A5024022376- Alzheimer's disease research and treatments
- Amyotrophic Lateral Sclerosis Research
- SARS-CoV-2 and COVID-19 Research
- Prion Diseases and Protein Misfolding
- Neuroinflammation and Neurodegeneration Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Cerebrospinal fluid and hydrocephalus
- Dementia and Cognitive Impairment Research
- Bioinformatics and Genomic Networks
- Neurogenetic and Muscular Disorders Research
- Advanced Proteomics Techniques and Applications
- SARS-CoV-2 detection and testing
- Spinal Dysraphism and Malformations
- Tryptophan and brain disorders
- Monoclonal and Polyclonal Antibodies Research
- Immunodeficiency and Autoimmune Disorders
- Functional Brain Connectivity Studies
- COVID-19 and healthcare impacts
- Ion Transport and Channel Regulation
- Chemotherapy-induced cardiotoxicity and mitigation
- Chronic Lymphocytic Leukemia Research
- COVID-19 Clinical Research Studies
- Advanced Neuroimaging Techniques and Applications
- Neonatal and fetal brain pathology
- Biosensors and Analytical Detection
KTH Royal Institute of Technology
2021-2025
Science for Life Laboratory
2021-2025
Abstract Background Amyloid and tau aggregates are considered to cause neurodegeneration consequently cognitive decline in individuals with Alzheimer’s disease (AD). Here, we explore the potential of cerebrospinal fluid (CSF) proteins reflect AD pathology decline, aiming identify biomarkers for monitoring outcomes disease-modifying therapies targeting these aggregates. Method We used a multiplex antibody-based suspension bead array measure levels 49 CSF from Swedish GEDOC memory clinic...
Immunocompromised individuals are highly susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Whether vaccine-induced immunity in these involves oral cavity, a primary site of infection, is presently unknown.Immunocompromised patients (n = 404) and healthy controls 82) participated prospective clinical trial (NCT04780659) encompassing two doses the mRNA BNT162b2 vaccine. Primary immunodeficiency (PID), secondary immunodeficiencies caused by human virus (HIV)...
Neurodegenerative dementias are progressive diseases that cause neuronal network breakdown in different brain regions often because of accumulation misfolded proteins the extracellular matrix, such as amyloids or inside neurons other cell types brain. Several diagnostic protein biomarkers body fluids being used and implemented, for Alzheimer's disease. However, there is still a lack co-pathologies causes dementia. Such biofluid-based enable precision medicine approaches diagnosis treatment,...
Abstract Accurate diagnosis and monitoring of neurodegenerative diseases require reliable biomarkers. Cerebrospinal fluid (CSF) proteins are promising candidates for reflecting brain pathology; however, their diagnostic utility may be compromised by natural variability between individuals, weakening association with disease. Here, we measured the levels 69 pre-selected in cerebrospinal using antibody-based suspension bead array technology a multi-disease cohort 499 individuals disorders...
We studied clinical and immunological outcome of Covid-19 in consecutive CLL patients from a well-defined area during month 1-13 the pandemic. Sixty (median age 71 y, range 43-97) were identified. Median CIRS was eight (4-20). Patients had indolent (n = 38), completed 12) or ongoing therapy 10). Forty-six (77%) hospitalized due to severe 11 admitted ICU. Severe equally distributed across subgroups irrespective age, gender, BMI, status except (p < 0.05). Fourteen (23%) died; ≥75 y only...
The COVID-19 pandemic poses an immense need for accurate, sensitive and high-throughput clinical tests, serological assays are needed both overarching epidemiological studies evaluating vaccines. Here, we present the development validation of a multiplex bead-based assay.More than 100 representations SARS-CoV-2 proteins were included initial evaluation, including antigens produced in bacterial mammalian hosts as well synthetic peptides. five best-performing antigens, three representing spike...
Alzheimer's disease and related dementias have a multifactorial aetiology heterogeneous biology. The current study aims to identify different biological signatures in deeply phenotyped memory clinic patient population. In this cross-sectional study, we analysed 49 pre-specified proteins using multiplex antibody-based suspension bead array 278 CSF samples from the real-world research database biobank at Karolinska University Hospital Memory Clinic, Solna, Sweden. Patients with clinical...
Proteomics studies have shown differential expression of numerous proteins in dementias but rarely led to novel biomarker tests for clinical use. The Marie Curie MIRIADE project is designed experimentally evaluate development strategies accelerate the validation and ultimate implementation biomarkers practice, using proteomics-based main as experimental case studies. We address several knowledge gaps that been identified field. First, there technology-translation gap different technologies...
To evaluate the prognostic potential of neurofilament medium chain (NfM) in CSF from patients with ALS and explore its relationship extensively studied light (NfL) phosphorylated heavy (pNfH).
Highly accurate serological tests are key to assessing the prevalence of SARS-CoV-2 antibodies and level immunity in population. This is important predict current future status pandemic. With recent emergence new more infectious variants, assays allowing for high throughput analysis able neutralize become even important. Here, we report development validation a robust, method, which enables assessment inhibiting binding between spike protein angiotensin converting enzyme 2 (ACE2). The assay...
Abstract Background Accurate diagnosis and monitoring of neurodegenerative diseases requires reliable biomarkers. Cerebrospinal fluid (CSF) proteins hold promise for reflecting brain pathology, yet their utility may be compromised by natural variability between individuals, weakening association with disease impacting diagnostic accuracy. Method We measured the levels 69 in CSF from 499 individuals using an antibody-based suspension bead array technology. In this multi-disease cohort...
ABSTRACT Importance Aquaporin-4 (AQP4) plays a critical role in the glymphatic system, responsible for clearing brain solutes like Aβ peptides. Exploring AQP4 as an Alzheimer’s disease (AD) biomarker might aid understanding of AD neuropathology and monitor effects novel drug candidates on system. Objective To determine potential CSF early stage using newly established immunoassay. Design A discovery cohort (n = 157) (2010-2022), composed by patients, other neurodegenerative conditions...
Abstract Objective To evaluate the prognostic potential of neurofilament medium chain (NfM) in CSF from patients with ALS and explore its relationship extensively studied light (NfL) phosphorylated heavy (pNfH). Method levels NfL, NfM, pNfH were analysed 235 samples ALS, mimics, healthy controls a well-characterized cohort Karolinska Clinical Research Centre Stockholm, Sweden. NfM using an antibody-based suspension bead-array NfL measured standard ELISA. data, including Revised Functional...
Abstract Background The effect of varying brain ventricular volume on the cerebrospinal fluid (CSF) proteome has been discussed as possible confounding factors in comparative protein level analyses. However, relationship between CSF and levels remains largely unexplored. Moreover, few existing studies provide conflicting findings, indicating need for further research. Methods Here, we explored association 88 pre-selected proteins derived from magnetic resonance imaging (MRI) measurements 157...
Abstract Background The heterogeneity of a multifactorial disease like Alzheimer’s (AD) cannot be captured solely with ATN biomarkers. Previous research has identified mixed neuropathologies as well subtypes discovered by brain scans and cerebrospinal fluid (CSF) proteomics in AD cohorts. Still, we need better understanding how clinical characteristics distribute between subgroups AD. Additionally, various approaches determining to evaluated. AD, which falls under the umbrella term dementia,...