A5025420327- Metabolomics and Mass Spectrometry Studies
- Trypanosoma species research and implications
- Cardiomyopathy and Myosin Studies
- Congenital heart defects research
- Liver Disease Diagnosis and Treatment
- Cardiovascular Effects of Exercise
- Monoclonal and Polyclonal Antibodies Research
- Chronic Kidney Disease and Diabetes
- Atherosclerosis and Cardiovascular Diseases
- Acute Kidney Injury Research
- Cardiac electrophysiology and arrhythmias
- Insect and Pesticide Research
- Medicinal Plants and Neuroprotection
- Neuroscience of respiration and sleep
- Cholinesterase and Neurodegenerative Diseases
- Obstructive Sleep Apnea Research
- Genetics, Aging, and Longevity in Model Organisms
- Liver physiology and pathology
- Cancer, Hypoxia, and Metabolism
- Plant and animal studies
- Diet and metabolism studies
- Cardiac Arrhythmias and Treatments
- Renal and related cancers
- Peptidase Inhibition and Analysis
- Cellular Mechanics and Interactions
Harvard University
2020-2025
Universidade de São Paulo
2013-2023
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2018-2023
Universidade Federal do Rio Grande do Norte
2023
Instituto do Coração
2022
Pontifícia Universidade Católica do Rio Grande do Sul
2012-2013
Universidade Nove de Julho
2012
Hypertrophic cardiomyopathy (HCM) is caused by pathogenic variants in sarcomere protein genes that evoke hypercontractility, poor relaxation, and increased energy consumption the heart patient risks for arrhythmias failure. Recent studies show missense myosin, molecular motor of sarcomere, are clustered residues participate dynamic conformational states proteins. We hypothesized these conformations essential to adapt contractile output conservation pathophysiology HCM results from...
[Figure: see text].
Abstract Alzheimer’s disease (AD) is the most common form of dementia in elderly, affecting cognitive, intellectual, and motor functions. Different hypotheses explain AD’s mechanism, such as amyloidogenic hypothesis. Moreover, this multifactorial, several studies have shown that gut dysbiosis oxidative stress influence its pathogenesis. Knowing kefir a probiotic used therapies to restore bioactive peptides present it antioxidant properties, we explored biotechnological potential source...
The purpose of the current study was to test hypothesis that dipeptidyl peptidase IV (DPPIV) inhibitor sitagliptin, which exerts anti-hyperglycemic and anti-hypertensive effects, upregulates GLUT4 translocation, protein levels, and/or mRNA expression in heart skeletal muscle spontaneously hypertensive rats (SHRs). Ten days treatment with sitagliptin (40 mg/kg twice daily) decreased plasma DPPIV activity both young (Y, 5-week-old) adult (A, 20-week-old) SHRs similar extents (~85%). However,...
Vascular smooth muscle cells (VSMCs) are thought to assume a quiescent and homogeneous mechanical behavior after arterial tree development phase. However, VSMCs known be molecularly heterogeneous in other aspects their mechanics may play role pathological situations. Our aim was evaluate from different beds terms of proteomics, as well investigate factors that influence this phenotype. obtained seven arteries were studied using optical magnetic twisting cytometry (both static state...
Abstract The use of metabolomic and lipidomic strategies for selecting potential biomarkers obstructive sleep apnoea (OSA) has been little explored. We examined adult male patients with OSA (defined by an apnoea-hypopnoea index ≥15 events/hour), as well age-, gender-, fat-composition-matched volunteers without OSA. All subjects were subjected to clinical evaluation, questionnaires detecting the risk (Berlin NoSAS score), analysis gas chromatography coupled mass spectrometry liquid followed...
Hypertrophic cardiomyopathy is one of the most common inherited cardiomyopathies and a leading cause sudden cardiac death in young adults. Despite profound insights into genetics, there imperfect correlation between mutation clinical prognosis, suggesting complex molecular cascades driving pathogenesis. To investigate this, we performed an integrated quantitative multi-omics (proteomic, phosphoproteomic, metabolomic) analysis to illuminate early direct consequences mutations myosin heavy...
Alzheimer's Disease (AD) is the most common cause of dementia among elderly individuals worldwide, leading to a strong motor-cognitive decline and consequent emotional distress codependence. It traditionally characterized by amyloidogenic pathway formation senile plaques, recent studies indicate that dysbiosis also an important factor in AD's pathology. To overcome dysbiosis, probiotics-as kefir-have shown be great therapeutic alternative for disease. In this present work, we explored kefir...
