A5067999659- dental development and anomalies
- Orthodontics and Dentofacial Orthopedics
- Mesenchymal stem cell research
- Oral microbiology and periodontitis research
- Periodontal Regeneration and Treatments
- Immune Response and Inflammation
- Glioma Diagnosis and Treatment
- Bone and Dental Protein Studies
- Temporomandibular Joint Disorders
- Dental Anxiety and Anesthesia Techniques
- Bone Metabolism and Diseases
- Bone health and treatments
- S100 Proteins and Annexins
- Antimicrobial Peptides and Activities
- Advanced Glycation End Products research
- Adenosine and Purinergic Signaling
- Hippo pathway signaling and YAP/TAZ
- Neuroblastoma Research and Treatments
- Molecular Biology Techniques and Applications
- Connective tissue disorders research
- Testicular diseases and treatments
- NF-κB Signaling Pathways
- Cancer Mechanisms and Therapy
- Hops Chemistry and Applications
- Biotin and Related Studies
Universitätsklinikum Aachen
2020-2025
RWTH Aachen University
2020-2025
University of Bonn
2013-2018
University Hospital Bonn
2013-2017
Universidade Federal de São Paulo
2006-2016
Max Delbrück Center
2013
Abstract Objective Resveratrol is a plant polyphenol known for its anti-inflammatory and pro-regenerative properties. These could be beneficial in controlling potential side effects of orthodontic treatment, such as apical root resorption. Orthodontic tooth movement occurs part sterile inflammatory response. However, dysregulation this process can result pathologically increased osteoclast activity the radicular vicinity, leading to unwanted Previous studies have shown that cementum cells...
Bone marrow fibrosis is the most extensive matrix remodeling of microenvironment and can include de novo formation bone (osteosclerosis). Spatiotemporal information on contribution distinct niche populations to this process incomplete. We demonstrate that fibrosis-inducing hematopoietic cells cause profibrotic reprogramming perivascular CXCL12 abundant reticular (CAR) progenitor resulting in loss their hematopoiesis-support upregulation osteogenic pro-apoptotic programs. In turn,...
Abstract Hypercementosis has been previously reported in mice lacking progressive ankylosis protein (Ank knockout mice, or Ank KO) due to decreased levels of the mineralization inhibitor, inorganic pyrophosphate. However, impact hypercementosis on alveolar bone remodeling and periodontal ligament maintenance from orthodontic forces during tooth movement (OTM) remains unclear. To investigate roles ANK movement, (PDL) maintenance, remodeling, root resorption, we performed a split-mouth model...
Mechanical compression simulating orthodontic tooth movement in vitro models induces pro-inflammatory cytokine expression periodontal ligament (PDL) cells. Our previous work shows that TLR4 is involved this process. Here, primary PDL cells are isolated and characterized to better understand the cell signaling downstream of key molecules process sterile inflammation via TLR4. The monoclonal blocking antibody significantly reverses upregulation phospho-AKT, caused by compressive force, levels...
The structural process of bone and periodontal ligament (PDL) remodeling during long-term orthodontic tooth movement (OTM) has not been satisfactorily described yet. Although the mechanism changes in directly affected alveolar deeply investigated, detailed knowledge about specific PDL its interaction with OTM is missing. This work aims to provide an accurate user-independent analysis following a prolonged treatment mice. Orthodontic forces were applied using Ni-Ti coil-spring split-mouth...
// Michael Ehrhardt 1, * , Rogerio B. Craveiro Martin I. Holst 2 Torsten Pietsch Dagmar Dilloo 1 Department of Pediatric Hematology and Oncology, Center for Pediatrics, University Bonn Medical Center, D-53113 Bonn, Germany Neuropathology, 53105 These authors have contributed equally to this work Correspondence to: Rogério Craveiro, e-mail: Rogerio.craveiro@ukb.uni-bonn.de Keywords: medulloblastoma, GDC-0941, PI3K inhibitor, targeted therapy, c-myc Received: August 13, 2014 Accepted: November...
It was the aim of present investigation to examine whether stimulating effect parathyroid hormone (PTH) on human periodontal ligament (hPDL) cell proliferation and differentiation would be enhanced by hPDL/T-cell interaction involving Wnt10b signaling as a mediating pathway. hPDL cells were cultured from healthy premolar tissues three adolescent orthodontic patients exposed PTH(1-34) in monocultures or co-cultures with CD8 + T cells. At harvest, proliferation, alkaline phosphatase-specific...
Human Periodontal Ligament Fibroblasts (hPDLF), as part of the periodontal apparatus, modulate inflammation, regeneration and bone remodeling. Interferences are clinically manifested attachment loss, tooth loosening root resorption. During orthodontic movement (OTM), remodeling adaptation periodontium is required in order to enable movement. hPDLF involvement early phase-OTM compression side was investigated for a 72-h period through well-studied vitro model. Changes morphology, cell...
