A5047529642- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Long-Term Effects of COVID-19
- Biosensors and Analytical Detection
- T-cell and B-cell Immunology
- Systemic Lupus Erythematosus Research
- SARS-CoV-2 and COVID-19 Research
- Glycosylation and Glycoproteins Research
- GDF15 and Related Biomarkers
- Genetics, Aging, and Longevity in Model Organisms
- Bioinformatics and Genomic Networks
- Immunodeficiency and Autoimmune Disorders
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- Rheumatoid Arthritis Research and Therapies
- Ion channel regulation and function
- Gene expression and cancer classification
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Metabolomics and Mass Spectrometry Studies
- Asthma and respiratory diseases
- HIV Research and Treatment
- Malaria Research and Control
KTH Royal Institute of Technology
2015-2024
Science for Life Laboratory
2015-2024
AlbaNova
2009
Abstract SARS-CoV-2 may pose an occupational health risk to healthcare workers. Here, we report the seroprevalence of antibodies, self-reported symptoms and exposure among workers at a large acute care hospital in Sweden. The IgG antibodies against was 19.1% 2149 recruited between April 14th May 8th 2020, which higher than reported regional during same time period. Symptoms associated with were anosmia (odds ratio (OR) 28.4, 95% CI 20.6–39.5) ageusia (OR 19.2, 14.3–26.1). Seroprevalence also...
Autoimmune diseases disproportionately affect females more than males. The XX sex chromosome complement is strongly associated with susceptibility to autoimmunity. Xist long non-coding RNA (lncRNA) expressed only in randomly inactivate one of the two X chromosomes achieve gene dosage compensation. Here, we show that ribonucleoprotein (RNP) complex comprising numerous autoantigenic components an important driver sex-biased Inducible transgenic expression a non-silencing form male mice...
Significance Despite the growing evidence that autoantibodies are team players in pathogenesis of multiple sclerosis (MS), target autoantigens yet to be identified. In this work, we mined autoantibody repertoire within MS by screening more than 2,000 plasma samples from patients with and controls identified increased reactivity against an ion-channel protein called “anoctamin 2” (ANO2). This finding points toward ANO2 autoimmune sub-phenotype might contribute development clinical algorithms...
Identification of target antigens PLA2R, THSD7A, NELL1, or Semaphorin-3B can explain the majority cases primary membranous nephropathy (MN). However, remain unidentified in 15%-20% patients.A multipronged approach, using traditional and modern technologies, converged on a novel antigen, capitalized temporal variation autoantibody titer for biomarker discovery. Immunoblotting human glomerular proteins followed by differential immunoprecipitation mass spectrometric analysis was complemented...
Autoantibodies have been shown to be implied in COVID-19 but the emerging autoantibody repertoire remains largely unexplored. We investigated new-onset 525 healthcare workers and hospitalized patients at five time points over a 16-month period 2020 2021 using proteome-wide targeted protein peptide arrays. Our results show that prevalent autoantibodies against wide range of antigens emerged following SARS-CoV-2 infection relation pre-infectious baseline samples remained elevated for least 12...
The COVID-19 pandemic poses an immense need for accurate, sensitive and high-throughput clinical tests, serological assays are needed both overarching epidemiological studies evaluating vaccines. Here, we present the development validation of a multiplex bead-based assay.More than 100 representations SARS-CoV-2 proteins were included initial evaluation, including antigens produced in bacterial mammalian hosts as well synthetic peptides. five best-performing antigens, three representing spike...
Protein microarrays are useful tools for highly multiplexed determination of presence or levels clinically relevant biomarkers in human tissues and biofluids. However, such have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy use lateral flow protein microarray device capable sensitive (< 30 ng/mL) antigen-specific antibodies ten minutes total assay time. Results were developed with gold nanobeads could be recorded by cell-phone camera table...
Abstract Background SARS-CoV-2 may pose an occupational health risk to care workers, but the prevalence of infections in this population is unknown. We examined seroprevalence antibodies among workers at a large acute hospital Stockholm, Sweden. determined correlations between seroprevalence, self-reported symptoms and exposure SARS-CoV-2. Methods findings All employees Danderyd Hospital (n=4375) were invited participate cross-sectional study. 2149 from all departments enrolled study April...
