A5073432212- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- RNA Interference and Gene Delivery
- Computational Drug Discovery Methods
- Virology and Viral Diseases
- Climate Change and Health Impacts
- HIV Research and Treatment
- Cholinesterase and Neurodegenerative Diseases
- Phytochemicals and Medicinal Plants
- Malaria Research and Control
- Virus-based gene therapy research
- Respiratory viral infections research
- Dental Research and COVID-19
- Insect Pest Control Strategies
- Transplantation: Methods and Outcomes
- Pharmacological Effects of Natural Compounds
- Synthesis and biological activity
- COVID-19 and healthcare impacts
- Biosensors and Analytical Detection
- Retinal and Optic Conditions
- Berberine and alkaloids research
- Machine Learning in Bioinformatics
Jagiellonian University
2019-2024
Among seven coronaviruses that infect humans, three (severe acute respiratory syndrome coronavirus [SARS-CoV], Middle East [MERS-CoV], and the newly identified severe 2 [SARS-CoV-2]) are associated with a severe, life-threatening infection multiorgan failure. We previously proposed cationically modified chitosan N-(2-hydroxypropyl)-3-trimethylammonium chloride (HTCC) is potent inhibitor of human NL63 (HCoV-NL63). Next, we demonstrated broad-spectrum antiviral activity compound, as it...
The development of new antiviral drugs against SARS-CoV-2 is a valuable long-term strategy to protect the global population from COVID-19 pandemic complementary vaccination. Considering this, viral main protease (Mpro) among most promising molecular targets in light its importance during replication cycle. natural flavonoid quercetin 1 has been recently reported be potent Mpro inhibitor vitro, and we explored effect produced by introduction organoselenium functionalities this scaffold. In...
The COVID-19 pandemic caused by SARS-CoV-2 has been socially and economically devastating. Despite an unprecedented research effort available vaccines, effective therapeutics are still missing to limit severe disease mortality. Using high-throughput screening, we identify acriflavine (ACF) as a potent papain-like protease (PLpro) inhibitor. NMR titrations co-crystal structure confirm that blocks the PLpro catalytic pocket in unexpected binding mode. We show drug inhibits viral replication at...
Abstract There are currently no cures for coronavirus infections, making the prevention of infections only course open at present time. The COVID-19 pandemic has been difficult to prevent, as infection is spread by respiratory droplets and thus effective, scalable safe preventive interventions urgently needed. We hypothesise that preventing viral entry into mammalian nasal epithelial cells may be one way limit COVID-19. Here we show...
Curcumin, a natural product with recognized antiviral properties, is limited in its application largely due to poor solubility. This study presents the synthesis of water-soluble curcumin-poly(sodium 4-styrenesulfonate) (Cur-PSSNan) covalent conjugates. The antiflaviviral activity conjugates was validated vitro by using Zika virus as model. In development these curcumin-containing derivatives, we used macromolecules reported us also hamper viral infections. Mechanistic investigations...
Background: Berberine (BBR) is an isoquinoline alkaloid which exhibits a variety of biological and therapeutic properties, has been reported by some to block replication the influenza virus. However, contradictory results have also presented, mechanistic explanation lacking. Methods: A panel cell lines (Madin–Darby canine kidney (MDCK), adenocarcinoma human alveolar basal epithelial cells (A549), lung type I (LET1)) primary airway (HAE) susceptible virus infection were infected with seasonal...
Human metapneumovirus (hMPV) is a widely distributed pathogen responsible for acute upper and lower respiratory infections of varying severity. Previously, we reported that N-sulfonated derivatives poly(allylamine hydrochloride) (NSPAHs) efficiently inhibit replication the influenza virus in vitro ex vivo. Here, show dose dependent inhibition hMPV infection by NSPAHs LLC-MK2 cells. The results showed strong antiviral properties NSPAHs. While activity comparable to those carrageenans, they...
Abstract Clinical data indicate that COVID-19 causes cardiovascular complications, regardless of the severity disease. In this work, we have shown SARS-CoV-2 infection vascular dysfunction due to modification endothelial cell elasticity. We used human pulmonary cells (HPAECs) expressing ACE2 receptor as a model endothelium. This system mimics in vivo conditions, it allows virus entry but not replication. As reference, A549 epithelial cells, well-described supports productive replication...
Abstract There are currently no cures for coronavirus infections, making the prevention of infections only course open at present time. The COVID-19 pandemic has been difficult to prevent, as infection is spread by respiratory droplets and thus effective, scalable safe preventive interventions urgently needed. We hypothesise that preventing viral entry into mammalian nasal epithelial cells may be one way limit COVID-19. Here we show...
