A5029512802- Malaria Research and Control
- HIV Research and Treatment
- Mosquito-borne diseases and control
- vaccines and immunoinformatics approaches
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Complement system in diseases
Karolinska University Hospital
2022-2024
Karolinska Institutet
2022-2024
Malaria remains a significant burden, and fully protective vaccine against
Many vaccine candidate proteins in the malaria parasite Plasmodium falciparum are under strong immunological pressure and confer antigenic diversity. We present a sequencing data analysis platform for genomic surveillance of insertion or deletion (indel)-rich antigens merozoite surface protein 1 (MSP1), MSP2, glutamate-rich (GLURP), CSP from P. using long-read circular consensus (CCS) multiclonal isolates. Our uses 40 PCR primers per gene to asymmetrically barcode identify infections pools...
Malaria remains a significant burden, and fully protective vaccine against Plasmodium falciparum is critical for reducing morbidity mortality. Antibody responses the blood-stage antigen merozoite surface protein 2 (MSP2) are associated with protection from P. malaria, but its extensive polymorphism barrier to development as candidate. New tools, such long-read sequencing accurate structure modelling allow us more easily study genetic diversity immune towards antigens clinical isolates. This...
Abstract Many vaccine candidate proteins are under strong selective pressure to diversify in terms of antigenicity. We present a sequencing and data analysis platform for epidemiological surveillance discovery indel-rich antigens by long-read circular consensus (CCS) multiclonal pathogen isolates. Our uses 40 PCR primers asymmetrically barcode identify infections pools up 384 samples. validated the method using 235 mock combining 10 synthetic variants gene merozoite surface protein 2...