We demonstrate a novel dual-cure polymer featuring long shelf life with direct room-temperature bonding, and its application in protein microarrays.
Abstract Autoantibodies have been shown to be implied in COVID-19 but the emerging autoantibody repertoire remains largely unexplored. We investigated new-onset 525 healthcare workers and hospitalized patients five time points over 16 months using proteome-wide targeted protein peptide arrays. Our results show that prevalent autoantibodies against a wide range of antigens emerged following SARS-CoV-2 infection relation pre-infectious baseline samples remained elevated for at least 12 months....
Background Dried blood spot samples (DBSS) from newborns are widely used in neonatal screening for selected metabolic diseases and diagnostic possibilities additional disorders continuously being evaluated. Primary immunodeficiency comprise a group of more than one hundred diseases, several which fatal early life. Yet, majority the patients not diagnosed due to lack high-throughput methods. Methodology/Principal Findings We have previously developed system using reverse phase protein...
Abstract Dogs can spontaneously develop complex systemic autoimmune disorders, with similarities to human disease. Autoantibodies directed at self-antigens are a key feature of these diseases. Here we report the identification interleukin enhancer-binding factors 2 and 3 (ILF2 ILF3) as autoantigens in canine immune-mediated rheumatic The ILF2 autoantibodies were discovered small, selected cohort through use protein arrays; method not previously described dogs. Subsequently, ILF3 also...
Affinity proteomic approaches by antibody bead arrays enable multiplexed analysis of proteins in body fluids. In the presented study, we investigated blood plasma within osteoporosis to discovery differential protein profiles and propose novel biomarkers candidates for subsequent studies.Starting with 4608 antibodies samples from 22 women an untargeted screening, a set 72 were suggested further analysis. Complementing these targets literature other studies, targeted array 180 was built...
The interaction of allergens and allergen-specific IgE initiates the allergic cascade after crosslinking receptors on effector cells. Antibodies other isotypes may modulate such a reaction. Receptor requires binding antibodies to multiple epitopes allergen. Limited information is available complexity epitope structure most allergens.We sought allow description IgE, IgG4, IgG recognition at global, allergome-wide level during immunotherapy (AIT).We generated an microarray comprising 731 in...
Despite recognizing aging as a common risk factor of many human diseases, little is known about its molecular traits. To identify age-associated proteins circulating in blood, we screened 156 individuals aged 50–92 using exploratory and multiplexed affinity proteomics assays. Profiling eight additional study sets (N = 3,987), performing antibody validation, conducting meta-analysis revealed consistent age association ( P 6.61 × 10 −6 ) for histidine-rich glycoprotein (HRG). Sequence variants...
Malaria remains a significant burden, and fully protective vaccine against
Allergic diseases affect many individuals world-wide and are dependent on the interaction between allergens antibodies of IgE isotype. Allergen-specific immunotherapy (AIT) can alter development disease, e.g., through induction allergen-specific IgG that block allergen-IgE interactions. The knowledge epitopes recognized by allergy-causing protective limited. Therefore, we developed an allergome-wide peptide microarray, aiming to track linear epitope binding patterns in allergic during AIT....
Rheumatic diseases are high prevalence pathologies with different etiology and evolution low sensitivity in clinical diagnosis. Therefore, it is necessary to develop an early diagnosis method which allows personalized treatment, depending on the specific pathology. The biology/disease initiative, at Human Proteome Project, integrative approach identify relevant proteins human proteome associated pathologies. A previously reported literature data mining analysis, identified related...
The reverse phase serum microarray format enables multi-parallel and simultaneous analysis of literally thousands samples, a feature which is uttermost importance for protein profiling clinical samples. We have here screened 2400 samples their potential IgA deficiency by using fluorescence based platform biosensor label-free verification also compared our microarray-results to routine ELISA. been able identify possible IgA-deficiencies show the suitability microarray-platforms large-scale...