A5044374036- RNA Research and Splicing
- Prion Diseases and Protein Misfolding
- Trace Elements in Health
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
- Birth, Development, and Health
- Biochemical effects in animals
- Enzyme Structure and Function
- Congenital heart defects research
- Cardiomyopathy and Myosin Studies
- Galectins and Cancer Biology
- Nuclear Structure and Function
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Wnt/β-catenin signaling in development and cancer
- Bacteriophages and microbial interactions
- Protein Hydrolysis and Bioactive Peptides
- Tissue Engineering and Regenerative Medicine
- Alzheimer's disease research and treatments
Universidade Federal do Rio de Janeiro
2019-2025
German Center for Neurodegenerative Diseases
2023
National Education and Research Network
2021-2022
Hospital Universitário Clementino Fraga Filho
2019-2020
National Institute of Science and Technology for Structural Biology and Bioimaging
2019
Structural conversion of cellular prion protein (PrPC) into scrapie PrP (PrPSc) and subsequent aggregation are key events associated with the onset transmissible spongiform encephalopathies (TSEs). Experimental evidence supports role nucleic acids (NAs) in assisting this conversion. Here, we asked whether undergoes liquid-liquid phase separation (LLPS) if process is modulated by NAs. To end, two 25-mer DNA aptamers, A1 A2, were selected against globular domain recombinant murine (rPrP90-231)...
Prion diseases are characterized by prion protein (PrP) transmissible aggregation and neurodegeneration, which has been linked to oxidative stress. The physiological function of PrP seems related sequestering redox-active Cu 2+ , dyshomeostasis is observed in disease brain. It unclear whether contributes aggregation, recently shown be mediated condensation. This study indicates that promotes condensation live cells at the cell surface vitro through copartitioning. Molecularly, inhibited...
Abstract Prion diseases are characterized by prion protein (PrP) transmissible aggregation and toxicity in the brain. The physiological function of PrP seems related to sequestering internalization redox-active Cu 2+ . It is unclear whether contributes aggregation, recently shown be mediated condensation. We investigated role oxidation condensation using multiple biophysical biochemical methods. find that promotes at cell surface vitro through co-partitioning. Molecularly, inhibited...
LMO7 is a multifunctional PDZ-LIM protein that can interact with different molecular partners and found in several intracellular locations. The aim of this work was to shed light on evolution, alternative transcripts, structure gene regulation through multiple silico analyses. We also explored the distribution chicken zebrafish embryonic skeletal muscle cells by means confocal fluorescence microscopy. Our results revealed single mammals, sauropsids, Xenopus holostean fish spotted gar while...
ABSTRACT Structural conversion of cellular prion protein (PrP C ) into scrapie PrP Sc and subsequent aggregation are key events for the onset Transmissible Spongiform Encephalopathies (TSEs). Experimental evidences support role nucleic acids (NAs) in assisting process. Here, we used SELEX methodology to identify two 25-mer DNA aptamers against globular domain recombinant murine (rPrP 90-231 ), namely A1 A2. High-affinity binding A2 rPrP was verified by ITC. Aptamers structure characterized...
ABSTRACT The SARS-CoV-2 nucleocapsid protein (N) is a multifunctional promiscuous nucleic acid-binding protein, which plays major role in assembly and discontinuous RNA transcription, facilitating the template switch of transcriptional regulatory sequences (TRS). Here, we dissect structural features N N-terminal domain (N-NTD), either with or without SR-rich motif (SR), upon binding to single double-stranded TRS DNA, as well their activities for dsTRS melting TRS-induced liquid-liquid phase...
Abstract Zinc is a key component of proteins, including interaction with varying pancreatic hormones, insulin and amylin. in crystallinity ZnT8 knock-out mice models, although the dietary role zinc restriction over both energetic metabolism ß-pancreatic hormonemia morphology remained unexplored. We aimed to test whether on swiss male would impact endocrine pancreas metabolic phenotype. evaluated non-transgenic Swiss weaned onto control or low-zinc diet for 4 weeks. Growth, glycemia,...
Neuropathic pain is a condition with varying origins, including reduced dietary micronutrient intake. Phytate polyphosphate found in seeds and grains that can act as an antinutrient due to the ability of sequester essential divalent metals. Here we tested whether moderate phytate intake could alter nociceptive pain. We subjected weaning mice chow supplemented 1% for eight weeks. Body weight gain, glycemic responses, food ingestion, water liver adipose tissue weights were not altered compared...