Accumulation of the β-amyloid (Aβ) peptide in extracellular senile plaques rich copper and zinc is a defining pathological feature Alzheimer's disease (AD). The Aβ1-x (x=16/28/40/42) peptides have been primary focus Cu(II) binding studies for more than 15 years; however, N-truncated Aβ4-42 major Aβ isoform detected both healthy diseased brains, it contains novel N-terminal FRH sequence. Proteins with His at third position are known to bind avidly, conditional log K values pH 7.4 range...

Aronia fruits contain many valuable components that are beneficial to human health. However, characterized by significant variations in chemical composition dependent on the growing conditions and harvesting period. Therefore, there is a need formulate extracts with precisely defined content of health-promoting substances. dry (ADE) were prepared from frozen pomace applying water extraction, followed purification spray-drying. Subsequently, anthocyanins, phenolic acids, polyphenols was...

Abstract Paper‐based devices are said to be easy make and use. It is true, if you know how. We hope this Review will help people that new the field but also those more experienced who want add some functionalities their systems. Tips common pitfalls provided in each section as well references recommended reading material. Paper have been development for around two millennia, one form or other, ingenious solutions can borrowed from world of paper toys, closer electrochemistry electronics...

PvHCt, a 23-amino acid long, histidine-rich peptide derived from shrimp, becomes strongly antimicrobial upon Cu(ii) ion binding. We describe Zn(ii) and complexes of this peptide, aiming to understand how metal binding structure correlates biological activity. Using NMR, UV-vis, CD FTIR spectroscopies, along with cyclic voltammetry, potentiometry, DFT calculations, we demonstrate that binds the central C-terminal regions inducing significant structural changes. These include pronounced bend...

8-Aminoquinoline (8AQ) is the building block for many medicinal molecules. However, redox properties of 8AQ, which are important understanding stability drugs based on 8AQ in human body, have not been studied detail before. We used cyclic voltammetry and pulse voltammetric techniques to investigate anodic as well cathodic electrochemical behaviour a wide pH range. also applied UV-Vis spectroscopy get better deprotonation due changes. In addition, quantum chemical studies exploring energy...

Copper complexes of metal binding domains synthesized amyloid-β peptides – Aβ(1-16) and N-truncated Aβ(4-16) containing a novel N-terminal FRH sequence, as well its shorter mutants were characterized by cyclic voltammetry. The influence the peptide sequence to copper molar ratio on electrochemical properties obtained structures studied discussed. reversibility redox processes in with Aβ(4-x) derivatives was also investigated. results indicate crucial role Tyr10 process complex, including...

The N-truncated β-amyloid (Aβ) isoform Aβ4–x is known to bind Cu2+ via a redox-silent ATCUN motif with conditional Kd = 30 fM at pH 7.4. This study characterizes the interactions and redox activity of Aβx–16 (x 1, 4) 2-[(dimethylamino)-methyl-8-hydroxyquinoline, terdentate 8-hydroxyquinoline (8HQ) Kd(CuL) 35 pM Metal transfer between Cu(Aβ1–16), CuL, CuL2, ternary CuL(NImAβ) was rapid, while corresponding equilibrium L Aβ4–16 occurred slowly metastable intermediate. Both CuL CuL2 were in...

The Aβ5–x peptides (x = 38, 40, 42) are minor Aβ species in normal brains but elevated upon the application of inhibitors processing enzymes. They interesting from point view coordination chemistry for presence an Arg-His metal binding sequence at their N-terminus capable forming a 3-nitrogen (3N) three-coordinate chelate system. Similar sequences other bioactive were shown to bind Cu(II) ions biological systems. Therefore, we investigated complex formation and reactivity series truncated...

Copper(II) complexes of peptides containing a histidine (His) residue at the third position (known as ATCUN or NTS motif) gain interest for their biological roles and biotechnological applications. We characterized three such peptides, Ala-Ala-His-NH2 (AAH-am), Ala-βAla-His-NH2 (ABH-am) βAla-Ala-His-NH2 (BAH-am) by cyclic voltammetry in order to assess influence substitution α-alanine (Ala) β-alanine (βAla) on redox properties. The obtained results, complementary previously reported...

