A5007753594- Thyroid Disorders and Treatments
- Metabolism, Diabetes, and Cancer
- Growth Hormone and Insulin-like Growth Factors
- Neuroendocrine regulation and behavior
- Effects and risks of endocrine disrupting chemicals
- Birth, Development, and Health
- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- High Altitude and Hypoxia
- Heart Rate Variability and Autonomic Control
- Cancer, Hypoxia, and Metabolism
- Genetics and Physical Performance
- Biochemical effects in animals
- Hemoglobin structure and function
- Genetics and Neurodevelopmental Disorders
- Urinary Bladder and Prostate Research
- Stress Responses and Cortisol
- Neuroscience of respiration and sleep
- Molecular Biology Techniques and Applications
- Cardiovascular and exercise physiology
- Circadian rhythm and melatonin
- Ion channel regulation and function
- RNA modifications and cancer
- Toxic Organic Pollutants Impact
Universidade Federal de São Paulo
2016-2025
Instituto de Ciências Farmacêuticas
2024
Universidade de São Paulo
2000-2017
University of Chile
2017
Faculdade de Medicina do ABC
2012-2016
Fundação do ABC
2011
Instituto Butantan
2011
Reproductive physiology involves complex biological processes that can be disrupted by exposure to environmental contaminants. The effects of bisphenol A (BPA) on spermatogenesis and sperm quality is still unclear. objective this study was investigate the reproductive toxicity BPA at dosages considered safe (5 or 25mg BPA/kg/day). We assessed multiple parameters, relative expression genes involved in central regulation hypothalamic-pituitary-testicular axis, serum concentrations...
Glyphosate-based herbicides (GBHs) are widely used in agriculture. Recently, several animal and epidemiological studies have been conducted to understand the effects of these chemicals as an endocrine disruptor for gonadal system. The aim present study was determine whether GBHs could also disrupt hypothalamic-pituitary-thyroid (HPT) axis. Female pregnant Wistar rats were exposed a solution containing GBH Roundup®Transorb (Monsanto). animals divided into three groups (control, 5mg/kg/day or...
In the present study, cytotoxicity of palmitic, stearic, oleic, linoleic, arachidonic, docosahexaenoic and eicosapentaenoic acids on a macrophage cell line (J774) was investigated. The induction toxicity investigated by changes in size, granularity, membrane integrity, DNA fragmentation phosphatidylserine externalization using flow cytometry. Fluorescence microscopy used to determine type death (Acridine Orange/ethidium bromide assay). possible mechanisms involved were examined measuring...
The purpose of the current study was to test hypothesis that dipeptidyl peptidase IV (DPPIV) inhibitor sitagliptin, which exerts anti-hyperglycemic and anti-hypertensive effects, upregulates GLUT4 translocation, protein levels, and/or mRNA expression in heart skeletal muscle spontaneously hypertensive rats (SHRs). Ten days treatment with sitagliptin (40 mg/kg twice daily) decreased plasma DPPIV activity both young (Y, 5-week-old) adult (A, 20-week-old) SHRs similar extents (~85%). However,...
The modern concept of thyroid disruptors includes synthetic chemicals and bioactive compounds from food that interfere with any aspect the hypothalamus–pituitary–thyroid axis, hormone biosynthesis secretion, blood transmembrane transport, metabolism local actions hormones. This review highlights relevant affect populations through their diet: directly itself (fish oil polyunsaturated fatty acids, pepper, coffee, cinnamon resveratrol/grapes), vegetable cultivation (pesticides) containers for...
In response to the rapid development of genetically engineered glyphosate-tolerant crops, use glyphosate-based herbicides (GBHs), in agriculture, has increased substantially. Currently, it is estimated that 747 million kg GBHs are applied per year. Although several epidemiological studies have demonstrated there health risks associated with GBH exposure, effects these chemicals on oxidative and inflammatory brain still unclear. fact, alterations processes could contribute neurological...
