A5035029856- Ovarian cancer diagnosis and treatment
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Galectins and Cancer Biology
- BRCA gene mutations in cancer
- Glycosylation and Glycoproteins Research
- Molecular Biology Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Cancer-related Molecular Pathways
- Bioinformatics and Genomic Networks
- Drug Transport and Resistance Mechanisms
- Pharmacogenetics and Drug Metabolism
- Monoclonal and Polyclonal Antibodies Research
- DNA Repair Mechanisms
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Endoplasmic Reticulum Stress and Disease
- Reflective Practices in Education
- Cancer, Hypoxia, and Metabolism
- Toxin Mechanisms and Immunotoxins
- Genetics, Bioinformatics, and Biomedical Research
Saint Joseph's College of Maine
2024
University of Allahabad
2024
Universidade Federal do Rio de Janeiro
2014-2024
SUNY New Paltz
2022
Fundação Oswaldo Cruz
2010-2022
Pharmaceutical Biotechnology (Czechia)
2020
Moffitt Cancer Center
2013-2018
Centro Universitário FIEO
2018
Biotech (Brazil)
2018
Instituto Nacional do Câncer
2008-2015
Cancer is one of the biggest problems in public health worldwide. Plants have been shown important role anticancer research. Viscum album L. (Santalaceae), commonly known as mistletoe, a semi-parasitic plant that grows on different host trees. In complementary medicine, extracts from European mistletoe (Viscum L.) used treatment cancer. The study was conducted to identify chemical composition and antitumor potential tinctures. Chemical analysis performed by high resolution chromatography...
Prion diseases are characterized by prion protein (PrP) transmissible aggregation and neurodegeneration, which has been linked to oxidative stress. The physiological function of PrP seems related sequestering redox-active Cu 2+ , dyshomeostasis is observed in disease brain. It unclear whether contributes aggregation, recently shown be mediated condensation. This study indicates that promotes condensation live cells at the cell surface vitro through copartitioning. Molecularly, inhibited...
<h3>Background</h3> Inactivating germline mutations in the tumour suppressor gene <i>BRCA1</i> are associated with a significantly increased risk of developing breast and ovarian cancer. A large number (>1500) unique variants have been identified population can be classified as pathogenic, non-pathogenic or unknown significance (VUS). Many VUS rare missense leading to single amino acid changes. Their impact on protein function cannot directly inferred from sequence information, precluding...
Interethnic admixture is a source of cryptic population structure that may lead to spurious genotype-phenotype associations in pharmacogenomic studies. We studied the impact stratification on distribution ABCB1 polymorphisms (1236C>T, 2677G>T/A and 3435C>T) among Brazilians, highly admixed with Amerindian, European African ancestral roots.Individual DNA from 320 healthy adults was genotyped panel ancestry informative markers, proportions component (ACA) were estimated. genotypes determined...
DNA damage repair (DDR) is an orchestrated process encompassing the injury detection to its complete resolution. double-strand break lesions are repaired mainly by two distinct mechanisms: error-free homologous recombination (HR) and error-prone non-homologous end-joining. Galectin-3 (GAL3) unique member of chimeric galectins subfamily reported be involved in several cancer development progression related events. Recently our group described a putative protein interaction between GAL3 BARD1,...
Abstract Genome-wide association studies have identified 40 ovarian cancer risk loci. However, the mechanisms underlying these associations remain elusive. In this study, we conducted a two-pronged approach to identify candidate causal SNPs and assess biological at chromosome 9p22.2, first most statistically significant associated locus for susceptibility. Three transcriptional regulatory elements with allele-specific effects scaffold/matrix attachment region were characterized and, through...
Background: Accurate serological tests are essential tools to allow adequate monitoring and control of COVID-19 spread. Production a low-cost high-quality recombinant viral antigen can enable the development reliable affordable assays, which urgently needed facilitate epidemiological surveillance studies in low-income economies.Methods: Trimeric SARS-COV-2 spike (S) protein was produced serum-free, suspension-adapted HEK293 cells. Highly purified S used develop an ELISA, named S-UFRJ test....
Abstract Accurate serological tests are essential tools to allow adequate monitoring and control of COVID-19 spread. Production a low-cost high-quality recombinant viral antigen can enable the development reliable affordable assays, which urgently needed facilitate epidemiological surveillance studies in low-income economies. Trimeric SARS-COV-2 spike (S) protein was produced serum-free, suspension-adapted HEK293 cells. Highly purified S used develop an ELISA, named S-UFRJ test. It...
