A5060909523- Malaria Research and Control
- Immunotoxicology and immune responses
- Complement system in diseases
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Animal testing and alternatives
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Carcinogens and Genotoxicity Assessment
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Infectious Diseases and Mycology
- Transgenic Plants and Applications
- T-cell and B-cell Immunology
- Entomopathogenic Microorganisms in Pest Control
- Research on Leishmaniasis Studies
- Invertebrate Immune Response Mechanisms
- Pesticide Exposure and Toxicity
- Insect and Arachnid Ecology and Behavior
- Actinomycetales infections and treatment
- Plant and animal studies
- Glycosylation and Glycoproteins Research
- Chemokine receptors and signaling
- Phytochemical compounds biological activities
Karolinska Institutet
2021-2024
Karolinska University Hospital
2021-2024
ETH Zurich
2012-2022
Board of the Swiss Federal Institutes of Technology
2022
Fundación Instituto de Inmunología de Colombia
2007-2020
Universidad del Rosario
2019
Universidad Nacional de Colombia
2007-2011
Heidelberg University
2005
Institut für Medizinische Biometrie, Informatik und Epidemiologie
2005
It is well known that mushrooms produce defense proteins and secondary metabolites against predators competitors; however, less about the correlation between tissue-specific expression target organism (antagonist) specificity of these molecules. In addition, conserved transcriptional circuitries involved in developing sexual organs fungi are not characterized, despite growing number gene datasets available from reproductive vegetative tissue. The aims this study were: first, to evaluate...
The modification of α1,6-linked fucose residues attached to the proximal (reducing-terminal) core N-acetylglucosamine residue N-glycans by β1,4-linked galactose ("GalFuc" epitope) is a feature number invertebrate species including model nematode Caenorhabditis elegans. A pre-requisite for both α1,6-fucosylation and β1,4-galactosylation presence nonreducing terminal N-acetylglucosamine; however, this normally absent from final glycan structure in invertebrates due action specific...
Abstract The dung of herbivores, the natural habitat model mushroom Coprinopsis cinerea, is a nutrient-rich but also very competitive environment for saprophytic fungus. We showed previously that C. cinerea expresses constitutive, tissue-specific armories against antagonists such as animal predators and bacterial competitors. In order to dissect inducible antagonists, we sequenced poly(A)-positive transcriptome vegetative mycelium upon challenge with fungivorous bacterivorous nematodes,...
Our results support the hypothesis that cytoplasmic proteins abundant in fungal fruiting bodies are involved resistance against predation. The toxicity of these toward stylet-feeding nematodes, which also capable feeding on plants, and abundance edible mushrooms, may open possible avenues for biological crop protection parasitic e.g., by expression crops.
Natural immunity to malaria develops over time with repeated episodes, but protection against severe and immune regulation limiting immunopathology, called tolerance, more rapidly. Here, we comprehensively profile the blood system in patients, or without prior exposure, 1 year after acute symptomatic Plasmodium falciparum malaria. Using a data-driven analysis approach describe landscape time, show that dampened inflammatory response is associated reduced γδ T cell expansion, early expansion...
Abstract Rationale: Nocardia species are not commonly referred as primary infectious entities but rather opportunistic pathogens. Infectious cases of spp. in immunocompetent individuals rare. Patient concerns: An 58-year-old patient presented with recurrent headaches. Diagnosis: A brain abscess was found and surgically drained. Matrix-assisted laser desorption ionization–time-of-flight mass spectrometry heat shock protein 65/16S-23S rRNA gene intergenic spacer genotyping from the sample...
Lectins are non-immunoglobulin-type proteins that bind to specific carbohydrate epitopes and play important roles in intra- inter-organismic interactions. Here, we describe a novel fucose-specific lectin, termed CML1, which identified from fruiting body extracts of Coprinopsis cinerea. For further characterization, the coding sequence for CML1 was cloned heterologously expressed Escherichia coli. Feeding CML1-producing bacteria inhibited larval development bacterivorous nematode...
The characterization of membrane proteins having no identified function in Mycobacterium tuberculosis is important for a better understanding the biology this pathogen. In work, biological activity Rv2560 protein was characterized and evaluated. Primers used PCR RT‐PCR assays revealed that gene encoding present M. complex strains, but transcribed only some them. Sera obtained from rabbits inoculated with polymer peptides recognized 33 kDa band lysate fraction corresponding to predicted...
The Rv2707 gene encoding a putative alanine- and leucine-rich protein was found to be present in all Mycobacterium tuberculosis complex strains (by PCR) its transcription shown by RT-PCR but M. bovis microti. Antibodies raised against peptides specifically recognized the native Western blot were able locate this on membrane immunoelectron microscopy. A549 U937 cells lines used binding assays involving synthetic covering whole protein. High cell-binding peptide 16083 (281...
Malaria remains a significant burden, and fully protective vaccine against
We introduce a new family of fungal protease inhibitors with β-trefoil fold from the mushroom Coprinopsis cinerea, named cocaprins, which inhibit both cysteine and aspartic proteases. Two cocaprin-encoding genes are differentially expressed in tissues. One is highly transcribed vegetative mycelium other stipes mature fruiting bodies. Cocaprins small proteins (15 kDa) acidic isoelectric points that form dimers. The three-dimensional structure cocaprin 1 showed similarity to lectins. plant C1...
Many vaccine candidate proteins in the malaria parasite Plasmodium falciparum are under strong immunological pressure and confer antigenic diversity. We present a sequencing data analysis platform for genomic surveillance of insertion or deletion (indel)-rich antigens merozoite surface protein 1 (MSP1), MSP2, glutamate-rich (GLURP), CSP from P. using long-read circular consensus (CCS) multiclonal isolates. Our uses 40 PCR primers per gene to asymmetrically barcode identify infections pools...
Evaluating immunomodulating effects is currently a fundamental parameter when designing pharmaceutical products. Rather than wait for the appearance of immunotoxic in patients, pre-clinical assays that characterize these potential events experimental models not only can facilitate understanding how phenomena arise, but sometimes reveal findings lead to modifications currently-accepted methodologies used estimating assay predictive risk values. Pentamidine isethionate, drug as second option...
Malaria remains a significant burden, and fully protective vaccine against Plasmodium falciparum is critical for reducing morbidity mortality. Antibody responses the blood-stage antigen merozoite surface protein 2 (MSP2) are associated with protection from P. malaria, but its extensive polymorphism barrier to development as candidate. New tools, such long-read sequencing accurate structure modelling allow us more easily study genetic diversity immune towards antigens clinical isolates. This...