Abstract Accumulation of the β‐amyloid (Aβ) peptide in extracellular senile plaques rich copper and zinc is a defining pathological feature Alzheimer′s disease (AD). The Aβ1– x ( =16/28/40/42) peptides have been primary focus Cu II binding studies for more than 15 years; however, N‐truncated Aβ4–42 major Aβ isoform detected both healthy diseased brains, it contains novel N‐terminal FRH sequence. Proteins with His at third position are known to bind avidly, conditional log K values pH 7.4...

The necessity to move away from conventional antibiotic therapy has sparked interest in antimicrobial peptides (AMPs). One fascinating example is human CCL-28 chemokine produced by acinar epithelial cells the salivary glands. It can also be released into oral cavity with saliva, playing a crucial role protection. C-terminal domain of possesses antifungal and antibacterial properties, which are likely linked membrane disruption enzyme leakage. Studies suggest that AMPs become more potent...

The influence of cation-π interactions on the electrochemical properties copper(II) complexes with synthesized pentapeptide C-terminal fragment Atrial Natriuretic Factor (ANF) hormone was studied in this work. Molecular modeling performed for Cu(II)-NSFRY-NH2 complex indicated that between Tyr and Cu(II), also Phe-Arg led to specific conformation defined as peptide box, which metal cation is isolated from solvent by ligand. Voltammetry experiments enabled compare redox stability NSFRY-NH2...

Abstract Senile plaques are a hallmark of Alzheimer's disease. The mainly composed small peptides from the β‐amyloid (Aβ) family which differ in how they bind copper ions and thus their properties. pAβ(3‐42) species its Aβ(3‐42) precursor found significant proportion plaques; however, complexes with hardly explored. electrochemical response N‐truncated Aβ(3‐16) pyroglutamate counterpart pAβ(3‐16) was investigated using cyclic voltammetry. Results obtained physiological pH (pH∼7.4) show that...

Alzheimer's disease is the most common neurodegenerative in world and oxidative stress a major factor its pathogenesis. It known that copper(II) ions forming complexes with peptides from β-amyloid (Aβ) group can facilitate production of reactive oxygen species. A very amyloid AD brain plaques Aβ(11–42) form stable Cu(II)-complexes suppress ROS formation. However, when glutamic acid undergoes dehydration cyclic pyroglutamate, resulting new derivative pAβ(11–42), Cu(II) stabilisation much...

Alzheimer's Disease (AD), reported for the first time in 1906, is a common disease that remains incurable to this day. In past, family of treatments using Cu(II) chelators failed during clinical trials, evidencing importance pre-clinical studies. work, we performed complete characterisation TDMQ20, new potential drug against AD, electrochemistry and spectroelectrochemistry. On basis voltammetry, determined TDMQ20 undergoes two-step irreversible oxidation process one-step reduction process....

Abstract Introduction Due to the severe side effects of non-steroidal anti-inflammatory drugs, there is an urgent need find new natural or synthetic chemicals that could act as selective inhibitors cyclooxygenase-2 (COX-2). The most valuable source bioactive compounds and potential drugs are plants. present study aimed at investigation Levidor TM , a patented composition based on oil from seeds Nigella sativa extract root Angelica archangelica, regarding its molecular level. Materials...

The development of Alzheimer’s Disease (AD) has been linked to abnormal quantities β-amyloid peptides in the brain. majority studies have focussed on Aβ(1-40/42) amyloids and their Cu(II)-Aβ(1-40/42) complexes which are responsible for production reactive oxygen nitrogen species (ROS RNS), highly toxic neurons. According recent amyloid plaques, Aβ(4-42), is an N-truncated version Aβ(1-42), as prevalent Aβ(1-42) Although Cu(II) ions, bounded by can be oxidized Cu(III) its do not appear...

Here we demonstrate a significant difference in redox behaviour of copper complexes with β-amyloids Aβ(11-x) and pAβ(11-x) which are models for important components senile plaques. A small change the peptide chain may enhance reactive oxygen species (ROS) formation can severely damage nerve cells.