A5055963262- 3D Printing in Biomedical Research
- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Radiation Therapy and Dosimetry
- Hematological disorders and diagnostics
- Single-cell and spatial transcriptomics
- Biomedical and Engineering Education
- Spaceflight effects on biology
- Electrospun Nanofibers in Biomedical Applications
- Wound Healing and Treatments
- Angiogenesis and VEGF in Cancer
- Effects of Radiation Exposure
- Diabetic Foot Ulcer Assessment and Management
- Digital Imaging for Blood Diseases
- Adipose Tissue and Metabolism
- Neuroscience and Neural Engineering
- Biomedical Ethics and Regulation
- Immune cells in cancer
- Erythrocyte Function and Pathophysiology
- Systemic Lupus Erythematosus Research
- Hippo pathway signaling and YAP/TAZ
- Problem and Project Based Learning
Columbia University
2020-2025
Columbia University Irving Medical Center
2021-2024
City University of Seattle
2022-2023
New York Stem Cell Foundation
2023
École Polytechnique Fédérale de Lausanne
2019-2022
University of Lausanne
2022
Office of the Chief Medical Examiner
2020
University of Virginia
2015-2019
From micro-scaled capillaries to millimeter-sized arteries and veins, human vasculature spans multiple scales cell types. The convergence of bioengineering, materials science, stem biology has enabled tissue engineers recreate the structure function different hierarchical levels vascular tree. Engineering large-scale vessels been pursued over past thirty years replace or bypass damaged arteries, arterioles, venules, their routine application in clinic may become a reality near future....
Traditional drug screening models are often unable to faithfully recapitulate human physiology in health and disease, motivating the development of microfluidic organs-on-a-chip (OOC) platforms that can mimic many aspects process alleviate discrepancies between preclinical studies clinical trials outcomes. Linsitinib, a novel anti-cancer drug, showed promising results pre-clinical Ewing Sarcoma (ES), where it suppressed tumor growth. However, Phase II trial several European centers with...
Abstract Galactic cosmic radiation (GCR) is one of the most serious risks posed to astronauts during missions Moon and Mars. Experimental models capable recapitulating human physiology are critical understanding effects on organs developing radioprotective measures against space travel exposures. The systemic studied using a multi‐organ‐on‐a‐chip (multi‐OoC) platform containing engineered tissue bone marrow (site hematopoiesis acute damage), cardiac muscle chronic damage) liver metabolism),...
Background: Core binding factor acute myeloid leukemia (AML) is a common type of pediatric AML characterized by inv(16) or t (8;21) lesions that inhibit the function core complex.Although these rearrangements are considered favorable risk in AML, nearly 30% children with will relapse, indicating continued need for improved understanding biology and new therapeutic targets.Mutations cohesin complex genes occur commonly t(8;21) but never found inv( 16) suggesting unique role pathophysiology...
Resident cardiac macrophages are critical mediators of function. Despite their known importance to electrophysiology and tissue maintenance, there currently no stem-cell-derived models human engineered tissues (hECTs) that include resident macrophages. In this study, we made an induced pluripotent stem cell (iPSC)-derived hECT model with a population (iM0) better recapitulate the native myocardium characterized impact on Macrophage retention within hECTs was confirmed via immunofluorescence...
Diabetic retinopathy is characterized by progressive vascular dropout with subsequent vision loss. We have recently shown that an intravitreal injection of adipose-derived stem cells (ASCs) can stabilize the retinal microvasculature, enabling repair and regeneration damaged capillary beds in vivo. Because understanding ASC status from healthy versus diseased donors will be important as autologous cellular therapies are developed for unmet clinical needs, we took advantage hyperglycemic...
Systemic lupus erythematosus (SLE) is a heterogenous autoimmune disease that affects multiple organs, including the heart. The mechanisms of myocardial injury in SLE remain poorly understood. In this study, we engineered human cardiac tissues and cultured them with IgG from patients SLE, without involvement. elevated inflammation exhibited increased binding to apoptotic cells within subjected stress, whereas systolic dysfunction enhanced surface live cardiomyocytes. Functional assays RNA...