Resistance training (RT) is performed at distinct levels of intensity from the beginning to end exercise sets, increasing sensation effort as progress more vigorous levels, commonly leading changes on facial expression RT practitioners. The objective this study evaluate in expressions using Facial Action Coding System(FACS) and activation muscles by surface electromyography(sEMG) two different during resistance investigate correlation between fatigue. Eleven healthy male participants...
Double strand break lesions, the most toxic type of DNA damage, are repaired primarily through 2 distinct pathways: homology-directed recombination (HR) and non-homologous end-joining (NHEJ). BRCA1 53BP1, proteins containing BRCT modular domain, play an important role in damage response (DDR) by orchestrating decision between HR NHEJ, but precise mechanisms regarding both pathways not entirely understood. Previously, our group identified a putative interaction BARD1 (BRCA1-associated RING...
The mechanism of immunosuppression induced by severe sepsis is not fully understood. production prostaglandin E2 (PGE2) during well known, but its role in long-term consequences has been explored. current study evaluates the PGE2 development secondary to and potential as therapeutic target. Cecal ligation puncture was used an experimental model for induction Balb/c C57BL/6 mice. Immunosuppression evaluated response infection with Aspergillus fumigatus survivors. prostanoids vivo vitro...
Extracellular vesicles, such as microvesicles (MVs), were identified important players in tumor progression and acquisition of an aggressive phenotype. Tissue factor (TF) is a transmembrane protein that initiates the blood coagulation cascade. In cells, TF has been associated with aggressiveness cancer progression. Previous studies demonstrate incorporated into MVs secreted by cells; however, it unknown whether actively involved release MVs. Here, we investigated influence expression on...
Abstract Prion diseases are characterized by prion protein (PrP) transmissible aggregation and toxicity in the brain. The physiological function of PrP seems related to sequestering internalization redox-active Cu 2+ . It is unclear whether contributes aggregation, recently shown be mediated condensation. We investigated role oxidation condensation using multiple biophysical biochemical methods. find that promotes at cell surface vitro through co-partitioning. Molecularly, inhibited...
It has been reported that malaria reduces cytochrome-P450 (CYP) content and monooxygenase activities in the mammalian host liver. The mechanism by which modulates CYP activities, however, remains unclear. In this study we found of ethoxy- benzyloxy-resorufin-O-dealkylases, p-nitrophenol-hydroxylase erythromycin-N-demethylase (mediated CYP1A, 2B, 2E1 3A, respectively) were depressed, while uridine-glucuronosyl-transferase (a phase 2 enzyme) was unaltered liver microsomes Plasmodium...
The mechanisms by which malaria up and down-regulates CYP activities are not understood yet. It is also unclear whether modulated during non-lethal infections. This study was undertaken to evaluate the time course of alterations in lethal (Plasmodium berghei ANKA) chabaudi chabaudi) murine malaria. Additionally, hypotheses on association depression with enhanced nitric oxide (NO) production, CYP2a5 induction endoplasmic reticulum dysfunction, haem metabolism oxidative stress were examined as...
This study sought to elucidate the reason for traditional use of Psoralea corylifolia (PC) over Heracleum maximum (HM) treating vitiligo, though seeds from both plants are rich in a class phototoxic compounds called furanocoumarins, that effective vitiligo.Toxicity studies were carried out by LD 50 experiments on wistar male and female rats, physiochemical parameters associated with toxicity evaluated.The results correlated elemental profile obtained two using laser-induced breakdown...
Germline inactivating variants in BRCA1 lead to a significantly increased risk of breast and ovarian cancers carriers. While the functional effect many can be inferred from DNA sequence, determining missense present significant challenge. A series biochemical cell biological assays have been successfully used explore impact these on function BRCA1, which contribute assessing their likelihood pathogenicity. It has determined that co-localize with structural or motifs are more likely disrupt...
In this study, we investigated the expression and activity of liver cytochrome P450s (CYPs) praziquantel (PZQ) kinetics in mice infected with Schistosoma mansoni. Swiss Webster (SW) both genders were (100 cercariae) on postnatal day 10 killed post-infection days (PIDs) 30 or 55. Non-infected same age sex served as controls. Regardless mouse sex, infection depressed activities CYP1A [ethoxy/methoxy-resorufin-O-dealkylases (EROD/MROD)], 2B9/10 [pentoxy/benzyloxy-resorufin-O-dealkylases (PROD,...