternal Infections
- Inflammasome and immune disorders
- Bacterial Identification and Susceptibility Testing
- T-cell and B-cell Immunology
- Inflammation biomarkers and pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Sepsis Diagnosis and Treatment
- Antimicrobial Peptides and Activities
- Advanced Glycation End Products research
- Respiratory Support and Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Cell Function and Interaction
- Immune cells in cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Bacterial biofilms and quorum sensing
- Herpesvirus Infections and Treatments
Karolinska Institutet
2016-2023
Karolinska University Hospital
2014-2019
University of Gothenburg
2011
Abstract DNAX accessory molecule-1 (DNAM-1, also known as CD226) is an activating receptor expressed on subsets of natural killer (NK) and T cells, interacts with its ligands CD155 or CD112, has co-varied expression inhibitory receptors. Since receptors control NK-cell activation are necessary for MHC-I-dependent education, we investigated whether DNAM-1 involved in education. Here show correlation between association NKG2A that occurs even MHC class I deficient mice. early during...
Abstract Neutrophils are critical for the control of bacterial infections, but they may also contribute to disease pathology. Here we explore neutrophil responses, in particular release sepsis-associated factors heparin-binding protein (HBP) and resistin relation specific stimuli sepsis varying aetiology. Analyses HBP plasma septic patients revealed elevated levels as compared non-infected critically ill patients. correlated significantly patients, with strongest association seen group A...
Extracellular High Mobility Group Box 1 (HMGB1) has been associated with acute and chronic inflammatory conditions. However, little is known about HMGB1 in necrotizing bacterial infections. We hypothesized that the local response excessive severe soft tissue infections, which are characterized by necrosis hyperinflammation. To explore this, biopsies were collected from patients varying severity of Streptococcus pyogenes skin including erysipelas, cellulitis, fasciitis. Tissue sections...
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of skin and soft tissue infections. One the highly successful rapidly disseminating clones MRSA ST22 commonly associated with tropism. Here we show that naturally occurring single amino acid substitution (tyrosine to cysteine) at position 223 AgrC determines starkly different S. virulence phenotypes, e.g. cytotoxic or colonizing, as evident in both vitro vivo Y223C destabilizes AgrC-AgrA interaction leading colonizing...
Necrotizing soft tissue infections (NSTIs) are devastating caused by either a single pathogen, predominantly Streptococcus pyogenes, or multiple bacterial species. A better understanding of the pathogenic mechanisms underlying these different NSTI types could facilitate faster diagnostic and more effective therapeutic strategies. Here, we integrate microbial community profiling with host pathogen(s) transcriptional analysis in patient biopsies to dissect pathophysiology streptococcal...
Tuberculosis (TB) and HIV co-infection claims many lives every year. This study assessed immune responses in Mycobacterium tuberculosis-infected lymph node tissues from HIV-negative HIV-positive patients compared with the peripheral circulation a focus on myeloid cells cell-signaling enzymes, inducible nitric oxide synthase, arginase (Arg)-1. Methods included immunohistochemistry or confocal microscopy computerized image analyses, quantitative real-time PCR, multiplex Luminex, flow...
Innate immunity relies on an effective recognition of the pathogenic microorganism as well endogenous danger signals. While bacteria in concert with their secreted virulence factors can cause a number inflammatory reactions, signals released at site infection may addition determine amplitude such responses, and influence outcome disease. Here we report that protein SIC, Streptococcal Inhibitor Complement, abundant from Streptococcus pyogenes, binds to extracellular histones, group during...
Abstract Background Necrotising soft tissue infections (NSTIs) are rapidly progressing bacterial usually caused by either several pathogens in unison (polymicrobial infections) or Streptococcus pyogenes (mono-microbial infection). These rare and associated with high mortality rates. However, the underlying pathogenic mechanisms this heterogeneous group remain elusive. Methods In study, we built interactomes at both population individual levels consisting of host-pathogen interactions...
The formation of neutrophil extracellular traps (NETs) is a host defence mechanism, known to facilitate the entrapment and growth inhibition many bacterial pathogens. It has been implicated that translocation myeloperoxidase (MPO) from neutrophilic granules nucleus crucial this process. Under disease conditions, however, excessive NET can trigger self-destructive complications by releasing pathologic levels danger-associated molecular pattern molecules (DAMPs). To counteract such devastating...
ABSTRACT Staphylococcus aureus is an important human pathogen causing severe invasive infections. Pathogenesis attributed to a wide array of virulence factors, including several potent exotoxins such as the pore-forming alpha-toxin. In this study, we found that patients with S. respiratory tract infections had elevated CX 3 CL1 levels in airway fluid and plasma. Using humanized organotypic lung models, observed stimulation epithelium alpha-toxin induce intensified expression apically well...