A5038598317- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Immunotherapy and Immune Responses
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Neonatal and fetal brain pathology
- Poxvirus research and outbreaks
- Traumatic Brain Injury and Neurovascular Disturbances
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Metabolism and Genetic Disorders
- Bacillus and Francisella bacterial research
- Immune responses and vaccinations
- S100 Proteins and Annexins
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Retinal Diseases and Treatments
- Cardiovascular Disease and Adiposity
- Complement system in diseases
- SARS-CoV-2 detection and testing
- Biomedical Research and Pathophysiology
Universidade Federal do Rio de Janeiro
2014-2025
Yale University
2024
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2023
Escola Bahiana de Medicina e Saúde Pública
2023
Universidade Federal do Espírito Santo
2020
Fundação Oswaldo Cruz
2013
Abstract The SARS-CoV-2 pandemic highlighted the urgent need for innovative methods to study humoral immune responses and identify monoclonal antibodies (mAbs) with diagnostic therapeutic potential. Memory B cells (MBCs), pivotal adaptive immunity, generate high-affinity upon antigen re-encounter. While single-cell high-throughput sequencing has revolutionized antibody repertoire studies, it critical limitations: inability simultaneously determine antigen-binding specificities immunoglobulin...
Abstract Immunological memory mediates rapid protection following infection or vaccination including heterologous exposure. However, cross-reactive responses in humans remain poorly characterized. We explored the longevity and specificity of cross-protective to orthopoxviruses through smallpox Mpox virus (MPXV) infection. Smallpox using Vaccinia (VACV)-based vaccines provides a unique opportunity study long-term immunity without antigen re-exposure. assessed systemic mucosal four human...
Besides antigen-specific responses to viral antigens, humoral immune response in virus infection can generate polyreactive and autoreactive antibodies. Dengue Zika infections have been linked antibody-mediated autoimmune disorders, including Guillain-Barré syndrome. A unique feature of flaviviruses is the secretion nonstructural protein 1 (NS1) by infected cells. NS1 highly immunogenic, antibodies targeting both protective pathogenic roles. In present study, we investigated found be an...
Background The Trypanosoma cruzi infection is associated with severe T cell unresponsiveness to antigens and mitogens characterized by decreased IL-2 synthesis. mucin (Tc Muc) has been implicated in this phenomenom. These molecules contain a unique type of glycosylation consisting several sialylated O-glycans linked the protein backbone via N-acetylglucosamine residues. Methodology/Principal Findings In study, we evaluated ability Tc Muc modulate activation CD4+ cells. Our data show that...
Zika virus (ZIKV) disease has become a global health emergency with devastating effects on public health. Recent evidences implicate the as an emergent neuropathological agent promoting serious pathologies of human nervous system, that include destructive and malformation consequences such development ocular fetal brain lesions, microcephaly in neonates, Guillain-Barré syndrome (GBS) adults. These neurological disorders both central peripheral systems are thought to be associated neurotropic...
Cardiovascular comorbidities and immune-response dysregulation are associated with COVID-19 severity. We aimed to explore the key immune cell profile understand its association disease progression in 156 patients hypertension that were hospitalized due COVID-19. The primary outcome was severe disease. probability of estimated using a logistic regression model included clinical variables subsets outcome. Obesity; diabetes; oxygen saturation; lung involvement on computed tomography (CT)...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to be a global problem in part because of the emergence variants concern that evade neutralization by antibodies elicited prior infection or vaccination. Here we report on human neutralizing antibody and memory responses Gamma variant cohort hospitalized individuals. Plasma from infected individuals potently neutralized viruses pseudotyped with SARS-CoV-2 spike protein, but activity against Wuhan-Hu-1-1, Beta, Delta,...
<title>Abstract</title> Immunological memory mediates rapid protection following infection or vaccination including heterologous exposure. However, cross-reactive responses in humans remain poorly characterized. We explored the longevity and specificity of cross-protective to orthopoxviruses through smallpox Mpox virus (MPXV) infection. Smallpox using Vaccinia (VACV)-based vaccines provides a unique opportunity study long-term immunity without antigen re-exposure. assessed systemic mucosal...
Abstract Although brain scars in adults have been extensively studied, there is less data available regarding scar formation during the neonatal period, and involvement of peripheral immune cells this process remains unexplored neonates. Using a murine model hypoxic–ischemic encephalopathy (HIE) confocal microscopy, we characterized scarring examined recruitment to cortical hippocampal for up 1 year post‐insult. Regional differences were observed, including presence reticular fibrotic...
Multiple myeloma (MM) is a plasma cell disorder that still remains incurable. The immune dysfunction of the host striking characteristic MM, leading to tumor growth and reducing survival rate patients. Monocytes are precursors conventional dendritic cells (DCs), major player in immunity mechanisms driving protective T responses against tumor. Herein, we report human MM RPMI 8226 line shows pronounced chemoattractant activity for monocytes also expresses enhanced levels leukocyte chemotactic...
The SARS-CoV-2 pandemic has had a social and economic impact worldwide, vaccination is an efficient strategy for diminishing those damages. New adjuvant formulations are required the high vaccine demands, especially that induce Th1 phenotype. Herein we assess using combination of Alum polyinosinic:polycytidylic acid [Poly(I:C)] adjuvants plus spike protein in prefusion trimeric conformation by intradermal (ID) route. We found levels IgG anti-spike antibodies serum enzyme linked immunosorbent...
Cardiac involvement in 2019 coronavirus disease (COVID-19) survivors has been reported frequently. An exacerbated immune response may be the main mechanism of myocardial injury and late cardiac sequelae this population.
Antibody-based therapy depletes myeloid-biased hematopoietic stem cells (my-HSCs) to rejuvenate the immune system and improve responses in aged mice.
Cerebral malaria (CM) is a clinical syndrome involving irreversible and lethal signs of brain injury associated to infection by parasites the genus Plasmodium. The pathogenesis CM derives from infection-induced proinflammatory cytokines with cytoadherence parasitized red blood cells microvasculature. Glycoconjugates are very abundant in surface Plasmodium spp., critical mediators parasite virulence host-pathogen interactions. Herein, we show that 6-Diazo-5-oxo-L-norleucine (DON)...
Despite the advances in treating neonatal hypoxic-ischemic encephalopathy (HIE) with induced hypothermia, rates of severe disability are still high among survivors. Preclinical studies have indicated that cell therapies hematopoietic stem/progenitor cells could improve neurological outcomes HIE. In this study, we investigated whether administration AMD3100, a CXCR4 antagonist mobilizes into circulation, has therapeutic effects HIE.P10 Wistar rats both sexes were subjected to right common...
Abstract The SARS-CoV-2 pandemic has had a social and economic impact worldwide, vaccination is an efficient strategy for diminishing those damages. New adjuvant formulations are required the high vaccine demands, especially that induce Th1 phenotype. Herein we assess using combination of Alum polyinosinic:polycytidylic acid (Poly(I:C)) adjuvants plus spike protein in prefusion trimeric conformation by intradermal (ID) route. We found levels IgG anti-spike antibodies serum enzyme linked...
The immune system consists in part of a functionally competent T -cell repertoire that is reactive to foreign antigens but tolerant self -antigens. cells primarily formed the thymusthrough positive and negative selection developing thymocytes are critical for establishing central tolerance. One features thymus sense stress hormones produced pathophysiological conditions. Increased levels these associated with infections able induce thymic atrophy. We have shown acuteTrypanosoma...