A5056988673- Effects and risks of endocrine disrupting chemicals
- Toxic Organic Pollutants Impact
- Estrogen and related hormone effects
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Regulation of Appetite and Obesity
- Carcinogens and Genotoxicity Assessment
- Growth Hormone and Insulin-like Growth Factors
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Biochemical Analysis and Sensing Techniques
- Sperm and Testicular Function
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Pharmacological Effects and Toxicity Studies
- Meat and Animal Product Quality
- Per- and polyfluoroalkyl substances research
- Cancer Risks and Factors
- Histone Deacetylase Inhibitors Research
- Ovarian function and disorders
- Animal Behavior and Welfare Studies
- Birth, Development, and Health
- Hypothalamic control of reproductive hormones
- Pregnancy and preeclampsia studies
- Retinoids in leukemia and cellular processes
Jagiellonian University
2011-2020
University of Maryland, Baltimore
1982
Persistent organic pollutants (POPs), such as polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs), biphenyls (PCBs), polybrominated ethers (PBDEs), chloronaftalens (PCNs), bisphenol A (BPA), are stable, lipophilic that affect fertility cause serious reproductive problems, including ovotoxic action, lack of ovulation, premature ovarian failure (POF), or polycystic syndrome (PCOS). Most the representatives POPs influence activation transcription factors, not only aromatic...
Porcine follicular granulosa and theca interna cells were cultured singly in combination. Both cell types isolated from a pool of the same proestrous large follicles. Cultures maintained for 8 days culture medium 199 enriched with 10% calf serum was changed every second day. In half cultures, testosterone, concentration 150 ng/ml (0.5 × 10−7 M), added to medium. The conditioned media subsequently assayed estrogen, progestogen androgen using radioimmunoassay. Unless stated otherwise, data are...
The OVCAR-3 cell line expressing the long (ObRb) and short (ObRt) isoforms of leptin receptor mRNA was used to analyze effect on expression selected genes proteins involved in cycle apoptosis. cells were exposed 2, 20, 40, 100 ng/ml leptin. Cell proliferation determined using alamarBlue viability test flow cytometry. Apoptosis measured a cellular DNA fragmentation ELISA kit. apoptosis evaluated by real-time PCR confirmed western blot. stimulatory action observed as an increase S G2/M phases....
Theca interna cells were collected from large porcine follicles in the follicular phase of cycle. The theca layer was manually separated externa, washed thoroughly, occasionally treated with hyaluronidase solution and trypsinized. resulting cell suspension consisted approximately 70% cells, 8% granulosa 23% nonsteroidogenic cells. In one experiment, same compared. Specific types identified by: 1) staining Oil Red O (ORO) to visualize lipid droplets cytoplasm; 2) measuring activity Δ5,...
Recent animal studies have indicated possible reproductive endocrine effects of levetiracetam (LEV). The aim the present study was to investigate LEV compared valproate (VPA) using human ovarian follicular cells.Cell cultures granulosa cells were prepared. First, effect drugs on basal and follicle-stimulating hormone (FSH)-stimulated estradiol secretion investigated at different concentrations (12, 20, 50, or 80 microg/ml) VPA (75, 100, 250, 350 microg/ml). Second, CYP19 aromatase activity...
Previously, we demonstrated the expression of resistin in porcine ovary, regulation its and direct effect on ovarian steroidogenesis. The objective this study was to examine cell proliferation apoptosis a co-culture model granulosa theca cells. First, analysed at 1 10 ng/ml alone or combination with FSH- IGF1 an alamarBlue assay protein cyclins A B using western blot. Next, mRNA selected pro-apoptotic pro-survival regulators apoptosis, caspase-9, -8 -3 activity DNA fragmentation real time...
Resistin was first reported to be an adipocyte-specific hormone, but recent studies have indicated a connection between resistin and reproductive function. However, it is not yet known if expressed by the ovary can affect steroidogenesis in ovarian follicles from prepubertal pigs. In this study, using real time PCR, immunoblotting, ELISA, we quantified expression concentration maturing (small, 3–4 mm; medium, 4–5 large, 6–7 mm) collected addition, dose-responsive effects of recombinant human...
Monocultures of different placental cells are used for many physiological and toxicological studies; however, they not a true reflection the interaction between placenta fetus. To develop most appropriate model to study endocrine metabolic properties fetoplacental unit we three co-culture models nonfusogenic JEG-3, unsyncytialised BeWo (BeWo) syncytialised (syncBeWo) cultured with adrenal (H295R) cells. As an end point investigated steroids receptors expression steroid secretion, while as...
Vaspin, also known as visceral adipose tissue-derived serine protease inhibitor, is a member of the inhibitor family. Its expression associated with obesity, insulin resistance and type 2 diabetes, elevated concentration observed in polycystic ovary syndrome. However, vaspin has never been studied ovary. Here, we identified two prolific breeds pigs: fat Meishan (MS) lean Large White (LW). We then investigated molecular mechanism involved regulation its response to gonadotropins, insulin,...
Summary: Purpose: Long‐term valproate (VPA) treatment has been associated with hyperandrogenism and polycystic ovaries in women epilepsy. The exact mechanisms of action the drug on sex steroid hormone function are still unsettled. aim present study was to investigate VPA basal gonadotropin‐stimulated secretion porcine ovarian follicular cells measure conversion testosterone estradiol. Second, proliferation apoptosis investigated. Methods: Small medium follicles were obtained from pig days...