A5023436858- Xenotransplantation and immune response
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Estrogen and related hormone effects
- Sperm and Testicular Function
- Cancer Cells and Metastasis
- Nerve injury and regeneration
- interferon and immune responses
- Reproductive Physiology in Livestock
- Atrial Fibrillation Management and Outcomes
- Natural product bioactivities and synthesis
- Cardiac Arrhythmias and Treatments
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Cardiac Valve Diseases and Treatments
- NF-κB Signaling Pathways
- Cancer-related molecular mechanisms research
Jagiellonian University
2017-2024
Boldenone (Bdn) and nandrolone (Ndn) are anabolic androgenic steroids (AASs) that, as our previous studies have shown, may increase the risk of neoplastic transformation porcine ovarian putative stem cells (poPSCs). The NF-κB pathway be important in processes carcinogenesis tumour progression. Therefore, this work, we decided to test hypothesis whether Bdn Ndn can activate by acting through membrane androgen receptor ZIP-9. For purpose, expression profiles both genes involved gene coding for...
Abstract In this study, G-coupled estrogen receptor (GPER) was inactivated, by treatment with antagonist (G-15), in testes of C57BL/6 mice: immature (3 weeks old), mature months old) and aged (1.5 years (50 μg/kg bw), as well MA-10 mouse Leydig cells (10 nM/24 h) alone or combination 17β-estradiol antiestrogen (ICI 182,780). G-15-treated mice, overgrowth interstitial tissue found both testes. Depending on age, differences structure distribution various cell organelles were observed....
This study was conducted to explore whether trichostatin A-assisted epigenomic modulation (TSA-EM) can affect the expression of not only recombinant human α1,2-fucosyltransferase (rhα1,2-FT) and α-galactosidase A (rhα-Gal A) immune system enzymes but also Galα1→3Gal epitopes in ex vivo proliferating adult cutaneous fibroblast cells (ACFCs) derived from hFUT2×hGLA bi-transgenic pigs that had been produced for needs future xenotransplantation efforts. The ACFC lines were treated with 50 nM TSA...
Pig-to-human xenotransplantation seems to be the response contemporary shortage of tissue/organ donors. Unfortunately, phylogenetic distance between pig and human implies hyperacute xenograft rejection. In this study, we tested hypothesis that combining expression α1,2-fucosyltransferase (hFUT2) α-galactosidase A (hGLA) genes would allow for removal obstacle in porcine transgenic epidermal keratinocytes (PEKs). We sought determine not only profiles recombinant (rhα1,2-FT) (rhα-Gal A)...
Nandrolone (Ndn) and boldenone (Bdn), the synthetic testosterone analogues with strong anabolic effects, despite being recognized as potentially carcinogenic compounds, are commonly abused by athletes bodybuilders, which includes women, worldwide. This study tested hypothesis that different doses of Ndn Bdn can initiate neoplastic transformation porcine ovarian putative stem cells (poPSCs). Immunomagnetically isolated poPSCs were expanded ex vivo in presence or Bdn, for 7 14 days. Results...
The present study sought to establish the mitotically stable adult cutaneous fibroblast cell (ACFC) lines stemming from hFUT2×hGLA×HLA-E triple-transgenic pigs followed by trichostatin A (TSA)-assisted epigenetically modulating reprogrammability of transgenes permanently incorporated into host genome and subsequent comprehensive analysis molecular signatures related proteomically profiling generated ACFC lines. results Western blot immunofluorescence analyses have proved that profiles...
Contents Mammalian uterus contains a population of mesenchymal stem/progenitor cells that likely contribute to endometrial regeneration during each reproductive cycle. In human and mouse, they reside in perivascular, epithelial stromal compartments the functionalis basalis. Here, we aimed identify tissue resident expressing stem cell markers CD 29, 44, 90, 105, 140b 146 porcine endometrium. We used single immunofluorescence Western blotting. Each these was detected small surrounding blood...
Abstract Endothelial cells (ECs), the primary component of vasculature, play a crucial role in neovascularization. However, number endogenous ECs is inadequate for both experimental purposes and clinical applications. Porcine ovarian putative stem (poPSCs), although not pluripotent, are characterized by great plasticity. Therefore, this study aimed to investigate whether poPSCs have potential differentiate into endothelial lineage. were immunomagnetically isolated from postnatal pig ovaries...
Genetically modified pigs lacking Galα1 → 3Gal and other immunogenic carbohydrates are considered as the most promising, alternative source of various tissues organs for human transplantation. Here, we tested hypothesis that combining expression α1,2-fucosyltransferase (h FUT2 ) α-galactosidase A GLA genes would allow removal this specific carbohydrate in porcine transgenic livers. We investigated profile proteins amount antigen liver single h (n=5), double × (n=5) pigs. Both proteins, A,...
The muscular sleeves (or myocardial extensions) derived from the right ventricle infundibulum myocardium are considered true anatomic substrate for ventricular outflow tract arrhythmias.Pulmonary valve specimens obtained 65 donors (24.6% females, mean age 45.9 ± 15.8 years) were investigated micro-anatomically. Specimens histologically processed, stained with Masson's Trichrome, and examined under a light microscope.The extensions present in left anterior pulmonary sinus 86.2% of cases,...
Abstract Animal models of diabetes, such as db/db mice, are a useful tool for deciphering the genetic background molecular changes at initial stages disease development. Our goal was to find early transcriptomic in three tissues involved metabolism regulation mice: adipose tissue, muscle tissue and liver tissue. Nine animals (three per time point) were studied. Tissues collected 8, 12 16 weeks age. Transcriptome-wide analysis performed using mRNA-seq. Libraries sequenced on NextSeq...
Ograniczona liczba dawców narządów i tkanek do przeszczepów stanowi obecnie poważny problem w transplantologii. Wielu pacjentów co roku umiera niedoczekawszy przeszczepu. Czy istnieje jakaś alternatywa dla ludzkich narządów? Wykorzystanie zwierząt jako komórek, może stanowić odpowiedź na to pytanie. Jednakże wybór optymalnego gatunku jest trudny. Małpy człekokształtne, najbliższe człowiekowi, niestety nie spełniają wielu kryteriów. Ponadto budzą sporo zastrzeżeń etycznych. Świnia gatunek...