A5052061698- Oral microbiology and periodontitis research
- Cellular transport and secretion
- Acute Lymphoblastic Leukemia research
- Metabolomics and Mass Spectrometry Studies
- Biochemical and Molecular Research
- Salivary Gland Disorders and Functions
- SARS-CoV-2 detection and testing
- Radioactive Decay and Measurement Techniques
- SARS-CoV-2 and COVID-19 Research
- Glycosylation and Glycoproteins Research
- Trace Elements in Health
- Biomedical Research and Pathophysiology
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Protein purification and stability
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microtubule and mitosis dynamics
- Nutrition and Health in Aging
- Parasitic Infections and Diagnostics
- Synthesis and Biological Evaluation
- Polyamine Metabolism and Applications
- Advanced Nanomaterials in Catalysis
- Advanced Drug Delivery Systems
- Child Nutrition and Water Access
Instituto Nacional de Metrologia, Qualidade e Tecnologia
2010-2024
National Institute of Science and Technology for Structural Biology and Bioimaging
2010
Brazilian Synchrotron Light Laboratory
2006
The shortage of petroleum reserves and the increase in CO2 emissions have raised global concerns highlighted importance adopting sustainable energy sources. Second-generation ethanol made from lignocellulosic materials is considered to be one most promising fuels for vehicles. giant snail Achatina fulica an agricultural pest whose biotechnological potential has been largely untested. Here, composition microbial population within crop this invasive land snail, as well key genes involved...
Cellulose synthesis in bacteria is a complex process involving the concerted action of several enzymes whose genes are often organized operons. This influences many fundamental physiological aspects such as and host interaction, biofilm formation, among others. Although it might sound contradictory, participation cellulose-degrading critical to this process. The presence endoglucanases from family 8 glycosyl hydrolases (GH8) bacterial cellulose synthase (Bcs) has been described different...
The new complexes Zn(ITZ)2Cl2 (1) and Zn(ITZ)2(OH)2 (2) were synthetized by a reaction of itraconazole with their respective zinc salts under reflux. These Zn-ITZ characterized elemental analyses, molar conductivity, mass spectrometry, 1H 13C{1H} nuclear magnetic resonance, UV-vis infrared spectroscopies. antiparasitic antifungal activity was evaluated against three protozoans medical importance, namely, Leishmania amazonensis, Trypanosoma cruzi, Toxoplasma gondii, two fungi, Sporothrix...
Required metrological tools, such as higher order reference measurements procedures, pure substance and matrix certified materials, are established for small well defined molecules.Difficulties still remain in the provision of SI traceable standards area larger biomolecules peptides/proteins.The Primary Calibration Reference Services has been identified a core technical competency National Measurement Institutes (NMIs).A concept elaborated by Focus Group I on peptide/protein purity strategic...
In this work, we identified human and bacterial proteomes in the saliva from volunteers with gingivitis or healthy.The reported population consisted of 18 (six 12 healthy controls). Proteomics characterization was performed using a quantitative mass spectrometry method.A total 74 116 proteins were saliva. The major functional category that modified proteome immune response, followed by transport protease inhibition. proteome, most Fusobacteria phylum, Chlamydiae Spirochaetes.We observed...
ABSTRACT Purpose Obesity and its associated metabolic disorders, such as T2DM MeS, are a growing public health problem worldwide. Our goal was the identification of protein patterns that uniquely characteristic higher BMI, in Brazilian population. Experimental Design Saliva plasma proteomes, clinical parameters were analyzed population from state Rio de Janeiro, Brazil, mixed‐race Volunteers sorted by their BMI into normal ( n = 29), overweight 25), obese 15) compared with individuals MeS...
The use of nanoparticles (NPs) in the healthcare market is growing exponentially, due to their unique physicochemical properties. Titanium dioxide (TiO2 NPs) are used formulation sunscreens, photoprotective capacity, but interactions these particles with skin cells on nanoscale still unexplored. In present study we aimed determine whether initial nano-biological interactions, namely formation a nano-bio-complex (other than protein corona), can predict rutile internalization and intracellular...
