A5006447572- Respiratory viral infections research
- HIV Research and Treatment
- COVID-19 Clinical Research Studies
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Hepatitis B Virus Studies
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Animal Virus Infections Studies
Jagiellonian University
2020-2025
Currently, there are four seasonal coronaviruses associated with relatively mild respiratory tract disease in humans. However, is also a plethora of animal which have the potential to cross species border. This regularly results emergence new viruses In 2002, severe acute syndrome coronavirus (SARS-CoV) emerged and rapidly disappeared May 2003. 2012, Middle East (MERS-CoV) was identified as possible threat humans, but its pandemic so far minimal, human-to-human transmission ineffective. The...
ABSTRACT The Rev-dependent nuclear export of unspliced and singly-spliced transcripts human immunodeficiency virus type 1 (HIV-1) constitutes a critical yet poorly characterized post-transcriptional event essential for effective viral replication. In this study, we engineered dual-fluorescent HIV-1-based cellular reporter system to elucidate the mechanisms underpinning export. By generating multiple stably integrated inducible clones, ensured expression two distinct fluorescent proteins,...
Abstract HIV-1 latency is mainly characterized at transcriptional level, and little known about post-transcriptional mechanisms their contribution to reactivation. The viral protein Rev controls the nucleocytoplasmic export of unspliced singly-spliced RNA that central proviral replication-competence therefore a prerequisite for efficient reactivation during “shock-and-kill” cure therapy. Here we show infection reactivation, subject complex dynamic regulation by cofactor MATR3 MTR4 nuclear...
Abstract Long non-coding RNAs (lncRNAs) constitute the largest and most diverse class of RNAs. They localize to nucleus, cytoplasm, or both compartments, regulate gene expression through various mechanisms at multiple levels. LncRNAs tend evolve faster present higher tissue- developmental stage-specific than protein-coding genes. Initially considered byproducts erroneous transcription without biological function, lncRNAs are now recognized for their involvement in numerous processes, such as...
Abstract SARS-CoV-2 emerged by the end of 2019 to rapidly spread in 2020. At present, it is utmost importance understand virus biology and assess potential existing drugs develop new active compounds. While some animal models for such studies are under development, most research carried out Vero E6 cells. Here, we propose fully differentiated human airway epithelium cultures as a model on SARS-CoV-2. Further, also provide basic characteristics system.
This manuscript provides a protocol for in situ hybridization chain reaction (HCR) coupled with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA cell line and three-dimensional (3D) cultures of human airway epithelium. The method allows highly specific sensitive visualization viral by relying on HCR initiated probe localization. Split-initiator probes help amplify the signal fluorescently labeled amplifiers, resulting negligible background...
This manuscript provides a protocol for in situ hybridization chain reaction (HCR) coupled with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA cell line and three-dimensional (3D) cultures of human airway epithelium. The method allows highly specific sensitive visualization viral by relying on HCR initiated probe localization. Split-initiator probes help amplify the signal fluorescently labeled amplifiers, resulting negligible background...