A5062175602- Insect and Arachnid Ecology and Behavior
- Fossil Insects in Amber
- Plant and animal studies
- Hemiptera Insect Studies
- Sperm and Testicular Function
- Estrogen and related hormone effects
- Plant Reproductive Biology
- Physiological and biochemical adaptations
- Hormonal and reproductive studies
- Mitochondrial Function and Pathology
- Effects and risks of endocrine disrupting chemicals
- Reproductive Biology and Fertility
- Peroxisome Proliferator-Activated Receptors
- Pregnancy-related medical research
- Developmental Biology and Gene Regulation
- Testicular diseases and treatments
- Neurobiology and Insect Physiology Research
- Marine and coastal plant biology
- Toxic Organic Pollutants Impact
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Botany and Plant Ecology Studies
- Forest Insect Ecology and Management
- Antioxidant Activity and Oxidative Stress
- Per- and polyfluoroalkyl substances research
- Adipokines, Inflammation, and Metabolic Diseases
Jagiellonian University
2014-2024
Ogród Zoologiczny w Krakowie
2014
Oocytes of many invertebrate and vertebrate species contain a characteristic organelle complex known as the Balbiani body (Bb). Until now, three principal functions have been ascribed to this complex: delivery germ cell determinants localized RNAs vegetal cortex/posterior pole oocyte, transport mitochondria towards plasm, participation in formation lipid droplets. Here, we present results computer-aided 3D reconstruction Bb growing oocytes an insect, Thermobia domestica. Our analyses shown...
Abstract In this study, G-coupled estrogen receptor (GPER) was inactivated, by treatment with antagonist (G-15), in testes of C57BL/6 mice: immature (3 weeks old), mature months old) and aged (1.5 years (50 μg/kg bw), as well MA-10 mouse Leydig cells (10 nM/24 h) alone or combination 17β-estradiol antiestrogen (ICI 182,780). G-15-treated mice, overgrowth interstitial tissue found both testes. Depending on age, differences structure distribution various cell organelles were observed....
Telocytes (TCs), a novel type of interstitial cells, are involved in tissue homeostasis maintenance. This study aimed to investigate TC presence the interstitium mouse testis. Additionally, inactivation G-coupled membrane estrogen receptor (GPER) testis was performed obtain insight into function, regulation, and interaction with other cells. Mice were injected GPER antagonist (G-15; 50 μg/kg bw), GPER-signaling effect on distribution, ultrastructure, as well estrogen-related receptors...
Ovarian cancer is characterized by recurrent peritoneal and distant metastasis. To survive in a non-adherent state, floating ovarian spheroids develop mechanisms to resist anoikis. Moreover, ascitic fluid from patients contains high levels of visfatin with anti-apoptotic properties. However, the mechanism which induces anoikis resistance remains unknown. Here, we aimed assess wheather possess properties can induce spheroids.Visfatin synthesis were examined using commercial human ELISA Kit....
We tested whether G-coupled membrane estrogen receptor (GPER) and peroxisome proliferator activated (PPAR) partnership exists this interaction regulates mouse Leydig cell function. Mature aged mice were treated with the antagonist of GPER (G-15; 50 μg/kg b.w). cells (MA-10) G-15 (10 nM) alone or in combination proliferator-activated α γ antagonists, respectively (PPARα, 10 μM; PPARγ, μM). blockage affected testis steroidogenic status via changes lutropin cholesterol levels as well protein...
Oocytes of both vertebrates and invertebrates often contain an intricate organelle assemblage, termed the Balbiani body (Bb). It has previously been suggested that this assemblage is involved in delivery organelles macromolecules to germ plasm, formation oocyte reserve materials, transfer mitochondria next generation. To gain further insight into function Bb, we performed a series analyses experiments, including computer-aided 3-dimensional reconstructions, detection DNA (mtDNA) synthesis as...
Placentation requires the production of numerous growth factors, hormones and transcription factors. Many them, like adipose tissue‑derived leptin or adiponectin, have been identified in placenta their role has established proliferation subsequent development placenta. Apelin is another adipokine known for proliferative effects different cell types. PCR, immunoblotting immunocytochemistry were used to study mRNA protein expression apelin its receptor (APJ) syncytiotrophoblast (BeWo)...
Although epidemiological studies from the last years report an increase in incidences of Leydig cell tumors (previously thought to be a rare disease), biochemical characteristics that tumor important for understanding its etiology, diagnosis, and therapy still remains not completely characterized. Our prior reported G-protein coupled estrogen receptor signaling level disturbances tumors. In addition, we found expressions multi-level-acting lipid balance- steroidogenesis–controlling proteins...
Disruption of granulosa cells (GCs), the main functional in ovary, is associated with impaired female fertility. Epidemiological studies demonstrated that women have detectable levels organic pollutants (e.g., perfluorooctanoate, perfluorooctane sulfonate, 2,2-dichlorodiphenyldichloroethylene, polychlorinated biphenyl 153, and hexachlorobenzene) their follicular fluid (FF), thus these compounds may directly affect function GCs ovary. Considering humans are exposed to multiple simultaneously,...
Abstract Stem cells function in niches, which consist of somatic that control the stem cells' self‐renewal, proliferation, and differentiation. Drosophila ovary germline niche consists terminal filament (TF) cells, cap escort cells; signaling from TF is essential for maintenance (GSCs). Here, we show earwig Opisthocosmia silvestris , female GSC morphologically simple several structurally uniform cells. The most posterior cell (the basal TF) differs remaining separated anterior region...
Three main reproductive strategies have been described among insects: most common oviparity, ovoviviparity and viviparity. In the latter strategy, embryonic development takes place within body of mother which provides gas exchange nutrients for embryos. Here we present results histological EM analyses female system viviparous earwig, Arixenia esau, focusing on all modifications related to We show that in studied species consists two "physiological phases" take clearly disparate compartments,...
To characterize polychlorinated biphenyls (PCBs) action on Leydig cells, PCBs congeners, low-chlorinated (delor 103; d103) and high-chlorinated ones 106; d106) were selected. The cells treated according to dose (d103 or d106 0.2 ng/ml in low doses:, 2 high doses) type + doses 103 106 doses). After 24 h treatment with PCBs, a distinct increase estrogen-related receptors (ERRs α, β γ) expression was revealed. However, the dose- type-dependent effect mostly exerted ERRα expression. A similar...