Glucose transporter 4 (GLUT4) is highly expressed in muscle and fat tissue, where triiodothyronine (T(3)) induces solute carrier family 2 facilitated glucose member (SLC2A4) gene transcription. T(3) was also shown to rapidly increase uptake myocytes exposed cycloheximide, indicating that it might act nongenomically regulate GLUT4 availability. We tested this hypothesis by evaluating, thyroidectomized rats (Tx rats), the acute and/or chronic effects on mRNA expression polyadenylation, protein...
Pyriproxyfen (PPF) is a larvicide used to combat and control insects in agriculture, veterinary public health, with the of vectors such as mosquitoes that transmit dengue fever. Despite...
OBJECTIVE: Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh formalin-fixed/paraffin-embedded tissues is crucial to ensure the success molecular endocrinology techniques, especially when samples are stored for long periods, or no other can be collected patients who lost follow-up. Here, we evaluate several procedures improve current methodologies DNA (salting-out) RNA isolation. MATERIALS AND METHODS: We used proteinase K...
Bisphenol A (BPA) is an endocrine-disrupting chemical (EDC) that widely used in the manufacturing of plastics and inner linings food cans. Previously, it was reported BPA disturbed sexual dimorphic nucleus hypothalamus delaying onset puberty attributed to estrogenic action. In addition, during perinatal period increased LH serum concentrations male offspring dams at doses below reproductive NOAEL (No Observable Adverse Effect Level) based upon World Health Organization guidelines. Based...
Rett syndrome (RTT) is an X-linked neurodevelopmental disorder in which the MECP2 (methyl CpG-binding protein 2) gene mutated. Recent studies showed that RTT-derived neurons have many cellular deficits when compared to control, such as: less synapses, lower dendritic arborization and reduced spine density. Interestingly, treatment of with Insulin-like Growth Factor 1 (IGF1) could rescue some these phenotypes. Given critical role IGF1 during neurodevelopment, present study used human induced...
Thyroid hormone was shown to induce actin cytoskeleton polymerization in hypothyroid astrocytes and osteoblastic cells by a nongenomic mechanism. Polyadenylation of GH mRNA, process that depends on cytoskeleton-associated proteins, also be regulated thyroid hormone. Here we investigated histochemistry immunohistochemistry whether acute (100 μg per 100 g body weight, iv, for 30 min) or chronic (5 ip, 5 d) administration T3 thyroidectomized (Tx) sham-operated rats affects the somatotrophs...
Cytoskeleton controls the stability of transcripts, by mechanisms that involve mRNAs and eEF1A attachment to it. Besides, it plays a key role in protein synthesis secretion, which seems be impaired somatotrophs hypothyroid rats, whose cytoskeleton is disarranged. This study investigated the: GH mRNA binding plus translation rate sham-operated thyroidectomized rats treated with T3 or saline, killed 30min thereafter. Thyroidectomy reduced: (a) pituitary F-actin content, it; (b) recruitment...
Skeletal muscle is a target tissue for approaches that can improve insulin sensitivity in insulin-resistant states. In muscles, glucose uptake performed by the GLUT-4 protein, which encoded SLC2A4 gene. gene expression increases response to conditions sensitivity, including chronic exercise. However, since exercise improves increased could not be clearly attributed contractile activity per se and/or improved sensitivity. The present study was designed investigate role of regulation as well...
Myoglobin (Mb) gene expression, Citrate Synthase (CS) and Succinate Dehydrogenase (SDH) activities of Soleus (S) Extensorum Digitalis Longus (EDL) muscles were studied in intact, thyroidectomized T3-treated (25 microg/100g, BW, ip, 15 days) rats. The fiber type composition S muscle was also evaluated used as control the T3-induced effects. In muscle, T3 treatment increased Mb mRNA protein well CS SDH activity. These changes occurred parallel to expected increase II (fast) decrease I...
T3 regulates transcription of the rat sarcoendoplasmic reticulum calcium ATPase in heart. The effect is mediated by three differently configured response elements (TREs). Here we report mutation each individual TRE promoter and contribution on gene expression. Mutation TRE1, a direct repeat element, exerted strongest response, compared with TRE2 TRE3, which are inverted palindromes. isolated showed no (TRE2) or were weakly positive (TRE3), became strong negative regulatory analog GC-1. We...