A5036838580- Hematopoietic Stem Cell Transplantation
- Traditional and Medicinal Uses of Annonaceae
- Hematological disorders and diagnostics
- Single-cell and spatial transcriptomics
- Microfluidic and Bio-sensing Technologies
- Radiomics and Machine Learning in Medical Imaging
- 3D Printing in Biomedical Research
- Natural product bioactivities and synthesis
- Bone and Joint Diseases
- Insect Pest Control Strategies
- Cancer Cells and Metastasis
- Insect Resistance and Genetics
- Kruppel-like factors research
- Immune cells in cancer
- Phytochemical compounds biological activities
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Diabetic Foot Ulcer Assessment and Management
- Moringa oleifera research and applications
- Medicinal plant effects and applications
- Liver physiology and pathology
- Lymphatic System and Diseases
- Mesenchymal stem cell research
- Cancer, Hypoxia, and Metabolism
- Acute Myeloid Leukemia Research
- Cocoa and Sweet Potato Agronomy
École Polytechnique Fédérale de Lausanne
2019-2024
University of Lausanne
2022-2024
University Hospital of Lausanne
2024
Lebanese American University
2019-2023
Bone marrow fibrosis is the most extensive matrix remodeling of microenvironment and can include de novo formation bone (osteosclerosis). Spatiotemporal information on contribution distinct niche populations to this process incomplete. We demonstrate that fibrosis-inducing hematopoietic cells cause profibrotic reprogramming perivascular CXCL12 abundant reticular (CAR) progenitor resulting in loss their hematopoiesis-support upregulation osteogenic pro-apoptotic programs. In turn,...
Annona cherimola Mill is a large green fruit with black seeds widely known to possess toxic properties due the presence of Annonaceous acetogenins. The present study investigates anti-cancer an ethanolic seed extract on Acute Myeloid Leukemia (AML) cell lines in vitro and elucidates underlying cellular mechanism. anti-proliferative effects various AML normal mesenchymal cells (MSCs) were assessed using WST-1 viability reagent. pro-apoptotic effect was evaluated Annexin V/PI staining Cell...
Abstract Background The edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. current study evaluates the anti-cancer anti-proliferative properties of ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 KG-1). Methods effect A. was evaluated via viability assay. Its pro-apoptotic assessed through Cell Death ELISA dual Annexin V/PI staining. To further investigate molecular mechanism by which promoted...
The bone marrow (BM) exists heterogeneously as hematopoietic/red or adipocytic/yellow depending on skeletal location, age, and physiological condition. Mouse models patients undergoing radio/chemotherapy suffering acute BM failure endure rapid adipocytic conversion of the microenvironment, so-called "red-to-yellow" transition. Following hematopoietic recovery, such upon transplantation, a "yellow-to-red" transition occurs functional hematopoiesis is restored. Gold Standards to estimate...
Malva pseudolavatera Webb & Berthel. is a plant from the Malvaceae family that has long been included in human diet due to its various curative effects. Many leaf extracts species of genus have reported possess anti-cancer properties, however, studies on M. leaves documented anti-inflammatory and anti-oxidant effects with no emphasis their possible potential. The present study explores properties extract acute myeloid leukemia (AML) cell lines vitro deciphers underlying molecular mechanism....
Abstract Background Herbal medicines have been a major target for numerous studies through the past years as an alternative treatment cancer, mainly due to their minimal effects on normal healthy cells. Annona cherimola, popularly known Cherimoya, is edible natural fruit rich in phytochemical components and possess various biological activities. Previous reported anti-cancerous effect of A. cherimola ethanolic leaf extract (AELE) leukemia. This study aims at studying potential anti-cancer...
Urtica dioica (UD) has been widely used in traditional medicine due to its therapeutic benefits, including anticancer effects. Natural compounds have a promising potential when combination with chemotherapeutic drugs. The present study explores the and anti-proliferative properties of UD tea cisplatin on MDA-MB-231 breast cancer cells vitro. To elucidate effect this combination, cell viability assay, Annexin V/PI dual staining, death ELISA, Western blots were performed. results showed that...