Incomplete understanding of metastatic disease mechanisms continues to hinder effective treatment cancer. Despite remarkable advancements toward the identification druggable targets, options for patients in remission following primary tumor resection remain limited. Bioengineered human tissue models sites capable recreating physiologically relevant milieu colonization may strengthen our grasp cancer progression and contribute development therapeutic strategies. We report use an engineered...
Abstract The increasing risks of ionizing radiation (IR) from deep space travel and nuclear accidents necessitate the development effective countermeasures. Acute syndrome (ARS), particularly hematopoietic ARS (H‐ARS), leads to life‐threatening anemia bone marrow failure, with long‐term including cancer cardiovascular disease. This study presents phenylboronic acid‐functionalized chitosan‐polyethylenimine (CPB) polymers designed for efficient oral delivery pegfilgrastim (PF), an FDA‐approved...
The bone marrow (BM) exists heterogeneously as hematopoietic/red or adipocytic/yellow depending on skeletal location, age, and physiological condition. Mouse models patients undergoing radio/chemotherapy suffering acute BM failure endure rapid adipocytic conversion of the microenvironment, so-called "red-to-yellow" transition. Following hematopoietic recovery, such upon transplantation, a "yellow-to-red" transition occurs functional hematopoiesis is restored. Gold Standards to estimate...
Bone marrow adipocytes (BMAds) constitute the most abundant stromal component of adult human bone marrow. Two subtypes BMAds have been described, more labile regulated (rBMAds) and stable constitutive (cBMAds), which develop earlier in life are resilient to environmental metabolic disruptions. In vivo, rBMAds enriched saturated fatty acids, contain smaller lipid droplets (LDs) readily provide hematopoietic support than their cBMAd counterparts. Mouse models used for research, but isolation...
In tissue development and homeostasis, transforming growth factor (TGF)-β signaling is finely coordinated by latent forms matrix sequestration. Optogenetics can offer precise dynamic control of cell signaling. We report the an optogenetic human induced pluripotent stem system for TGF-β demonstrate its utility in directing differentiation into smooth muscle, tenogenic, chondrogenic lineages. Light-activated resulted expression markers at levels close to those soluble factor-treated cultures,...
Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due a lack of sufficient methods examine these parameters in vivo at high resolution throughout an angiogenic network. The availability surrogate markers for functional vascular proteins, such as green fluorescent protein, enables expression individual cells be followed over time using confocal microscopy, while photoacoustic microscopy dynamic measurement blood flow across the network...
Abstract Pulmonary air leak is the most common complication of lung surgery, contributing to post‐operative morbidity in up 60% patients; yet, there no reliable treatment. Available surgical sealants do not match demanding deformation mechanics tissue; and therefore, fail seal leak. To address this therapeutic gap, a sealant with structural mechanical similarity subpleural designed, developed, systematically evaluated. This “lung‐mimetic” hydrofoam material that has alveolar‐like porous...
Oxygen is essential to wound healing; therefore, accurate monitoring can guide clinical decisions. Clinical assessment often subjective, and tools monitor oxygen are typically expensive, indirect, highly variable. This study demonstrates the utility of a novel, low-cost oxygen-sensing thin film for serial oxygenation.Dual-layer films were fabricated with boron nanoparticles (BNPs) impregnated into chitosan-polycaprolactone layer direct bed contact relatively impermeable calcium alginate...
Future lunar missions and beyond will require new innovative approaches to radiation countermeasures. The Translational Research Institute for Space Health (TRISH) is focused on identifying supporting unique reduce risks human health performance future low Earth orbit. This paper describe three funded complementary avenues reducing the risk humans from exposure experienced in deep space. first focus therapeutic targets damaging effects of by focusing high throughput genetic screens...
In the Undergraduate School of Engineering and Applied Sciences (SEAS) at University Virginia (UVa), there are few opportunities for undergraduate students to teach, let alone develop, an introductory course their major. As two engineering (D. N. Tavakol C. J. Broshkevitch), we were among first take advantage a new initiative UVa SEAS offer student-led courses. part this program, designed 1000-level, 1-credit, pass-fail entitled Introduction Research in Regenerative Medicine. During...