A5048548750- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Mesenchymal stem cell research
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Diabetes and associated disorders
- FOXO transcription factor regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic function and diabetes
- Malaria Research and Control
- Complement system in diseases
- Diabetes Management and Research
- Monoclonal and Polyclonal Antibodies Research
- Phagocytosis and Immune Regulation
- Tissue Engineering and Regenerative Medicine
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Microfluidic and Bio-sensing Technologies
- vaccines and immunoinformatics approaches
- Cancer-related cognitive impairment studies
- Reproductive System and Pregnancy
- Hematopoietic Stem Cell Transplantation
- Phytoestrogen effects and research
- Protein Degradation and Inhibitors
Karolinska Institutet
2016-2025
Karolinska University Hospital
2016-2022
Science for Life Laboratory
2019-2022
University of Gothenburg
2008-2012
Oniris
2006
Nantes Université
2006
Abstract Mesenchymal stromal cells (MSCs) exert broad immunosuppressive potential, modulating the activity of innate and adaptive immune systems. As MSCs become accepted as a therapeutic option for treatment immunological disorders such Graft versus Host Disease, our need to understand intricate details by which they their effects is crucial. Programmed death-1 (PD-1) an important regulator in T cell activation homeostatic control. It has been reported that this pathway may be...
We provide the first comprehensive analysis of extracellular matrix (ECM) composition peri-islet capsules, composed basement membrane (BM) and subjacent interstitial (IM), in development type 1 diabetes NOD mice human diabetes. Our data demonstrate global loss BM IM components only at sites leukocyte infiltration into islet. Stereological analyses reveal a correlation between incidence insulitis number islets showing versus with intact BMs, suggesting that penetration is critical step....
Journal Article Live cell imaging in a micro-array of acoustic traps facilitates quantification natural killer heterogeneity Get access Athanasia E. Christakou, Christakou Department Applied Physics, Albanova University Center, KTH - Royal Institute Technology, SE-10691, Stockholm, Sweden. Tel: +46 8 55378793, 730 766146 Search for other works by this author on: Oxford Academic Google Scholar Mathias Ohlin, Ohlin Bruno Vanherberghen, Vanherberghen Mohammad Ali Khorshidi, Khorshidi Nadir...
Abstract DNAX accessory molecule-1 (DNAM-1, also known as CD226) is an activating receptor expressed on subsets of natural killer (NK) and T cells, interacts with its ligands CD155 or CD112, has co-varied expression inhibitory receptors. Since receptors control NK-cell activation are necessary for MHC-I-dependent education, we investigated whether DNAM-1 involved in education. Here show correlation between association NKG2A that occurs even MHC class I deficient mice. early during...
Steroid-refractory chronic graft-vs-host disease (cGvHD) contributes to morbidity after allogeneic hematopoietic stem cell transplantation. Here, we report on 11 patients with severe, refractory cGvHD treated repeated infusions of bone marrow-derived mesenchymal stromal cells (MSC) over a 6- 12-month period. Six responded MSC treatment following National Institutes Health response criteria, accompanied by improvement in GvHD-related symptoms and quality life. This was durable, systemic...
Abstract Aims/hypothesis We and others previously reported the presence of tertiary lymphoid organs (TLOs) in pancreas NOD mice, where they play a role development type 1 diabetes. Our aims here are to investigate whether TLOs present individuals with diabetes characterise their distinctive features, comparison mouse pancreases, order interpret functional significance. Methods Using immunofluorescence confocal microscopy, we examined extracellular matrix (ECM) cellular constituents...
Natural killer (NK) cells recognize virally infected and tumors. NK cell function depends on balanced signaling from activating receptors, recognizing products tumors or viruses, inhibitory receptors (such as KIR/Ly49), which major histocompatibility complex class I (MHC-I) molecules. KIR/Ly49 preserves tolerance to self but also conveys reactivity toward MHC-I–low target in a process known education. Here, we found that education were determined by the subcellular localization of tyrosine...