Abstract Xanthohumol (XN) is a prenylated plant polyphenol that naturally occurs in hops and its products, e.g. beer. It has shown to have anti-inflammatory angiogenesis inhibiting effects it prevents the proliferation of cancer cells. These could be particular interesting for processes within periodontal ligament, as previous studies orthodontic tooth movement associated with sterile inflammatory reaction. Based on this, study evaluates effect XN cementoblasts an vitro model early phase by...
Medulloblastoma (MB) is one of the most common pediatric cancers, likely originating from abnormal development cerebellar progenitor neurons. MicroRNA (miRNA) has been shown to play an important role in central nervous system. Microarray analysis was used investigate miRNA expression desmoplastic MB patients diagnosed at a young age (1 or 2 years old). Normal fetal newborn cerebellum as control. A total 84 differentially expressed miRNAs (64 downregulated and 20 upregulated) were found. Most...
Periodontal defects’ localization affects wound healing and bone remodeling, with faster in the upper jaw compared to lower jaw. While differences blood supply, innervation, odontogenesis contribute, cell-intrinsic variances may exist. Few studies explored cell signaling periodontal ligament stem cells (PDLSC), overlooking mandible-maxilla disparitiesUsing kinomics technology, we investigated molecular PDLSC. Characterization involved surface markers, proliferation, differentiation...
Abstract Bone resorption is driven through osteoclast differentiation by macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear kappa-Β ligand (RANKL). We noted that a disintegrin metalloproteinase (ADAM) 10 ADAM17 are downregulated at the expression level during murine monocytic cell line RAW264.7 in response to RANKL. Both proteinases well known shed variety single-pass transmembrane molecules from surface. further showed inhibitors ADAM10 or promote osteoclastic...
Abstract Prognosis in patients suffering from high‐risk, refractory and relapsed germ cell tumours ( GCT ) often comprising of CD 30‐positive embryonal carcinoma EC components remains poor. Thus, novel treatment strategies are warranted. The antibody‐drug conjugate ADC brentuximab vedotin delivers the potent antimitotic drug monomethyl auristatin E MMAE to 30‐expressing tumour cells. After 30 binding, internalization intracellular linker cleavage cytotoxic can efflux eradicate neighbouring...
Clinical experience indicates that wounds in alveolar bone and periodontal tissue heal faster more efficiently the maxilla compared with mandible. Since stem cells are known to have a decisive influence on wound healing regeneration, aim of this study was determine whether differences proliferation differentiation ligament (PDLSC) from upper (u-PDLSC) lower jaw (l-PDLSC) contribute enhanced maxilla.u-PDLSC l-PDLSC same donor were harvested extracted human maxillary mandibular third molars....
Abstract Chicken acidic leucine‐rich EGF‐like domain‐containing brain protein (CALEB), also known as chondroitin sulfate proteoglycan (CSPG)5 or neuroglycan C, is a neural sulfate‐containing and epidermal growth factor (EGF)‐domain‐containing transmembrane that implicated in synaptic maturation. Here, we studied the role of CALEB within developing cerebellum. Adult ‐deficient mice displayed impaired motor coordination Rota‐Rod experiments. Analysis neuronal connectivity Purkinje cells by...
High mobility group box protein-1 (HMGB1) is mainly recognized as a chemoattractant for macrophages in the initial phase of host response to pathogenic stimuli. However, recent findings provide evidence anabolic properties terms enhanced proliferation, migration, and support wound healing capacity mesenchymal cells suggesting dual role cytokine regulation immune subsequent regenerative processes. Here, we examined potential effects HMGB1 on human periodontal ligament (PDL) remodelling,...
// Rogerio B. Craveiro 1,* , Michael Ehrhardt Martin I. Holst 2 Thorsten Pietsch and Dagmar Dilloo 1 Department of Pediatric Hematology Oncology, Center for Pediatrics, University Bonn Medical Center, Bonn, Germany Neuropathology, * These authors contributed equally to this work Correspondence: Rogério Craveiro, email: Keywords : Medulloblastoma, Sorafenib, Pazopanib, Targeted therapy, Multi-kinase inhibitor (MKI) Received May 05, 2014 Accepted July 21, Published 22, Abstract Regardless the...
Abstract Different structures and cell types of the periodontium respond to orthodontic tooth movement (OTM) individually. Cementoblasts (OC/CM) located in immediate vicinity fibroblasts on cement have found way centre actual research. Here, we identify validate possible reference genes for OC/CM cells by RT-qPCR with without static compressive loading. We investigated suitability 3 an vitro model cementoblast using four different algorithms (Normfinder, geNorm, comparative delta-C t method...
Abstract Objectives To evaluate post-treatment movements of lower anterior teeth during orthodontic retention in patients with fixed twistflex retainers versus those combined and removable retainers. Materials Methods This study was based on a retrospective data analysis 57 adult retention. They were assigned to two groups: In group 1 ( n = 30) the jaw provided only 2 27) retainer for night-time use. Orthodontic models digitalized superimposed. Tooth analyzed at bonding (t0) follow-up...