A5054345758- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- HIV Research and Treatment
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Viral Infections and Outbreaks Research
- Advanced Glycation End Products research
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Vibrio bacteria research studies
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Metabolism, Diabetes, and Cancer
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- DNA and Nucleic Acid Chemistry
- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
- Bacterial Genetics and Biotechnology
- Lipid metabolism and biosynthesis
- Hemoglobin structure and function
Universidade Federal do Rio de Janeiro
2016-2025
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2008-2024
Instituto de Biofísica Carlos Chagas Filho
2017-2024
Fundação Carlos Chagas
2017-2019
Pan American Health Organization (Cuba)
2012
National Institute of Science and Technology for Structural Biology and Bioimaging
2011
Scripps Research Institute
2004-2008
Fundación Ciencias Exactas y Naturales
1999
University of Buenos Aires
1999
Universidad de Granada
1999
Dengue virus (DENV) affects millions of people, causing more than 20,000 deaths annually. No effective treatment for the disease caused by DENV infection is currently available, partially due to lack knowledge on basic aspects viral life cycle, including molecular basis interaction between components and cellular compartments. Here, we characterized properties capsid (C) protein hepatic lipid droplets (LDs), which was recently shown be essential replication cycle. Zeta potential analysis...
Abstract Hyperglycemia is a common feature of diabetes mellitus, considered as risk factor for cancer. However, its direct effects in cancer cell behavior are relatively unexplored. Herein we show that high glucose concentration induces aberrant glycosylation, increased proliferation, invasion and tumor progression colon By modulating the activity rate-limiting enzyme, glutamine-fructose-6-phosphate amidotransferase (GFAT), demonstrate hexosamine biosynthetic pathway (HBP) involved those...
Dengue is the major arthropod-borne human viral disease, for which no vaccine or specific treatment available. We used NMR, zeta potential measurements and atomic force microscopy to study structural features of interaction between dengue virus C (capsid) protein LDs (lipid droplets), organelles crucial infectious particle formation. protein-binding sites LD were mapped, revealing a new function conserved segment in N-terminal disordered region indicating that conformational selection...
Dengue is one of the main public health concerns worldwide. Recent estimates indicate that over 390 million people are infected annually with dengue virus (DENV), resulting in thousands deaths. Among DENV nonstructural proteins, NS1 protein only whose function during replication still unknown. a 46- to 55-kDa glycoprotein commonly found as both membrane-associated homodimer and soluble hexameric barrel-shaped lipoprotein. Despite its role pathogenic process, essential for proper RNA...
ABSTRACT Dengue virus (DENV) infects millions of people worldwide and is a major public health problem. DENV nonstructural protein 1 (NS1) conserved glycoprotein that associates with membranes also secreted into the plasma in DENV-infected patients. The present study describes novel mechanism by which NS1 inhibits terminal complement pathway. We first identified regulator vitronectin (VN) as DENV2 binding partner using yeast two-hybrid system. This interaction was further assessed...
Diabetes Mellitus (DM) is a group of chronic metabolic diseases distinguished by elevated glycemia due to the alterations in insulin metabolism. DM one most relevant modern world, with high incidence and prevalence worldwide, associated severe systemic complications increased morbidity mortality rates. Although genetic factors lifestyle habits are two main involved onset, viral infections, such as enteroviruses, cytomegalovirus, hepatitis C virus, human immunodeficiency acute respiratory...
Products of the umuD gene in Escherichia coli play key roles coordinating switch from accurate DNA repair to mutagenic translesion synthesis (TLS) during SOS response damage. Homodimeric UmuD 2 is up-regulated 10-fold immediately after damage, which slow autocleavage removes N-terminal 24 amino acids each UmuD. The remaining fragment, UmuD′ , required for TLS. small proteins and make a large number specific protein–protein contacts, including three five known E. polymerases, parts...
Dengue virus (DENV) represents a major threat to public health worldwide. Early DENV diagnosis should not only detect the infection but also identify patients with higher likelihood develop severe cases. Previous studies have suggested potential for NS1 serve as viral marker dengue severity. However, further using different sera panels are required confirm this hypothesis. In context, we developed lab-based ELISA and quantitate protein from four serotypes primary secondary This approach was...