Annonaceae family has broad uses in herbal medicine for treatment of several diseases, whether through seeds' or leaves' extracts. The present study investigates the antiproliferative and antitumor activity Annona cherimola aqueous leaf (AAL) extract/infusion acute myeloid leukemia (AML) cell lines vitro. High-resolution LC-MS was first used to analyze composition extract. Cell proliferation assay, Annexin V staining, cycle analysis, dual V/PI death quantification by ELISA, ROS level...
Abstract Adult hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow (BM) niche, which regulates HSPC quiescence, self-renewal, commitment a demand-adapted manner. Although complex BM niche is responsible for adult hematopoiesis, evidence exists simpler, albeit functional more accessible, extramedullary niches. Inspired by anecdotal description of retroperitoneal masses occurring at higher frequency upon hormonal dysregulation within adrenal gland, we hypothesized that...
Abstract Recent advancements in probe-based, full-transcriptome, high-resolution technologies for Formalin-Fixed Paraffin-Embedded (FFPE) tissues, such as Visium CytAssist, Chromium Flex (10X Genomics), and GeoMx DSP (Nanostring), have opened new opportunities studying decades-old archival samples biobanks, facilitating the generation of data from extensive cohorts. However, experimental protocols can be labor-intensive costly; therefore, it is thus essential researchers to carefully...
Abstract Hematopoietic Stem and Progenitor Cells (HSPCs) reside in the hematopoietic niche, a structure that regulates balance of cellular quiescence, self-renewal commitment demand-adapted manner. The bone marrow (BM) niche is formed by several players, mainly endothelial cells, osteoblasts, adipocytes, stromal cells. While BM forms complex structure, evidence exists for simpler, albeit functional, extramedullary niches. However, composition what constitutes simplest unit an HSPC supportive...
Abstract Functional bone marrow studies have focused primarily on hematopoietic progenitors, leaving limited knowledge about other fragile populations, such as adipocytes (BMAds) and megakaryocytes. The isolation of these cells is challenging due to rupture susceptibility large size. We introduce here a label-free cytometry microsystem, MarrowCellDLD, based deterministic lateral displacement. MarrowCellDLD enables the large, BM-derived intrinsic size properties while preserving their...
The first International Summer School on Bone Marrow Adiposity was organized by members of Society and held virtually September 6-8 2021. goal this meeting to bring together young scientists interested in learning about bone marrow adipose tissue biology pathology. Fifty-two researchers from different backgrounds fields, ranging physiopathology hematology, participated the summer school. featured three keynote lectures fundamentals adiposity, scientific workshops technical considerations...
Abstract Functional bone marrow studies have focused primarily on hematopoietic progenitors, leaving limited knowledge about other fragile populations, such as adipocytes (BMAds) and megakaryocytes. The isolation of these cells is challenging due to rupture susceptibility large size. We introduce here a label-free cytometry microsystem, MarrowDLD, based deterministic lateral displacement. MarrowDLD enables the intrinsic size properties while preserving their viability functionality. Bone...
The role of hematopoietic Hedgehog signaling in myeloproliferative neoplasms (MPNs) remains incompletely understood despite data suggesting that (Hh) pathway inhibitors have therapeutic activity patients. We aim to systematically interrogate the canonical vs. non-canonical Hh MPNs. show Gli1 protein levels patient peripheral blood mononuclear cells (PBMCs) mark fibrotic progression and that, murine MPN models, absence Gli1, but not Gli2 or Smo, significantly reduces phenotype fibrosis,...
Abstract Bone marrow (BM) cellularity assessment is a crucial step in the evaluation of BM trephine biopsies for hematological and non-hematological disorders. Clinical based on labor-intensive, semi-quantitative visual estimation hematopoietic adipocytic components by hematopathologists, which does not provide quantitative information other stromal compartments. In this study, we developed validated MarrowQuant 2.0, an efficient user-friendly digital hematopathology workflow integrated...