Abstract Background Notch signaling pathway is involved in contact‐dependent communication between the cells of seminiferous epithelium, and its proper activity important for undisturbed spermatogenesis. Objectives The aim was to assess effect inhibition on expression nuclear ( AR ) membrane ZIP 9) androgen receptors androgen‐regulated genes, claudin‐5 claudin‐11, TM 4 mouse Sertoli cell line. Materials methods DAPT (γ‐secretase inhibitor) treatment recombination signal binding protein...

We tested whether G-coupled membrane estrogen receptor (GPER) and peroxisome proliferator activated (PPAR) partnership exists this interaction regulates mouse Leydig cell function. Mature aged mice were treated with the antagonist of GPER (G-15; 50 μg/kg b.w). cells (MA-10) G-15 (10 nM) alone or in combination proliferator-activated α γ antagonists, respectively (PPARα, 10 μM; PPARγ, μM). blockage affected testis steroidogenic status via changes lutropin cholesterol levels as well protein...

Adipokines influence energy metabolism and have effects on male reproduction, including spermatogenesis and/or Sertoli cell maturation; however, the relationship between these active proteins androgens in testicular cells is limited. Here, we studied impact of short-term exposure to flutamide (an anti-androgen that blocks androgen receptors) expression chemerin, apelin, vaspin their receptors (CCRL2, CMKLR1, GPR1, APLNR, GRP78, respectively) adult rat testes. Moreover, levels lipid...

To characterize polychlorinated biphenyls (PCBs) action on Leydig cells, PCBs congeners, low-chlorinated (delor 103; d103) and high-chlorinated ones 106; d106) were selected. The cells treated according to dose (d103 or d106 0.2 ng/ml in low doses:, 2 high doses) type + doses 103 106 doses). After 24 h treatment with PCBs, a distinct increase estrogen-related receptors (ERRs α, β γ) expression was revealed. However, the dose- type-dependent effect mostly exerted ERRα expression. A similar...

Present study was designed to establish a causal connection between changes in the cell-cell junction protein expression at blood-testis barrier and alterations adult rat testis histology following an anti-androgen flutamide exposure. Particular emphasis placed on basal ectoplasmic specialization (ES) seminiferous epithelium of gap protein, connexin 43 (Cx43). Flutamide (50 mg/kg body weight) administered male rats daily from 82 88 postnatal day. Testes 90-day-old control flutamide-exposed...

Abstract Our present knowledge on interrelation between morphology/ultrastructure of mitochondria the Leydig cell and its steroidogenic function is far from satisfactory needs additional studies. Here, we analyzed effects blockade androgen receptor, triggered by exposure to flutamide, expression proteins (1) ultrastructure cells’ constituents (2). We demonstrated that increase in level (StAR, CYP11A1, 3β-HSD, CYP19A1) (and respective mRNAs) rat testicular tissue as well elevation...

Abstract Various animal models are used in the study of alcoholism, with honeybee ( Apis mellifera L.) among them. Here, we tested hypothesis that foragers show higher intoxication resistance to alcohol than nurses, an issue thus far not investigated. To this end, measured latency full sedation when exposed foragers, nurses and reverted nurses. In addition, dehydrogenase (ADH) levels these worker castes. Caste status was confirmed by comparison size their hypopharyngeal glands. We detected...

Our recent study demonstrated altered expression of Notch ligands, receptors, and effector genes in testes pubertal rats following reduced androgen production or signaling. Herein we aimed to explore the role nuclear receptor (AR) membrane (Zrt- Irt-like protein 9; ZIP9) regulation pathway activation rodent Sertoli cells. Experiments were performed using TM4 15P-1 cell lines rat primary cells (PSC). We found that testosterone (10−8 M–10−6 M) increased Notch1 receptor, its active form...

Abstract Background Testis is an immune privileged organ, which prevents the response against sperm antigens and inflammation. Testicular cells responsible for tolerance are mainly Sertoli cells, form blood-testis barrier produce immunosuppressive factors. prevent inflammation in testis maintain by inhibiting proliferation inducing lymphocyte apoptosis. It has been shown that 9-cis-retinoic acid (9cRA) blocks ex vivo apoptosis of peripheral blood lymphocytes promotes differentiation Treg...

Duodenal secretion of HCO3- and luminal release PGE2 were measured in conscious dogs. The results show that the is closely correlated with both outputs increase dose-dependently after topical application hydrochloric acid or arachidonic acid. Indomethacin reduced basal prevented their response to We conclude mucosal plays an important role alkaline from duodenum.

ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA Bodys-Cupak I, Majda A, Grochowska Zalewska-Puchała J, Kamińska Kuzera G. Patient-related stressors and coping strategies in baccalaureate nursing students during clinical practice. Medical Studies/Studia Medyczne. 2019;35(1):41-47. doi:10.5114/ms.2019.84050. APA Bodys-Cupak, I., Majda, A., Grochowska, Zalewska-Puchała, J., Kamińska, & Kuzera, (2019). Medyczne, 35(1), 41-47....

Abstract Background Onset of spermatogenesis at puberty is critically dependent on the activity hypothalamic-pituitary-gonadal axis and testosterone production by Leydig cells. The aim this study was to examine whether activation Notch receptors expression ligands effector genes in rat seminiferous epithelium are controlled androgen signaling during puberty. Methods Peripubertal (5-week-old) Wistar rats received injections flutamide (50 mg/kg bw) daily for 7 days reduce receptor (AR) or a...

Introduction: The rationalization of nursing care can be a direct consequence the low employment rate or unfavorable working environment nurses. Aim: aim study was to learn about factors influencing rationing care. Methods: group consisted 209 nurses in internal medicine departments. used method diagnostic survey, survey technique with use research tools: BERNCA-R questionnaire and PES-NWI (which includes occupational burnout questionnaire). Results: mean total BERNCA score for 1.94 ± 0.75...

Objective Bilirubin is a potent antioxidant, and serum total bilirubin (STB) concentrations correlate negatively with cardiovascular risk. In adult diabetic patients in healthy adults, negative correlation between STB glycated hemoglobin (HbA1c) has been reported. We investigated whether there such an association children adolescents type 1 diabetes mellitus. Methods The study group included 224 duration of more than 12 months. Patients suspected or confirmed hemolytic anemia liver...

Delta/Serrate/LAG-2 (DSL) proteins, which serve as ligands for Notch receptors, mediate direct cell-cell interactions involved in the determination of cell fate and functioning. The present study aimed to explore role androgens estrogens, their receptors regulation DSL proteins Sertoli cells. To this end, primary rat cells TM4 line were treated with either testosterone or 17β-estradiol antagonists receptors. confirm particular knockdown experiments performed. mRNA protein expressions Jagged1...

1. To show hormonal differences between male turkeys with yellow semen syndrome (YSS) and white, normal (WNS), the expression of aromatase, oestrogen receptor α (ERα), β (ERβ) as well testosterone oestradiol concentrations in YSS WNS testes, epididymis, ductus deferens were examined. 2. measure gene levels aromatase receptors (ERs), three complementary techniques (real-time PCR, Western blot, immunohistochemistry) used, whereas steroid hormone determined radio-immunologically. 3....

Abstract Introduction. Migration of professional nurses is a global phenomenon. Polish usually migrate to the relatively richer countries, especially in Europe, thanks opening borders European Union (2004) and legal regulations field education (Directive 2005/36/EC). Aim. The analysis symptoms determinants acculturative stress strategies among migrant working UK. Material methods. study used proprietary questionnaire standardized tool East Asian Acculturation Measure - Scale Acculturative...