A5040396868- Acute Lymphoblastic Leukemia research
- Biochemical and Molecular Research
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- RNA Interference and Gene Delivery
- Protein purification and stability
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Blood Pressure and Hypertension Studies
- Mosquito-borne diseases and control
- Primate Behavior and Ecology
- Endoplasmic Reticulum Stress and Disease
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Animal Behavior and Welfare Studies
- Ubiquitin and proteasome pathways
- Protein Hydrolysis and Bioactive Peptides
- Influenza Virus Research Studies
- Advanced NMR Techniques and Applications
- Pharmacogenetics and Drug Metabolism
Universidade Federal do Rio de Janeiro
2017-2024
National Institute of Science and Technology for Structural Biology and Bioimaging
2017-2024
National Education and Research Network
2021-2022
Universidade Federal do Rio Grande do Norte
2016
Cardiovascular diseases (CVDs), especially arterial hypertension, stand as prominent contributors to global mortality. Regrettably, individuals with diabetes encounter a two-fold increase in the risk of mortality associated CVDs. Hydrochlorothiazide (HCTZ) represents primary intervention for particularly diabetic patients. Nevertheless, there has not yet been comprehensive assessment biophysical characteristics regarding impact glucose levels on its binding affinity human serum albumin...
Immunodiagnostic tests for detecting dengue virus infections encounter challenges related to cross-reactivity with other flaviviruses. Our research focuses on the development of a synthetic multiepitope antigen tailored immunodiagnostics. Selected epitopes involved structural linearity and dissimilarity from proteomes Zika Yellow fever viruses which served computationally modeling three-dimensional protein structure, resulting in design two proteins: rDME-C rDME-BR. Both proteins consist...
<title>Abstract</title> Research on camelid-derived single-domain antibodies (sdAbs) has demonstrated their significant utility in diverse biotechnological applications, including therapy and diagnostic. This is largely due to relative simplicity as monomeric proteins, ranging from 12–15 kDa, contrast immunoglobulin G (IgG) antibodies, which are glycosylated heterotetramers of 150–160 kDa. Single-domain exhibit high conformational stability adopt the typical domain fold, consisting a...
ABSTRACT The SARS-CoV-2 nucleocapsid protein (N) is a multifunctional promiscuous nucleic acid-binding protein, which plays major role in assembly and discontinuous RNA transcription, facilitating the template switch of transcriptional regulatory sequences (TRS). Here, we dissect structural features N N-terminal domain (N-NTD), either with or without SR-rich motif (SR), upon binding to single double-stranded TRS DNA, as well their activities for dsTRS melting TRS-induced liquid-liquid phase...
Abstract: Asparaginase (ASNase) is widely used as an important component of first-line treatment for acute lymphoblastic leukemia (ALL). Although it associated with a high rate complete remission (~93%), challenges remain due to several side effects ranging from immune reactions severe toxicity, largely its higher immunogenicity and glutamine coactivity. Innovative products have therefore been devised minimise these adverse while increasing the enzymes’ pharmacokinetic properties, stability,...
Abstract The hydrolysis of asparagine and glutamine by L-asparaginase has been used to treat acute lymphoblastic leukemia for over four decades. Each monomer a long loop that closes the active site upon substrate binding, acting as lid. Here we present comparative study two commercially available preparations drug containing Escherichia coli L-Asparaginase 2, performed comprehensive array biophysical biochemical approaches. We report oligomeric landscape conformational dynamic plasticity E....
Abstract Amylin is a 37‐residue peptide hormone, which co‐secreted with insulin and prevents postprandial spikes in blood glycemia by slowing gastric emptying promoting satiety. prone to aggregate into oligomers amyloid fibrils, related the onset of type 2 diabetes, hampers its use as biopharmaceutical. To overcome instability extend vivo half‐life it has been proposed conjugation amylin polyethylene glycol (PEG) at HNζ or HNα amines Lys1. Here we used two‐dimensional nuclear magnetic...