A5053403372- Cancer Cells and Metastasis
- Esophageal Cancer Research and Treatment
- Gastrointestinal Tumor Research and Treatment
- 3D Printing in Biomedical Research
- Digestive system and related health
- Gastric Cancer Management and Outcomes
- Esophageal and GI Pathology
- Education and Cultural Studies
- Liver physiology and pathology
- RNA Research and Splicing
- Immune cells in cancer
- Ion Transport and Channel Regulation
- NF-κB Signaling Pathways
- Management and Organizational Practices
- Mesenchymal stem cell research
- Helicobacter pylori-related gastroenterology studies
- Hippo pathway signaling and YAP/TAZ
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- Personality Traits and Psychology
- Macrophage Migration Inhibitory Factor
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Natural product bioactivities and synthesis
- RNA and protein synthesis mechanisms
Jagiellonian University
2011-2024
Klinikum rechts der Isar
2015-2022
Technical University of Munich
2014-2021
Umeå University
2021
University Medical Center Utrecht
2012
The gastrointestinal epithelium is characterized by a high turnover of cells and intestinal stem predominantly reside at the bottom crypts their progeny serve to maintain normal homeostasis. Accumulating evidence demonstrates pivotal role niche surrounding in crypts, which consists cellular soluble components creates an environment constantly influencing fate cells. Here we describe different 3D culture systems that should enable us study cell vitro future: organoid multilayered such as...
Boldenone (Bdn) and nandrolone (Ndn) are anabolic androgenic steroids (AASs) that, as our previous studies have shown, may increase the risk of neoplastic transformation porcine ovarian putative stem cells (poPSCs). The NF-κB pathway be important in processes carcinogenesis tumour progression. Therefore, this work, we decided to test hypothesis whether Bdn Ndn can activate by acting through membrane androgen receptor ZIP-9. For purpose, expression profiles both genes involved gene coding for...
The increasing incidence of esophageal adenocarcinoma (EAC) is mirrored by the prevalence Barrett esophagus, a precursor lesion resulting in large number individuals "at risk" for this lethal malignancy. Among patients with only about 0.3% annually will develop EAC. Because numbers are followed endoscopic surveillance, there need risk prediction among growing population esophagus. We identified four potential biomarkers from an inflammation (IL1β)-dependent mouse model esophagus and tested...
Tumors are composed of the tumor cells and surrounding microenvironment. Both closely interwoven interact by a complex multifaceted cross-talk which plays an integral part in initiation, growth, progression. Dro1/Ccdc80 has been shown to be potent suppressor colorectal cancer ubiquitous inactivation strongly promoted carcinogenesis ApcMin/+ mice chemically-induced model. The aim present study was investigate whether Dro1/Ccdc80's suppressive function is tumor-cell-autonomous. Expression had...
The intestine, together with skin and blood, belongs to the organs highest cell turnover, which makes it a perfect model study cellular processes, such as proliferation differentiation. Epithelial turnover in intestine is possible due presence of intestinal stem cells, are located at bottom crypt. Here, we recapitulate detailed protocol for isolation culture procedures primary epithelial cells three - dimensional (3D) vitro system, described first time by Hans Clevers group (Sato et al.,...
Abstract Recently, three dimensional (3D) organoid cultures were established from different organs; however those are exclusively composed of epithelial cells, thus missing the stromal niche. The later one is believed to play a crucial role during all stages tumor development and resistance therapy. One most abundant important cell in microenvironment myofibroblast. contribution myofibroblasts progression well established, little known about their homeostasis initiation. niche was...
Epithelial-mesenchymal transition (EMT) is a reversible process of epithelial cell transdifferentiation into mesenchymal cell, that enables initiation migration. EMT plays an important role in embryonic development, tissue repair and cancer metastasis. Better understanding cellular molecular events during will not only provide novel insights on how mammalian organism develops tissues regenerate, but also can identify therapeutic targets for therapy. Here we aim to detailed protocol induce...
<p>Supplementary Figure 1 Quantification of each marker (TFF2, DCLK1, GC ratio and LGR5) at a close (<3mm) distal (>3mm) site to the neighboring dysplastic tissue.</p>
<div>Abstract<p>The increasing incidence of esophageal adenocarcinoma (EAC) is mirrored by the prevalence Barrett esophagus, a precursor lesion resulting in large number individuals “at risk” for this lethal malignancy. Among patients with only about 0.3% annually will develop EAC. Because numbers are followed endoscopic surveillance, there need risk prediction among growing population esophagus. We identified four potential biomarkers from an inflammation (IL1β)-dependent mouse...
<div>Abstract<p>The increasing incidence of esophageal adenocarcinoma (EAC) is mirrored by the prevalence Barrett esophagus, a precursor lesion resulting in large number individuals “at risk” for this lethal malignancy. Among patients with only about 0.3% annually will develop EAC. Because numbers are followed endoscopic surveillance, there need risk prediction among growing population esophagus. We identified four potential biomarkers from an inflammation (IL1β)-dependent mouse...
<p>Supplementary Figure 1 Quantification of each marker (TFF2, DCLK1, GC ratio and LGR5) at a close (<3mm) distal (>3mm) site to the neighboring dysplastic tissue.</p>
ABSTRACT Intestinal stromal cells play a key role as the crypt niche during epithelial homeostasis and tumor initiation. However, underlying cellular molecular mechanisms remain unclear. We developed various types of three-dimensional (3D) tissue culture models to small intestinal myofibroblasts (SI MFs) together with enteroids. SI MFs significantly enhanced self-renewal, lumen formation survival enteroids, that was mediated via paracrine mechanism in Wnt-independent manner. Such co-cultured...