COVID-19 caused by SARS-CoV-2 a worldwide crisis, highlighting the importance of preventive measures in infectious diseases control. can remain on surfaces for up to several weeks; therefore, proper disinfection is required mitigate risk indirect virus spreading. Gaseous ozone treatment has received particular attention as an easily accessible tool. In this study, we evaluated virucidal effectiveness gaseous (>7.3 ppm, 2 h) murine hepatitis (MHV)-contaminated stainless-steel surface and...
Abstract The Omicron variant of the SARS-CoV-2 virus was first detected in South Africa November 2021. analysis sequence data context earlier variants suggested that it may show very different characteristics, including immune evasion and increased transmission. These assumptions were partially confirmed, reduction protection convalescent patients vaccinated individuals have been confirmed. Here, we evaluated efficacy antivirals against variants, Omicron, Delta, early 2020 isolate.
A series of anionic homopolymers, poly(sodium 2-(acrylamido)-2-methyl-1-propanesulfonate) (PAMPS) and amphiphilic copolymers AMPS sodium 11-(acrylamido)undecanoate (AaU), both block (PAMPS
Zika virus (ZIKV) is an emerging mosquito-borne pathogen associated with microcephaly and other congenital abnormalities in newborns as well neurologic complications adults. The explosive transmission of the last ten years put it limelight improved our understanding its biology pathology. Currently, no vaccine or drugs are available to prevent treat ZIKV infections. Knowing potential flaviviruses broaden their geographic distribution, observed for West Nile virus, importance develop novel...
The COVID-19 pandemic outbreak prompts an urgent need for efficient therapeutics, and repurposing of known drugs has been extensively used in attempt to get anti-SARS-CoV-2 agents the shortest possible time. glycoside rutin shows manifold pharmacological activities and, despite its use being limited by poor solubility water, it is active principle many pharmaceutical preparations. We herein report our silico experimental investigations as a SARS-CoV-2 Mpro inhibitor water improvement...
A series of poly(ethylene glycol)-block-poly(sodium 4-styrenesulfonate) (PEG-b-PSSNa) copolymers were synthesized, and their antiviral activity against Zika virus (ZIKV) was determined. The polymers inhibit ZIKV replication in vitro mammalian cells at nontoxic concentrations. mechanistic analysis revealed that the PEG-b-PSSNa interact directly with viral particles a zipper-like mechanism, hindering interaction permissive cell. is well-correlated length PSSNa block, indicating copolymers'...
The battle against emerging viral infections has been uneven, as there is currently no broad-spectrum drug available to contain the spread of novel pathogens throughout population. Consequently, pandemic outbreak that occurred in early 2020 laid bare almost empty state box. Therefore, development treatments with broad specificity become a paramount concern this post-pandemic era. Here, we propose copolymers poly (sodium 2-(acrylamido)-2-methyl-1-propanesulfonate) (PAMPS) and...
Abstract Zika virus (ZIKV), belonging to the family of flaviviruses, is an emerging mosquito‐borne pathogen causing microcephaly and other congenital abnormalities in newborns as well various neurologic pathologies adults. The climate change lifestyle current societies, involving global mobility, result a rapid broadening ZIKV geographic distribution. There are no vaccines or drugs available protect against cure diseases caused by that virus. Our vitro studies have demonstrated sodium...
<title>Abstract</title> Clinical data indicate that COVID-19 causes cardiovascular complications, regardless of the severity disease. In this work, we have shown SARS-CoV-2 infection vascular dysfunction due to modification endothelial cell elasticity. We used human pulmonary cells (HPAECs) expressing ACE2 receptor as a model endothelium. This system mimics in vivo conditions, it allows virus entry but not replication. As reference, A549 epithelial cells, well-described supports productive...
S ummary The COVID-19 pandemic caused by SARS-CoV-2 has been socially and economically devastating. Despite an unprecedented research effort, effective therapeutics are still missing to limit severe disease mortality. Using high-throughput screening, we identified acriflavine as a potent papain-like protease (PL pro ) inhibitor. NMR titrations co-crystal structure confirm that blocks the PL catalytic pocket in unexpected binding mode. We show drug inhibits viral replication at nanomolar...
In recent years, the Zika virus (ZIKV) has emerged from a neglected flavivirus to health-threating pathogen that causes malformations and microcephaly in neonates as well neurologic complications adults. ZIKV is transmitted by mosquitoes of Aedes species, which also vector for several other viruses, including yellow fever virus, Chikungunya dengue virus. There neither vaccine nor drugs available prevent or treat infections. Recently, we have demonstrated poly(sodium 4-styrenesulfonate)...
There are currently no cures for coronavirus infections, making the prevention of infections only course open at present time. The COVID-19 pandemic has been difficult to prevent, as infection is spread by respiratory droplets and thus effective, scalable safe preventive interventions urgently needed. We hypothesise that preventing viral entry into mammalian nasal epithelial cells may be one way limit COVID-19. Here we show...