The incidence of flavivirus infections has increased dramatically in recent decades tropical and sub-tropical climates worldwide, affecting hundreds millions people each year. Flaviviridae family includes dengue, West Nile, Zika, Japanese encephalitis, yellow fever viruses that are typically transmitted by mosquitoes or ticks, cause a wide range symptoms, such as fever, shock, meningitis, paralysis, birth defects, death. genome is composed single positive-sense RNA molecule encoding viral...
Emerging evidence identifies major contributions of platelets to inflammatory amplification in dengue, but the mechanisms infection-driven platelet activation are not completely understood. Dengue virus nonstructural protein-1 (DENV NS1) is a viral protein secreted by infected cells with recognized roles dengue pathogenesis, it remains unknown whether NS1 contributes phenotype platelets. This study shows that recombinant DENV activated toward an partially reproduced infection. stimulation...
DENV is one of the most relevant mosquito-transmitted viruses worldwide, infecting more than 390 million people every year and leading to 20 thousand deaths. Although a vaccine has already been approved, its potential side effects have hampered use in large-scale immunizations.
Introduction The dengue virus (DENV) is the etiological agent that causes fever illness, an arbovirus with a major endemic potential has become increasingly prevalent in Brazil and already been associated fatal cases children. DENV tropism for several organs, including lungs causing pulmonary complications. aim of this article was to evaluate inflammatory histopathological profile lung tissue three children infected DENV, which represents group more susceptible fatality due its incomplete...
Cooperativity in the interactions among proteins subunits and DNA is crucial for recognition. LexA repressor was originally thought to bind as a monomer, with cooperativity leading tighter binding of second monomer. The main support this model high value dissociation constant dimer (micromolar range). Here we show that protein at nanomolar concentrations under different conditions. reversible investigated by effects hydrostatic pressure or urea, using fluorescence emission polarization...
Dengue constitutes a global health concern. The clinical manifestation of this disease varies from mild febrile illness to severe hemorrhage and/or fatal hypovolemic shock. Flavivirus nonstructural protein 1 (NS1) is secreted glycoprotein that displayed on the surface infected cells but absent in viral particles. NS1 accumulates at high levels plasma dengue virus (DENV)-infected patients, and previous reports highlight its involvement immune evasion, severity, liver dysfunction pathogenesis....
Dengue is an important mosquito-borne disease and a global public health problem. The caused by dengue virus (DENV), which member of the Flaviviridae family contains positive single-stranded RNA genome that encodes single precursor polyprotein further cleaved into structural non-structural proteins. Among these proteins, 3 (NS3) protein very because it forms non-covalent complex with NS2B cofactor, thereby forming functional viral protease. NS3 also C-terminal ATPase/helicase domain...
The cestodes constitute important but understudied human and veterinary parasites. Their surfaces are rich in carbohydrates, on which very little structural information is available. tissue-dwelling larva (hydatid cyst) of the cestode Echinococcus granulosus outwardly protected by a massive layer carbohydrate-rich extracellular matrix, termed laminated layer. monosaccharide composition this suggests that its major carbohydrate components exclusively mucin-type O-glycans. We have purified...
The dengue non-structural 3 (NS3) is a multifunctional protein, containing serino-protease domain, located at the N-terminal portion, and helicase, NTPase RTPase domains present in C-terminal region. This protein considered main target for CD4+ CD8+ T cell responses during infection, which may be involved protection. However, few studies have been undertaken evaluating use of this as protective antigen against dengue, well other flavivirus. In work, we investigate efficacy DNA vaccines based...
High mobility group box (HMGB) proteins are abundant nonhistone found in all eukaryotic nuclei and capable of binding/bending DNA. The human HMGB1 is composed two binding motifs, known as Boxes A B, L-shaped alpha-helix structures, followed by a random-coil acidic tail that consists 30 Asp Glu residues. This work aimed at evaluating the role protein stability DNA interactions. For this purpose, we cloned, expressed purified its tailless form, HMGB1ΔC, E. coli strain. Tryptophan fluorescence...
Supercharged proteins are a recently identified class of that have the ability to efficiently deliver functional macromolecules into mammalian cells. They were first developed as bioengineering products, but later found in human proteome. In this work, we show with unusually high net positive charge is frequently among viral structural proteins, more specifically capsid proteins. particular, viruses from Flaviviridae family all very molecular weight ratio (> +1.07/kDa), thus qualifying...