A5074980576- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Respiratory viral infections research
- Cholesterol and Lipid Metabolism
- Cancer-related molecular mechanisms research
- Viral gastroenteritis research and epidemiology
- Caveolin-1 and cellular processes
- Biosensors and Analytical Detection
- Blood groups and transfusion
- Cancer Immunotherapy and Biomarkers
- Viral Infectious Diseases and Gene Expression in Insects
- Influenza Virus Research Studies
- Renal Transplantation Outcomes and Treatments
- Genomic variations and chromosomal abnormalities
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Steroid Chemistry and Biochemistry
University of Geneva
2024
International Institute of Molecular and Cell Biology
2020
Jagiellonian University
2014-2018
Tulane University
2017-2018
Human coronavirus NL63 (HCoV-NL63) is an alphacoronavirus that was first identified in 2004 the nasopharyngeal aspirate from a 7-month-old patient with respiratory tract infection. Previous studies showed HCoV-NL63 and genetically distant severe acute syndrome (SARS)-CoV employ same receptor for host cell entry, angiotensin-converting enzyme 2 (ACE2), but it largely unclear whether ACE2 interactions are sufficient to allow binding cells. The present study directed expression of (ACE2) on...
ABSTRACT The first steps of human coronavirus NL63 (HCoV-NL63) infection were previously described. virus binds to target cells by use heparan sulfate proteoglycans and interacts with the ACE2 protein. Subsequent events, including internalization trafficking, remain be elucidated. In this study, we mapped process HCoV-NL63 entry into LLC-Mk2 cell line ex vivo three-dimensional (3D) tracheobronchial tissue. Using a variety techniques, have shown that virions require endocytosis for successful...
Virus like particles (VLPs) produced by the expression of viral structural proteins can serve as versatile nanovectors or potential vaccine candidates. In this study we describe for first time generation HCoV-NL63 VLPs using baculovirus system. Major have been expressed in tagged native form, and their assembly to form was evaluated. Additionally, a novel procedure chromatography purification developed. Interestingly, show that these nanoparticles may deliver cargo selectively transduce...
Article13 January 2020Open Access Source DataTransparent process Synthetic lethality between VPS4A and VPS4B triggers an inflammatory response in colorectal cancer Ewelina Szymańska Corresponding Author [email protected] orcid.org/0000-0001-7994-0764 Laboratory of Cell Biology, International Institute Molecular Warsaw, Poland Search for more papers by this author Paulina Nowak Krzysztof Kolmus Magdalena Cybulska Department Genetics, Maria Skłodowska-Curie Institute-Oncology Centre, Goryca...
Human coronavirus (HCoV) NL63 was first described in 2004 and is associated with respiratory tract disease of varying severity. At the genetic structural level, HCoV-NL63 similar to other members Coronavirinae subfamily, especially human 229E (HCoV-229E). Detailed analysis, however, reveals several unique features pathogen. The coronaviral nucleocapsid protein abundantly present infected cells. It a multi-domain, multi-functional important for viral replication number cellular processes. aim...
To maintain plasma membrane (PM) integrity, cells need to acutely regulate PM lipid composition. The Target Of Rapamycin (TOR) complex 2 is a protein kinase that acts as central regulator of homeostasis, but the mechanisms by which it monitors and reacts stresses are poorly understood. address this knowledge gap, we characterized family amphiphilic molecules physically perturb organization in doing so inhibit TORC2 yeast mammalian cells. Using fluorescent associated reporters budding yeast,...