A5000194964- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Respiratory viral infections research
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Cell Image Analysis Techniques
- Respiratory Support and Mechanisms
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Diabetes and associated disorders
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Bacteriophages and microbial interactions
- HIV Research and Treatment
- bioluminescence and chemiluminescence research
- COVID-19 Impact on Reproduction
- vaccines and immunoinformatics approaches
- Biosensors and Analytical Detection
- Insect symbiosis and bacterial influences
- Thermal Regulation in Medicine
- Lipid Membrane Structure and Behavior
Jagiellonian University
2017-2024
ABSTRACT The first steps of human coronavirus NL63 (HCoV-NL63) infection were previously described. virus binds to target cells by use heparan sulfate proteoglycans and interacts with the ACE2 protein. Subsequent events, including internalization trafficking, remain be elucidated. In this study, we mapped process HCoV-NL63 entry into LLC-Mk2 cell line ex vivo three-dimensional (3D) tracheobronchial tissue. Using a variety techniques, have shown that virions require endocytosis for successful...
The Coronaviridae family clusters a number of large RNA viruses, which share several structural and functional features. However, members this recognize different cellular receptors exploit entry routes, what affects their species specificity virulence. aim study was to determine how human coronavirus OC43 enters the susceptible cell. Using confocal microscopy molecular biology tools we visualized early events during infection. We found that virus employs caveolin-1 dependent endocytosis for...
Among seven coronaviruses that infect humans, three (severe acute respiratory syndrome coronavirus [SARS-CoV], Middle East [MERS-CoV], and the newly identified severe 2 [SARS-CoV-2]) are associated with a severe, life-threatening infection multiorgan failure. We previously proposed cationically modified chitosan N-(2-hydroxypropyl)-3-trimethylammonium chloride (HTCC) is potent inhibitor of human NL63 (HCoV-NL63). Next, we demonstrated broad-spectrum antiviral activity compound, as it...
Despite high similarity of canine respiratory coronavirus (CRCoV), bovine coronavirus, (BCoV) and human OC43 (HCoV-OC43), these viruses differ in species specificity. For years it was believed that they share receptor specificity, utilizing sialic acids for cell surface attachment, internalization, entry. Interestingly, careful literature analysis shows indeed bind to the via acids, but there is no solid data moieties mediate virus In our study, using a number techniques, we showed all three...
It is generally accepted that the coronaviral S protein responsible for viral interaction with a cellular receptor. Here we show M also an important player during early stages of HCoV-NL63 infection and concerted action two proteins (M S) prerequisite effective infection. We believe this study broadens understanding biology may alter way in which perceive first steps cell virus. The data presented here be future research into vaccine or drug development.
The COVID-19 pandemic caused by SARS-CoV-2 has been socially and economically devastating. Despite an unprecedented research effort available vaccines, effective therapeutics are still missing to limit severe disease mortality. Using high-throughput screening, we identify acriflavine (ACF) as a potent papain-like protease (PLpro) inhibitor. NMR titrations co-crystal structure confirm that blocks the PLpro catalytic pocket in unexpected binding mode. We show drug inhibits viral replication at...
Currently, there are four seasonal coronaviruses associated with relatively mild respiratory tract disease in humans. However, is also a plethora of animal which have the potential to cross species border. This regularly results emergence new viruses In 2002, severe acute syndrome coronavirus (SARS-CoV) emerged and rapidly disappeared May 2003. 2012, Middle East (MERS-CoV) was identified as possible threat humans, but its pandemic so far minimal, human-to-human transmission ineffective. The...
Natural killer (NK) cells plays a pivotal role in the control of viral infections, and their function depend on balance between activating inhibitory receptors. The immune dysregulation observed COVID-19 patients was previously associated with downregulation NK cell numbers function, yet mechanism inhibition functions interplay infected remain largely unknown. In this study we show that SARS-CoV-2 infection airway epithelial can directly influence phenotype microenvironment. were co-cultured...
ABSTRACT The beginning of 2020 brought us information about the novel coronavirus emerging in China. Rapid research resulted characterization pathogen, which appeared to be a member SARS-like cluster, commonly seen bats. Despite global and local efforts, virus escaped healthcare measures rapidly spread China later globally, officially causing pandemic crisis March 2020. At present, different scenarios are being written contain virus, but development anticoronavirals for all highly pathogenic...
Curcumin, a natural product with recognized antiviral properties, is limited in its application largely due to poor solubility. This study presents the synthesis of water-soluble curcumin-poly(sodium 4-styrenesulfonate) (Cur-PSSNan) covalent conjugates. The antiflaviviral activity conjugates was validated vitro by using Zika virus as model. In development these curcumin-containing derivatives, we used macromolecules reported us also hamper viral infections. Mechanistic investigations...
Virus like particles (VLPs) produced by the expression of viral structural proteins can serve as versatile nanovectors or potential vaccine candidates. In this study we describe for first time generation HCoV-NL63 VLPs using baculovirus system. Major have been expressed in tagged native form, and their assembly to form was evaluated. Additionally, a novel procedure chromatography purification developed. Interestingly, show that these nanoparticles may deliver cargo selectively transduce...
Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus betacoronavirus and close relative human OC43 bovine coronavirus. Here, we examined entry CRCoV into rectal tumor cells (HRT-18G cell line) by analyzing co-localization single particles with cellular markers presence or absence chemical inhibitors pathways potentially involved entry. We also targeted these using siRNA. results show that hijacks caveolin-dependent endocytosis to...
Despite the advances in contemporary medicine and availability of numerous innovative therapies, effective treatment prevention SARS-CoV-2 infections pose a challenge. In search for new anti-SARS-CoV-2 drug candidates, natural products are frequently explored. Here, fifteen cyanopeptolins (CPs) were isolated from Baltic cyanobacterium Nostoc edaphicum tested against SARS-CoV-2. Of these depsipeptides, Arg-containing structural variants showed strongest inhibition Delta infection...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains a major epidemic threat since the beginning of 2020. Efforts to combat virus and associated disease 2019 (COVID-19) are being undertaken worldwide. To facilitate research on itself, number surrogate systems have been developed. Here, we report efficient production SARS-CoV-2 virus-like particles (VLPs) in insect cells. Contrary widely used pseudovirus particles, where only one coronaviral protein is displayed within...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the recently emerged virus responsible for COVID-19 pandemic. Clinical presentation can range from asymptomatic disease and mild tract infection to severe with lung injury, multiorgan failure, death. SARS-CoV-2 third animal emerge in humans 21st century, coronaviruses appear possess a unique ability cross borders between species infect wide of organisms. This somewhat surprising as, except requirement host cell receptors,...
The first line of antiviral immune response in the lungs is secured by innate immunity. Several cell types take part this process, but airway macrophages (AMs) are among most relevant ones. AMs can phagocyte infected cells and activate through antigen presentation cytokine release. However, precise role course SARS-CoV-2 infection still largely unknown. In study, we aimed to evaluate during using a co-culture fully differentiated primary human epithelium (HAE) monocyte-derived (hMDMs). Our...
Abstract SARS-CoV-2 emerged by the end of 2019 to rapidly spread in 2020. At present, it is utmost importance understand virus biology and assess potential existing drugs develop new active compounds. While some animal models for such studies are under development, most research carried out Vero E6 cells. Here, we propose fully differentiated human airway epithelium cultures as a model on SARS-CoV-2. Further, also provide basic characteristics system.
Abstract The Omicron variant of the SARS-CoV-2 virus was first detected in South Africa November 2021. analysis sequence data context earlier variants suggested that it may show very different characteristics, including immune evasion and increased transmission. These assumptions were partially confirmed, reduction protection convalescent patients vaccinated individuals have been confirmed. Here, we evaluated efficacy antivirals against variants, Omicron, Delta, early 2020 isolate.
Zika virus (ZIKV) is an emerging mosquito-borne pathogen associated with microcephaly and other congenital abnormalities in newborns as well neurologic complications adults. The explosive transmission of the last ten years put it limelight improved our understanding its biology pathology. Currently, no vaccine or drugs are available to prevent treat ZIKV infections. Knowing potential flaviviruses broaden their geographic distribution, observed for West Nile virus, importance develop novel...
The battle against emerging viral infections has been uneven, as there is currently no broad-spectrum drug available to contain the spread of novel pathogens throughout population. Consequently, pandemic outbreak that occurred in early 2020 laid bare almost empty state box. Therefore, development treatments with broad specificity become a paramount concern this post-pandemic era. Here, we propose copolymers poly (sodium 2-(acrylamido)-2-methyl-1-propanesulfonate) (PAMPS) and...
This manuscript provides a protocol for in situ hybridization chain reaction (HCR) coupled with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA cell line and three-dimensional (3D) cultures of human airway epithelium. The method allows highly specific sensitive visualization viral by relying on HCR initiated probe localization. Split-initiator probes help amplify the signal fluorescently labeled amplifiers, resulting negligible background...
Even cyanobacteria from ecosystems of low biodiversity, such as the Baltic Sea, can constitute a rich source bioactive metabolites. Potent toxins, enzyme inhibitors, and anticancer antifungal agents were detected in both bloom-forming species less commonly occurring cyanobacteria. In previous work on Pseudanabaena galeata CCNP1313, induction apoptosis breast cancer cell line MCF-7 was documented. Here, activity strain further explored using human dermal fibroblasts, African green monkey...
Abstract Zika virus (ZIKV), belonging to the family of flaviviruses, is an emerging mosquito‐borne pathogen causing microcephaly and other congenital abnormalities in newborns as well various neurologic pathologies adults. The climate change lifestyle current societies, involving global mobility, result a rapid broadening ZIKV geographic distribution. There are no vaccines or drugs available protect against cure diseases caused by that virus. Our vitro studies have demonstrated sodium...