A5006730840- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- Rabies epidemiology and control
- Biosensors and Analytical Detection
- COVID-19 epidemiological studies
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- vaccines and immunoinformatics approaches
- Toxoplasma gondii Research Studies
- Streptococcal Infections and Treatments
- Influenza Virus Research Studies
- Virology and Viral Diseases
Universidade Federal do Rio de Janeiro
2020-2022
Instituto de Agrobiotecnología del Litoral
2020
We used the recombinant trimeric spike (S) glycoprotein in prefusion conformation to immunize horses for production of hyperimmune globulins against SARS-CoV-2. Serum antibody titers measured by ELISA were above 1:10
Background: Accurate serological tests are essential tools to allow adequate monitoring and control of COVID-19 spread. Production a low-cost high-quality recombinant viral antigen can enable the development reliable affordable assays, which urgently needed facilitate epidemiological surveillance studies in low-income economies.Methods: Trimeric SARS-COV-2 spike (S) protein was produced serum-free, suspension-adapted HEK293 cells. Highly purified S used develop an ELISA, named S-UFRJ test....
Abstract Accurate serological tests are essential tools to allow adequate monitoring and control of COVID-19 spread. Production a low-cost high-quality recombinant viral antigen can enable the development reliable affordable assays, which urgently needed facilitate epidemiological surveillance studies in low-income economies. Trimeric SARS-COV-2 spike (S) protein was produced serum-free, suspension-adapted HEK293 cells. Highly purified S used develop an ELISA, named S-UFRJ test. It...
Abstract We used the trimeric spike (S) glycoprotein (residues 1-1208) in prefusion conformation to immunize horses for production of hyperimmune globulins against SARS-CoV-2. Serum antibody titers measured by anti-spike ELISA were above 1:1,000,000, and neutralizing titer was 1:14,604 (average PRNT 90 ), which is 140-fold higher than average plasma from three convalescent COVID-19 patients analyzed comparison. Using same technology routinely industrial other horse products, immunized...
The severe acute respiratory syndrome spread worldwide, causing a pandemic. SARS-CoV-2 mutations have arisen in the spike, glycoprotein at viral envelope and an antigenic candidate for vaccines against COVID-19. Here, we present comparative data of glycosylated full-length ancestral D614G spike together with three other transmissible strains classified by World Health Organization as variants concern: beta, gamma, delta. By showing that has less hydrophobic surface exposure trimer...
Despite the intramuscular route being most used vaccination strategy against SARS-CoV-2, intradermal has been studied around globe as a strong candidate for immunization SARS-CoV-2. Adjuvants have shown to be essential vaccine components that are capable of driving robust immune responses and increasing efficacy. In this work, our group aimed develop SARS-CoV-2 using trimeric spike protein, by testing best with formulations containing adjuvants AddaS03, CpG, MPL, Alum, or combination two...
The SARS-CoV-2 pandemic has had a social and economic impact worldwide, vaccination is an efficient strategy for diminishing those damages. New adjuvant formulations are required the high vaccine demands, especially that induce Th1 phenotype. Herein we assess using combination of Alum polyinosinic:polycytidylic acid [Poly(I:C)] adjuvants plus spike protein in prefusion trimeric conformation by intradermal (ID) route. We found levels IgG anti-spike antibodies serum enzyme linked immunosorbent...
Abstract Accurate serological tests are essential tools to allow adequate monitoring and control of COVID-19 spread. Production a low-cost high-quality recombinant viral antigen can enable the development reliable affordable assays, which urgently needed facilitate epidemiological surveillance studies in low-income economies. Trimeric SARS-COV-2 spike (S) protein was produced serum-free, suspension-adapted HEK293 cells. Highly purified S used develop an ELISA, named S-UFRJ test. It...
Abstract The SARS-CoV-2 pandemic has had a social and economic impact worldwide, vaccination is an efficient strategy for diminishing those damages. New adjuvant formulations are required the high vaccine demands, especially that induce Th1 phenotype. Herein we assess using combination of Alum polyinosinic:polycytidylic acid (Poly(I:C)) adjuvants plus spike protein in prefusion trimeric conformation by intradermal (ID) route. We found levels IgG anti-spike antibodies serum enzyme linked...
Abstract The severe acute respiratory syndrome CoV-2 rapidly spread worldwide, causing a pandemic. After period of evolutionary stasis, set SARS-CoV-2 mutations has arisen in the spike, leading glycoprotein at viral envelope and primary antigenic candidate for vaccines against 2019 CoV disease (COVID-19). Here, we present comparative biochemical data glycosylated full-length ancestral D614G spike together with three other highly transmissible strains classified by World Health Organization...
We used the trimeric spike (S) glycoprotein (residues 1-1208) in prefusion conformation to immunize horses for production of hyperimmune globulins against SARS-CoV-2. Serum antibody titers measured by anti-spike ELISA were above 1:106, and neutralizing titer an early isolate authentic virus (WT) was 1:14,604 (average PRNT90), which is 140-fold higher than average plasma from three convalescent COVID-19 patients analyzed comparison. Using same technology routinely industrial other horse...