A5044612266- Polyomavirus and related diseases
- Plant Virus Research Studies
- Hematopoietic Stem Cell Transplantation
- Phagocytosis and Immune Regulation
- Full-Duplex Wireless Communications
- Inhalation and Respiratory Drug Delivery
- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Pregnancy and preeclampsia studies
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Prenatal Screening and Diagnostics
- Organ Transplantation Techniques and Outcomes
- CRISPR and Genetic Engineering
- Neonatal Respiratory Health Research
- 3D Printing in Biomedical Research
- Computational Drug Discovery Methods
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Liver physiology and pathology
- IL-33, ST2, and ILC Pathways
- Cancer Cells and Metastasis
- Pituitary Gland Disorders and Treatments
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
University Hospital of Zurich
2022-2024
University of Zurich
2023
Indian Institute of Science Bangalore
2021-2022
National Centre for Biological Sciences
2019-2021
Imperial College London
2019-2021
Tata Institute of Fundamental Research
2019-2020
Group 1 innate lymphoid cells (ILCs) comprising circulating natural killer (cNK) and tissue-resident ILC1s are critical for host defense against pathogens tumors. Despite a growing understanding of their role in homeostasis disease, the ontogeny group ILCs remains largely unknown. Here, we used fate mapping single-cell transcriptomics to comprehensively investigate origin turnover murine ILCs. Whereas cNK continuously replaced throughout life, uncovered tissue-dependent development ILC1s. A...
Notch signaling is essential for the emergence of definitive hematopoietic stem cells (HSCs) in embryo and their development fetal liver niche. However, how activated which cell type provides ligand receptor activation HSCs unknown. Here we provide evidence that endothelial Jagged1 (Jag1) has a critical early role vascular but not required function during HSC expansion. We demonstrate Jag1 expressed many liver, including HSCs, its expression lost adult bone marrow HSCs. Deletion does affect...
Abstract Adult hematopoietic stem and progenitor cells (HSPCs) reside in the bone marrow (BM) niche, which regulates HSPC quiescence, self-renewal, commitment a demand-adapted manner. Although complex BM niche is responsible for adult hematopoiesis, evidence exists simpler, albeit functional more accessible, extramedullary niches. Inspired by anecdotal description of retroperitoneal masses occurring at higher frequency upon hormonal dysregulation within adrenal gland, we hypothesized that...
Metastatic progression is a major cause of mortality in cervical cancers, but factors regulating migratory and pre-metastatic cell populations remain poorly understood. Here, we sought to assess whether SUV39H1-low chromatin state promotes using meta-analysis data from The Cancer Genome Atlas (TCGA), immunohistochemistry, genomics functional assays. Cervical cancer cells sorted based on ability vitro have low levels SUV39H1 protein, knockdown enhanced migration. Further, TCGA tumours...
Merkel cell carcinoma (MCC) is a rare, aggressive skin cancer caused either by polyomavirus (MCV) T antigen expression, post integration (~80% cases), or UV-mediated DNA damage. Interestingly, overall survival of MCV-positive patients better, making this differential information significant diagnostic and prognostic value. Also, MCV provides direct target for therapy in MCC patients. Currently, the methods used diagnosis tumours are often discordant unreliable. Here we guided molecular...
Abstract Hematopoietic Stem and Progenitor Cells (HSPCs) reside in the hematopoietic niche, a structure that regulates balance of cellular quiescence, self-renewal commitment demand-adapted manner. The bone marrow (BM) niche is formed by several players, mainly endothelial cells, osteoblasts, adipocytes, stromal cells. While BM forms complex structure, evidence exists for simpler, albeit functional, extramedullary niches. However, composition what constitutes simplest unit an HSPC supportive...
Summary The fetal liver (FL) plays a fundamental role in the ontogeny of hematopoietic system, by transiently providing fertile microenvironment for maturation, proliferation and expansion progenitors, as well definitive stem cells (HSCs). Nonetheless, cellular make up identity HSC niches FL remain poorly described. Here we employed combination 3D quantitative microscopy, bulk mRNA-seq, flow cytometry cell-specific reporter models, to dissect spatiotemporal dynamics putative niche HSCs...
Abstract Merkel cell carcinoma (MCC) is a rare, aggressive skin cancer caused either by polyomavirus (MCV) T antigen expression, post integration (∼80% cases), or UV mediated DNA damage. Interestingly, overall survival of patients suffering from MCV positive better, making this differential information significant diagnostic and prognostic value. Also, as causative agent also provides direct target for therapy in virus MCC patients. Currently, the methods used diagnosis tumours are often...
ABSTRACT Merkel cell carcinoma (MCC) is a rare, aggressive skin cancer caused either by polyomavirus (MCPyV) T antigen gene expression, post integration (∼80% cases), or UV mediated DNA damage. Viral-positive tumors are not only but also oncogenically addicted to tumor expression. In this study we used CRISPR-Cas9 based gene-editing develop potential therapeutic tool for MCPyV positive MCC. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas system genome editing...
Abstract Humans are exposed to numerous synthetic foreign particulates in the form of environmental pollutants and diagnostic or therapeutic agents. Specialized immune cells (phagocytes) clear these by phagocytosing attempting degrade them. The process recognition internalization may trigger changes function phagocytes. Some changes, especially ability a particle-loaded phagocyte take up neutralize pathogens, remains poorly studied. Herein, we demonstrate that uptake non-stimulatory...