Main text Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P216, was coordinated by Chinese National Institute Metrology (NIM), Research Council Canada (NRC) and Bureau International des Poids et Mesures (BIPM). The global coronavirus pandemic has also led to increased focus on antibody quantitation methods. IgG are among immunoglobulins produced immune system provide protection against SARS-CoV-2....
Periodontal disease is an infectious inflammatory related to the destruction of supporting tissues teeth, leading a functional loss teeth. Inflammatory molecules present in exudate are catalyzed and form different metabolites that can be identified quantified. Thus, we evaluated crevicular fluid identify metabolic biological markers for diagnosing chronic periodontal older adults. Research participants were selected from long-term institutions Brazil. Participants individuals aged 65 years...
Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a key comparison, CCQM-K115.b, was coordinated by Bureau International des Poids et Mesures (BIPM) and Chinese National Institute Metrology (NIM). Seven Institutes or Designated BIPM participated. Participants were required to assign mass fraction oxytocin (OXT) present as main component in comparison sample for CCQM-K115.b. The samples prepared from synthetic OXT purchased...
Main text Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P216, was coordinated by Chinese National Institute Metrology (NIM), Research Council Canada (NRC) and Bureau International des Poids et Mesures (BIPM). Eleven Institutes or Designated BIPM participated in first phase study (Part 1). The purpose this to develop measurement capabilities for larger proteins using recombinant humanized IgG monoclonal...
This study sought to analyze the impact of Metabolic Syndrome (MeS) and Type 2 Diabetes (T2DM) on metabolism their relationship Body Mass Index (BMI) identify potential predictive protein biomarkers for MeS T2DM. The proteomes saliva blood, clinical parameters were analyzed in 103 adult individuals from State Rio de Janeiro, Brazil, a mixed-race population. Healthy sorted by normal (n=29), overweight (n=25) obese (n=15) compared with (n=23) T2DM (n=11). Random forest model revealed that 3...
Main text Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P201, was organised. Five National Metrology Institutes (NMIs) participated in this study and provided total eleven measurement results using six different methods based on three principles. The purpose to develop capabilities for accurate quantification intact haemoglobin (Hb) whereby could be made directly traceable SI. choice protein resulted from...
Obesity is a chronic condition related to multiple comorbidities such as hypertension, type 2 diabetes, periodontal and cardiovascular diseases. can lead metabolic change, creating prolonged low-intensity inflammatory process. This study aims analyze the plasma saliva proteomes of young adults with obesity overweight comparing normal weight individuals, reveal if rise on body mass influences proteomic profiles. The reported population consisted 18 students and/or employees Rio de Janeiro...
Main text Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a key comparison, CCQM-K115.c, was coordinated by Bureau International des Poids et Mesures (BIPM), Health Sciences Authority (HSA) Singapore and Chinese National Institute Metrology (NIM). Nine Institutes or Designated BIPM participated. Participants were required to assign mass fraction glycated hexapeptide HbA1c (GE) present as main component in comparison sample for...
Main text Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a key comparison, CCQM-K115.2018, was coordinated by Bureau International des Poids et Mesures (BIPM), Health Sciences Authority (HSA) Singapore and Chinese National Institute Metrology (NIM). Ten Institutes or Designated BIPM participated. Participants were required to assign mass fraction hexapeptide HbA0 (VE) present as main component in comparison sample for...
Main text The key comparison CCQM-K151 protein quantification: Purity-assessed recombinant contents in buffer solution using Insulin analogue was coordinated by Korea Research Institute of Standards and Science (KRISS) under the auspices Protein Analysis Working Group (PAWG) Consultative Committee for Amount Substance: Metrology Chemistry Biology (CCQM) CCQM. Nine National Institutes Designated participated. Participants were required to assign mass fraction human insulin (Ins) present as...