A5037727639- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Reproductive Health and Technologies
- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Carcinogens and Genotoxicity Assessment
- Mesenchymal stem cell research
- Agriculture, Plant Science, Crop Management
- Renal and related cancers
- Cell death mechanisms and regulation
- Meat and Animal Product Quality
- Bioenergy crop production and management
- Effects and risks of endocrine disrupting chemicals
- Seed Germination and Physiology
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Phagocytosis and Immune Regulation
- Rabbits: Nutrition, Reproduction, Health
- Reproductive System and Pregnancy
- Bee Products Chemical Analysis
- Agriculture and Biological Studies
Jagiellonian University
2019-2024
Malopolska Higher Vocational School of J. Dietl in Kraków
2022
National Research Institute of Animal Production
2009-2021
Hanken School of Economics
2017
Sigma Research (United States)
1999
Olympus (Japan)
1999
Zakład Doświadczalny Instytutu Zootechniki
1991-1998
Ambient air pollution has been associated with a variety of reproductive disorders. However, limited amount research conducted to examine the association between and male outcomes, specifically semen quality.
Significance Lipid droplets (LDs) are stored in the embryo throughout preimplantation development. Yet, role of LDs remains unknown. Embryonic diapause (ED) is a temporary arrest an when it waits for uterine receptivity signal to implant. We provide evidence that play crucial maintaining ED. Diapausing embryos exhibit increased release exosomes reflecting elevated embryonic signaling mother. During ED, decrease lipid caused by switch from carbohydrate metabolism catabolism. have also shown...
The present study was designed to investigate whether environmental exposure pyrethroids associated with sperm DNA damage. Between January 2008 and April 2011 286 men under 45 years of age a normal concentration 15–300 106/ml [WHO 2010] were recruited from an infertility clinic in Lodz, Poland. Participants interviewed provided urine, saliva, semen samples. metabolites: 3-phenoxybenzoic acid (3PBA), cis-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic (CDCCA),...
The relationship between exposure to lifestyle factors and adverse effects on human reproductive health is debated in the scientific literature these controversies have increased public regulatory attention. aim of study was examine association modifiable main semen parameters, sperm morphology, chromatin structure. population consisted 344 men who were attending an infertility clinic for diagnostic purposes with normal concentration 20–300 M/ml or slight oligozoospermia (semen total 15–20...
Diet is a complex exposure variable, which calls for multiple approaches to examine the relationship between diet and disease risk. To address these issues, several authors have recently proposed studying overall dietary patterns by considering how foods nutrients are consumed in combinations. The aim of study was investigate associations patterns, semen quality parameters, level reproductive hormones. population consisted 336 men who attended infertility clinic diagnostic purposes had...
The aim of this study was to evaluate the association between environmental exposure parabens and semen quality parameters [main parameters, computer-aided analysis (CASA parameters], sperm chromatin structure, level reproductive hormones in men [follicle-stimulating hormone (FSH), testosterone, estradiol].Urine samples collected from 315 who attended infertility clinic for diagnostic purposes with normal concentration 15 300 mln/mL were analyzed five concentrations using a validated gas...
Aging | doi:10.18632/aging.203399. Aleksandra Pisarek, Ewelina Pośpiech, Antonia Heidegger, Catarina Xavier, Anna Papież, Danuta Piniewska-Róg, Vivian Kalamara, Ramya Potabattula, Michał Bochenek, Marta Sikora-Polaczek, Aneta Macur, Woźniak, Jarosław Janeczko, Christopher Phillips, Thomas Haaf, Joanna Polańska, Walther Parson, Manfred Kayser, Wojciech Branicki
Natural killer (NK) cells plays a pivotal role in the control of viral infections, and their function depend on balance between activating inhibitory receptors. The immune dysregulation observed COVID-19 patients was previously associated with downregulation NK cell numbers function, yet mechanism inhibition functions interplay infected remain largely unknown. In this study we show that SARS-CoV-2 infection airway epithelial can directly influence phenotype microenvironment. were co-cultured...
Several studies have suggested that human semen quality has declined over past decades and some associated decline with occupational exposures. Many been conducted in settings, where exposure to pollutants is intense. Our objective was examine the association between factors based on an questionnaire, parameters (sperm concentration, motility, sperm morphology) chromatin structure. The study population consisted of 336 men who were attending infertility clinic for diagnostic purposes had a...
Abstract Long non-coding RNAs (lncRNAs) constitute the largest and most diverse class of RNAs. They localize to nucleus, cytoplasm, or both compartments, regulate gene expression through various mechanisms at multiple levels. LncRNAs tend evolve faster present higher tissue- developmental stage-specific than protein-coding genes. Initially considered byproducts erroneous transcription without biological function, lncRNAs are now recognized for their involvement in numerous processes, such as...
Artificial protein cages have potential as programmable, protective carriers of fragile macromolecules to cells. While natural and VLPs been extensively exploited, the use artificial deliver active proteins cells has not yet shown. TRAP-cage is an cage with unusual geometry extremely high stability, which can be triggered break apart in presence cellular reducing agents. Here, we demonstrate that filled a cargo decorated cell-penetrating peptide, allowing it enter Tracking both shows...
Abstract
The unprecedented decline of biodiversity worldwide is urging scientists to collect and store biological material from seriously threatened animals, including large mammals. Lyophilization being explored as a low-cost system for storage in bio-banks cells that might be used expand or restore endangered extinct species through the procedure Somatic Cell Nuclear Transfer (SCNT). Here we report genome intact about 60% lyophylized sheep lymphocytes, whereas DNA damage occurs randomly remaining...
Background and aims: Although psychological stress has been implicated as a cause of idiopathic infertility in both men women, it received little scientific attention among males compared to females. The aim the study was examine association between occupational, life stress, family functioning semen quality.Methods results: population consisted 327 who were attending an clinic for diagnostic purposes. Psychological assessed based on two questionnaires: Subjective Work Characteristics...
In this study, we estimated the distribution of DNA diploidy and aneuploidy in porcine mesenchymal stem cells (pMSCs) that were subjected to osteoblast/osteocyte adipocyte differentiation determine impact long-term vitro culture on cell cycle nuclear profile. This determination could be helpful confirm or exclude suitability physico-chemical conditions for purposes both maintenance an undifferentiated state promote pMSCs. Flow cytometry was applied analyze occurrence aneuploidy/diploidy,...
Abstract Mesenchymal stem cell (MSC) differentiation is regulated intrinsically by transcription factors and extrinsically the extracellular matrix. We tested whether matrix metalloproteinase-2 (MMP-2) its inhibitor TIMP-2, MEF2a TAZ are involved in porcine MSC towards adipocytes osteocytes. Flow cytometry immunofluorescence were used to investigate expression levels of multipotent surface markers CD73 CD105. Real- time PCR was performed detect osteogenic- adipogenic-specific markers,...
Abstract The study was undertaken to examine whether various strategies, including contact inhibition and serum starvation, that were used for artificial synchronization of mitotic cycle porcine fibroblast cell lines affect differently the distribution stage frequencies occurrence apoptotic death in analysed samples. In vitro cultured (contact-inhibited or serumstarved) somatic cells subjected flow cytometric diagnostics together with detection late-apoptotic fractions hypodiploid number...