Chagas cardiomyopathy, a disease caused by Trypanosoma cruzi (T. cruzi) infection, is major contributor to heart failure in Latin America. There are significant gaps our understanding of the mechanism for infection human cardiomyocytes, pathways activated during acute phase disease, and molecular changes that lead progression cardiomyopathy. To investigate effects T. on cardiomyocytes we infected induced pluripotent stem cell-derived (iPSC-CM) with parasite analyzed cellular, molecular,...
Systemic lupus erythematosus (SLE) is a heterogenous autoimmune disease that affects multiple organs, including the heart. The mechanisms of myocardial injury in SLE remain poorly understood. In this study, we engineered human cardiac tissues and cultured them with IgG from patients SLE, without involvement. elevated inflammation exhibited increased binding to apoptotic cells within subjected stress, whereas systolic dysfunction enhanced surface live cardiomyocytes. Functional assays RNA...
Background SMAD2 is a coregulator that binds variety of transcription factors in human development. Heterozygous loss‐of‐function and missense variants are identified patients with congenital heart disease (CHD) or arterial aneurysms. Mechanisms cause distinct cardiovascular phenotypes remain unknown. We aimed to define transcriptional epigenetic effects their role CHD. also assessed the function uncertain significance. Methods Results Rare (minor allele frequency ≤10 −5 ) were exome...
Ecological conditions in the Amazon rainforests are historically favorable for transmission of numerous tropical diseases, especially vector-borne diseases. The high diversity pathogens likely contributes to strong selective pressures human survival and reproduction this region. However, genetic basis adaptation complex ecosystem remains unclear. This study investigates possible footprints rainforest environment by analyzing genomic data 19 native populations. results based on functional...
Atherosclerotic plaque development is closely associated with the hemodynamic forces applied to endothelial cells (ECs). Among these, shear stress (SS) plays a key role in disease since changes flow intensity and direction could stimulate an atheroprone or atheroprotective phenotype. ECs under low oscillatory SS (LSS) show upregulation of inflammatory, adhesion, cellular permeability molecules. On contrary, high laminar (HSS) increase their expression protective anti-inflammatory factors....
Hepatic stellate cells (HSC) play a key role in liver fibrogenesis. Activation of PPARγ and inhibition fibrogenic molecules are potential strategies to block HSC activation differentiation. A number natural products have been suggested antifibrotic effects for the de-activation de-differentiation HSCs. The purpose this study was investigate vitro capsaicin on de-differentiation. results demonstrated that induced quiescent phenotype GRX via activation. Significant decrease COX-2 type I...
Introduction Studies on metabolomics and CKD have primarily addressed incidence defined as a decline eGFR or appearance of albuminuria in the general population, with very few evaluating hard outcomes. In present study, we investigated association between metabolites mortality ESRD cohort. Setting methods Data 454 participants Progredir Cohort Study, Sao Paulo, Brazil were used. Metabolomics was performed by GC-MS (Agilent MassHunter) identified using Agilent Fiehn GC/MS NIST libraries....
Abstract Cardiac fibroblasts are present throughout the myocardium and enriched in microenvironment surrounding ventricular conduction system (VCS). Several forms of arrhythmias linked to VCS abnormalities, but it is still unclear whether malformations cardiomyocyte autonomous or could be crosstalk between different cell types. We reasoned that influence specialization cells. developed 2D 3D culture models neonatal rat cardiac cells assess on cardiomyocytes. Cardiomyocytes adjacent showed a...
One drawback of in vitro cell culturing is the dedifferentiation process that cells experience. Smooth muscle (SMC) also change molecularly and morphologically with long term culture. The main objective this study was to evaluate if culture passages interfere vascular SMC mechanical behavior. were obtained from five different porcine arterial beds. Optical magnetic twisting cytometry (OMTC) used characterize mechanically cultures distinct confocal microscopy/western blotting, cytoskeleton...
Several techniques to induce renal ischemia have been proposed: clamp, PVA particles, and catheter-balloon. We report the development of a controlled, single-insult model unilateral ischemia/reperfusion (I/R) without contralateral nephrectomy, using suitable model, pig. This is balloon-catheter-based percutaneous, interventional radiology procedure. One angioplasty balloon-catheter was placed into right artery inflated for 120 min reperfusion over 24 h. Serial serums were sampled from...