A5034041240- Platelet Disorders and Treatments
- Proteins in Food Systems
- Blood properties and coagulation
- Meat and Animal Product Quality
- Microbial Inactivation Methods
- Food composition and properties
- Antiplatelet Therapy and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Protein Hydrolysis and Bioactive Peptides
- Biofuel production and bioconversion
- Blood groups and transfusion
- Listeria monocytogenes in Food Safety
- Blood Coagulation and Thrombosis Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Bee Products Chemical Analysis
- Phytase and its Applications
- Food Chemistry and Fat Analysis
- Protease and Inhibitor Mechanisms
- Microencapsulation and Drying Processes
- Inflammatory Biomarkers in Disease Prognosis
- Biodiesel Production and Applications
- Insect Utilization and Effects
- Enzyme Catalysis and Immobilization
- Hormonal and reproductive studies
Princeton University
2024
California Polytechnic State University
2015-2023
University of Cambridge
2012-2023
Iowa State University
2006-2018
Seoul National University Hospital
2015
Kurume University
2000-2010
Konyang University
2010
Kaketsuken (Japan)
2005
Scripps Research Institute
2005
Deutsche Forschungsgemeinschaft
2004
Molecular level studies on platelets deficient in collagen-induced aggregation provide evidence for identifying possible platelet collagen receptors. We investigated from a patient with mild bleeding time prolongation, but otherwise normal coagulation data. Her lacked and adhesion, retained release by other agonists. Labeling her 125I or 3H analysis SDS-PAGE/autoradiography showed levels of glycoproteins Ia, Ib, IIa, IIb, IIIa, IV. However, there were significantly decreased incorporations...
Abstract The Kjeldahl and Dumas methods for quantifying nitrogen content were compared using nine soybean products having protein contents ranging from 0.5 to 90%. In addition comparing day‐to‐day variability of the method, differences between variabilities two systems operators also evaluated. method gave slightly, but significantly, lower values than did method. Both had equivalent (same SD about means). ratios means (K/D) ranged 0.66 1.03. conversion equation y =−0.00536+0.97188 x ( R 2...
ABSTRACT: Endo-protease treatments achieving low degrees of hydrolysis (DH 2% and 4%) were used to improve functional properties hexane-extracted soy flour (HESF), extruded-expelled partially defatted (EESF), ethanol-washed protein concentrate (SPC), isolate (SPI). These substrates had dispersibility indices ranging from 11% 89%. Functional properties, including solubility profile (pH 3 7), emul-sification capacity stability, foaming apparent viscosity determined related surface...
Abstract Aqueous extraction processing technologies, having advanced in recent years, may be a viable alternative to hexane separate oil and protein from soybeans. Different strategies incorporating various modes of comminution, buffers, enzymes allow production range products, but also create different challenges. Processes capable achieving high free yields often result soluble fraction difficult isolate dilute emulsions break. Other processes can achieve purities native soy protein, with...
Abstract The effects of two commercial endoproteases (Protex 6L and Protex 7L, Genencor Division Danisco, Rochester, NY, USA) on the oil protein extraction yields from extruded soybean flakes during enzyme‐assisted aqueous processing (EAEP) were evaluated. Oil distributed in three fractions generated by EAEP: cream + free oil, skim insolubles. was more effective for extracting total solids than 7L. 96 85%, respectively, obtained using 0.5% 6L. Enzymatic pH treatments evaluated to de‐emulsify...
Essentials Dimeric high-affinity collagen receptor glycoprotein VI (GPVI) is present on resting platelets. Spatio-temporal organization of platelet GPVI-dimers was evaluated using advanced microscopy. Upon adhesion to collagenous substrates, coalesce form clusters. Clustering may increase avidity and facilitate activation SUMMARY: Background Platelet binding subendothelial exposed upon blood vessel injury initiates thrombus formation. GPVI has high affinity for collagen, occurs...
Nucleotide-based drug candidates such as antisense oligonucleotides, aptamers, immunoreceptor-activating nucleotides, or (anti)microRNAs hold great therapeutic promise for many human diseases. Phosphorothioate (PS) backbone modification of nucleotide-based drugs is common practice to protect these promising from rapid degradation by plasma and intracellular nucleases. Effects the changes in physicochemical properties associated with PS on platelets have not been elucidated so far. Here we...
