A5037731885- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Fungal and yeast genetics research
- Viral Infections and Immunology Research
- Mitochondrial Function and Pathology
- Viral Infectious Diseases and Gene Expression in Insects
- Blood disorders and treatments
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Biofuel production and bioconversion
- Probiotics and Fermented Foods
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Biochemical and Molecular Research
- DNA and Nucleic Acid Chemistry
University of Warsaw
2014-2024
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2013-2024
Polish Academy of Sciences
2004-2014
Human DIS3, the nuclear catalytic subunit of exosome complex, contains exonucleolytic and endonucleolytic active domains. To identify DIS3 targets genome-wide, we combined comprehensive transcriptomic analyses engineered HEK293 cells that expressed mutant with Photoactivatable Ribonucleoside-Enhanced Cross-Linking Immunoprecipitation (PAR-CLIP) experiments. In expressing both sites mutated, RNAs originating from unannotated genomic regions increased ∼2.5-fold, covering ∼70% genome allowing...
The exosome-independent exoribonuclease DIS3L2 is mutated in Perlman syndrome. Here, we used extensive global transcriptomic and targeted biochemical analyses to identify novel substrates human cells. We show that regulates pol II transcripts, comprising selected canonical histone-coding mRNAs, a FTL_short RNA from the ferritin mRNA 5' UTR. Importantly, contributes surveillance of maturing snRNAs during their cytoplasmic processing. Among III particularly targets vault Y RNAs an Alu-like...
Abstract In mammals, m7G-adjacent nucleotides undergo extensive modifications. Ribose of the first or and second transcribed can be subjected to 2′-O-methylation form cap1 cap2, respectively. When nucleotide is 2′-O-methylated adenosine, it additionally modified N6,2′-O-dimethyladenosine (m6Am). Recently, crucial role in distinguishing between ‘self’ ‘non-self’ mammalian cells during viral infection was revealed. Here, we attempted understand impact cap methylations on RNA-related processes....
We report here on the identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase. Immunocytochemical experiments confirm that enzyme indeed localizes to compartment. Inhibition expression by RNA interference results in significant shortening tails ND3, COX III and ATP 6/8 transcripts, suggesting investigated protein represents bona fide This is agreement with our sequencing data which show several messengers are composed almost exclusively adenosine residues. Moreover,...
hDIS3 is a mainly nuclear, catalytic subunit of the human exosome complex, containing exonucleolytic (RNB) and endonucleolytic (PIN) active domains. Mutations in have been found ∼10% patients with multiple myeloma (MM). Here, we show that these mutations interfere activity. Yeast harboring corresponding DIS3 growth inhibition changes nuclear RNA metabolism typical for dysfunction. Construction conditional knockout chicken DT40 cell line revealed essential survival, indicating its function...
The RNA exosome is an essential ribonuclease complex involved in processing and decay. It consists of a 9-subunit catalytically inert ring composed six RNase PH-like proteins forming central channel three cap subunits with KH/S1 domains located at the top. yeast catalytic activity supplied by Dis3 (also known as Rrp44) protein, which has both endo- exoribonucleolytic activities nucleus-specific exonuclease Rrp6. In vitro studies suggest that substrates reach exonucleolytic active site...
Deciphering a function of given protein requires investigating various biological aspects. Usually, the interest is expressed with fusion tag that aids or allows subsequent analyses. Additionally, downregulation inactivation studied gene enables functional studies. Development CRISPR/Cas9 methodology opened many possibilities but in cases it restricted to non-essential genes. Recombinase-dependent integration methods, like Flp-In system, are very good alternatives. The system widely used...
The exosome complex is a major eukaryotic exoribonuclease that requires the SKI for its activity in cytoplasm. In yeast, Ski7 protein links both complexes, whereas functional equivalent of has remained unknown human genome. Proteomic analysis revealed previously uncharacterized short splicing isoform HBS1L (HBS1LV3) long-sought factor linking and complexes humans. contrast, canonical variant, HBS1LV1, which acts as ribosome dissociation factor, does not associate with instead interacts mRNA...
The physiological significance and metabolism of oligoadenylated polyadenylated human mitochondrial mRNAs are not known to date. After study eight transcripts (ND1, ND2, ND3, ND5, CO1, CO2, ATP6/8 Cyt. b) we found a direct correlation between the half-lives their steady-state levels. Investigation mt-mRNA decay after thiamphenicol treatment indicated that three (ND2, ND3 significantly stabilized inhibition translation. Careful analysis one them, showed inaccurate processing H-strand RNA...
Abstract Background Colorectal cancer (CRC) is one of the most commonly diagnosed cancers, posing a serious public health challenge that necessitates development new therapeutics, therapies, and prevention methods. Among various therapeutic approaches, interventions involving lactic acid bacteria (LAB) as probiotics postbiotics have emerged promising candidates for treating preventing CRC. While human-isolated LAB strains are considered highly favorable, those sourced from environmental...
Production of ribosomes relies on more than 200 accessory factors to ensure the proper sequence steps and faultless assembly ribonucleoprotein machinery. Among trans-acting are numerous enzymes, including ribonucleases responsible for processing large rRNA precursor synthesized by RNA polymerase I that encompasses sequences corresponding mature 18S, 5.8S, 25/28S rRNA. In humans, identity most enzymes individual steps, endoribonucleases cleave pre-rRNA at specific sites within regions...
RNA labeling is an invaluable tool for investigation of the function and localization nucleic acids. Labels are commonly incorporated into 3' end primary enzyme used this purpose poly(A) polymerase (PAP), which belongs to class terminal nucleotidyltransferases (NTases). However, PAP preferentially adds ATP analogs, thus limiting number available substrates. Here, we report use another NTase, CutA from fungus Thielavia terrestris. Using enzyme, were able incorporate not only purine but also...