Type 1 diabetes (T1D) is a chronic autoimmune disease that results from T cell-mediated destruction of pancreatic β cells. CD1d-restricted NKT lymphocytes have the ability to regulate immunity, including autoimmunity. We previously demonstrated type II cells, which carry diverse TCRs, prevented T1D in NOD mouse model for human disease. In this study, we show CD4(+) 24αβ but not CD4/CD8 double-negative were sufficient downregulate diabetogenic BDC2.5 cells adoptive transfer experiments....
Abstract Multiple sclerosis ( MS ) is a chronic autoimmune disease of the central nervous system involving dysregulated encephalitogenic T cells. Myeloid‐derived suppressor cells MDSC s) have been recognized for their important function in regulating T‐cell responses. Recent studies indicated role s diseases, but significance not clear. Here, we assessed frequencies CD 14 + HLA ‐ DR low monocytic (Mo‐ and 33 15 11b granulocytic (Gr‐ investigated phenotypic functional differences Mo‐ at...
Mesenchymal stromal cell (MSC) therapy is a promising tool in the treatment of chronic inflammatory diseases. This has been ascribed to capacity MSC release large variety immune-modulatory factors. However, all aspects mode therapeutic action different diseases remain unresolved, mainly because most infused are undetectable circulation within hours after infusion. The aim this study was elucidate fate contact with plasma. We found that upon blood, complement proteins including C3b/iC3b...
The cochlea possesses a robust circadian clock machinery that regulates auditory function. How the cochlear is influenced by system remains unknown. Here, we show rhythms are driven and require local Bmal1 as well central input from suprachiasmatic nuclei (SCN). SCN ablations disrupted expression of core genes in cochlea. Because secretion glucocorticoids (GCs) controlled GCs known to modulate function, assessed their influence on gene expression. Removal circulating adrenalectomy (ADX) did...
BACKGROUND The presence of antibodies against HLA Class I can lead to platelet (PLT) transfusion refractoriness, that is, the repeated failure achieve adequate posttransfusion PLT count increments. refractoriness be overcome by HLA‐matched donor PLTs. A different approach is remove from surface using low pH. Previous case studies HLA‐stripped PLTs showed encouraging but inconsistent results and lacked information on biologic effects acid treatment function as well sensitivity destruction in...
Bone marrow derived mesenchymal stromal cells (BM-MSCs) have emerged as a possible new therapy for Multiple Sclerosis (MS), however studies regarding efficacy and in vivo immune response been limited inconclusive. We conducted phase I clinical study assessing safety peripheral responses after MSC MS. Seven patients with progressive MS were intravenously infused single dose of autologous (1–2 × 106 MSCs/kg body weight). The infusions safe well tolerated when given during remission. Five out...
Over the last two decades, use of adult stem cells in therapy has gained significant momentum. However, are usually associated with high extraction, expansion and storage costs. This is delaying their approval into clinical practice. By repurposing medical waste tissues for cell harvesting, there an opportunity to extract valuable therapeutic material without incurring additional costs procuring raw materials or handling disposal. Harvesting from discarded a non-invasive, safe procedure that...
The compromise between affinity and specificity in TCR-dependent targeting of HLA-restricted tumor-associated antigens presents a significant challenge developing efficacious immunotherapies. As such, T cell engagers which circumvent these limitations are particular interest. We have established process to generate bispecific Designed Ankyrin Repeat Proteins (DARPins) that simultaneously target HLA-I molecules complex with peptides CD3ϵ. High-affinity HLA-A*0201/NY-ESO1 157-165 -specific...
Natural immunity to malaria develops over time with repeated episodes, but protection against severe and immune regulation limiting immunopathology, called tolerance, more rapidly. Here, we comprehensively profile the blood system in patients, or without prior exposure, 1 year after acute symptomatic Plasmodium falciparum malaria. Using a data-driven analysis approach describe landscape time, show that dampened inflammatory response is associated reduced γδ T cell expansion, early expansion...