Essentials•Glycoprotein VI (GPVI) binds collagen, starting thrombogenesis, and fibrin, stabilizing thrombi.•GPVI‐dimers, not monomers, recognize immobilized fibrinogen fibrin through their D‐domains.•Collagen, D‐fragment D‐dimer may share a common or proximate binding site(s) on GPVI‐dimer.•GPVI‐dimer–fibrin interaction supports spreading, activation adhesion involving αIIbβ3.Summary: BackgroundPlatelet collagen receptor Glycoprotein initiating stabilizes thrombi by fibrin.ObjectivesTo...
Over recent years, the food industry has striven to reduce waste, mostly because of rising awareness detrimental environmental impacts waste. While edible oils market (mostly represented by soybean oil) is forecasted reach 632 million tons 2022, there increasing interest produce non-soybean, plant-based including, but not limited to, coconut, flaxseed and hemp seed. Expeller pressing organic solvent extractions are common methods for oil extraction in industry. However, these two come with...
The glycoprotein VI (GPVI)-Fc receptor (FcR) gamma-chain complex, a key activatory for collagen on platelet surface membranes, is constitutively associated with the Src family kinases Fyn and Lyn. Molecular cloning of GPVI has revealed presence proline-rich domain in sequence cytoplasmic tail which consensus interaction homology 3 (SH3) domains A series vitro experiments demonstrated ability SH3 both to bind GPVI. Furthermore, depletion (Pro(-)-GPVI) prevented binding Lyn markedly reduced...
Glycoprotein VI (GPVI) is a platelet-specific glycoprotein that has been indicated to react with collagen and activate platelets. Its structure was recently identified by cDNA cloning (Clemetson, J. M., Polgar, J., Magnenat, E., Wells, T. N., Clemetson, K. (1999) Biol. Chem. 274, 29019–29024). However, the mechanism of interaction between GPVI not analyzed in detail because both are insoluble molecules. In this study, we expressed extracellular domain as soluble forms follows: monomeric form...
Platelet interaction with soluble and insoluble collagens was characterized through binding studies. In contrast to resting platelets, cells reacted activators, TS2/16 (integrin α2β1-activating antibody), thrombin, collagen-related peptide, or ADP, exhibited specific collagen that is Mg2+-dependent, but inhibited by prostaglandin I2, Ca2+, Gi9 (anti-integrin α2β1 antibody). Each platelet has 1500–3500 sites, a dissociation constant of 3.5–9 × 10−8m. This the first study show platelets; our...
Abstract This study aimed to determine the effect of pretreating defatted soy flakes with ultrasound on protein isolate (SPI) yield and functionality. Defatted dispersed into water (16%, w/w) were sonicated for 30, 60 120 s at ultrasonic amplitudes 21 84 µm pp (peak peak amplitude in µm), representing low high power, respectively. The power densities 0.30 2.56 W mL −1 , SPI increased by 13 34%, after sonication higher level improved solubility 34% pH 7.0, while decreasing emulsification...
The interaction of platelets with collagen is a complex reaction because monomer does not have any affinity to platelets, but fibrils and immobilized bind strongly platelets. It was postulated that platelet would multiple binding sites for encompass several molecules polymerized each other. mechanism how these or activate still unknown. Two glycoproteins, GP Ia/IIa (integrin alpha 2 beta 1) VI, fulfill the following requirements physiological receptor: antibodies against proteins inhibit...
Evidence was obtained about the mechanism responsible for platelet integrin α2β activation by determining effects of various inhibitors on soluble collagen binding, a parameter to assess α2β1 activation, in stimulated platelets. Agonists that can also activate glycoprotein IIb/IIIa are able α2β1, but those operating via Ib cannot. Activation induced low thrombin or collagen-related peptide concentrations almost completely inhibited apyrase, and wortmannin,...
Abstract Characterization and destabilization of the emulsion formed during aqueous extraction oil from soybean flour were investigated. This was collected as a cream layer subjected to various single combined treatments, including thermal treatments enzymatic aimed at recovery free oil. The processing full fat contains high molecular weight glycinin β‐conglycinin proteins smaller oleosin proteins, which form multilayer interface. Heat treatment alone did not modify but freeze–thaw increased...