A5058711049- Immune Response and Inflammation
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Spectroscopy Techniques in Biomedical and Chemical Research
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Nitric Oxide and Endothelin Effects
- Glycosylation and Glycoproteins Research
- Glioma Diagnosis and Treatment
- Photodynamic Therapy Research Studies
- Bacterial Genetics and Biotechnology
- Vitamin C and Antioxidants Research
- Sepsis Diagnosis and Treatment
- Escherichia coli research studies
- Surgical Simulation and Training
- Inflammation biomarkers and pathways
- Porphyrin and Phthalocyanine Chemistry
- Vibrio bacteria research studies
- Anatomy and Medical Technology
- Luminescence and Fluorescent Materials
- Bacteriophages and microbial interactions
- Nanoplatforms for cancer theranostics
Indian Institute of Science Bangalore
2016-2025
Indian Academy of Sciences
2025
Chemnitz University of Technology
2023
Indra (Spain)
2017-2023
Imperial College Healthcare NHS Trust
2013-2019
Charing Cross Hospital
2011-2019
European Synchrotron Radiation Facility
2018
University of Warwick
2018
Division of Undergraduate Education
2018
Bangalore University
2017
LMP2, LMP7, and MECL are interferon γ–inducible catalytic subunits of vertebrate 20S proteasomes, which can replace constitutive (delta, X, Z, respectively) during proteasome biogenesis. We demonstrate that requires LMP2 for efficient incorporation into preproteasomes, preproteasomes containing require LMP7 maturation. The latter effect depends on the presequence but not activity. This cooperative mechanism favors assembly homogeneous “immunoproteasomes” all three inducible subunits,...
Abstract We show that six proteasome-associated proteins are induced by IFN-gamma, corresponding to three proteasome beta-type subunits and their precursors: the MHC-linked (LMP-2 LMP-7) LMP-10. Concurrently, incorporation of LMP-9, LMP-17, LMP-19 into proteasomes is reduced. LMP-10 appears be product a previously cloned subunit gene, MECL-1. MECL-1 transcription increased in presence whereas two other genes, Lmp-15 Lmp-3, not affected. The IFN-gamma-inducible constitutively expressed...
In this work, we report a large-area fabrication of flexible superhydrophobic bactericidal surface decorated with copper hydroxide nanowires. This involves simple two-step method which growth followed by transfer the nanowires onto polydimethylsiloxane (PDMS) mechanical peeling. Additional roughness in PDMS is obtained through incomplete wetting nanoscale gaps leads to dual-scale and superhydrophobicity contact angle 169° hysteresis less than 2°. The simplicity process makes it low-cost...
CD28, a cell surface molecule expressed on all murine T cells, plays critical role in activation. We show here that NK-1.1+ subpopulation of SCID splenocytes, and IL-2-activated NK cells express although at lower levels than alpha beta cells. Optimal proliferation highly purified asialo GM1+ from the spleen was observed response to stimulation with IL-2 PMA, together anti-CD28 or L-B7+ Thus, addition IL-2, require CD28-mediated costimulatory signals for optimal proliferation. produced...
Insects and plants exhibit bactericidal behavior through nanostructures, which leads to physical contact killing that does not require antibiotics or chemicals. Also, certain metallic ions (e.g., Ag+ Cu2+) are well-known kill bacteria by disrupting their cellular functionalities. The aim of this study is explore the improvement in activity combining extreme structure with surface chemistry. We have fabricated tall (8-9 μm high) nanostructures on silicon surfaces (NSS) having sharp tips...
T cells play a critical role in the adaptive immune response of body, especially against intracellular pathogens and cancer. In vitro, cell activation studies typically employ planar (two-dimensional, 2D) culture systems that do not mimic native cell-to-extracellular matrix (ECM) interactions, which influence activation. The goal this work was to study responses line (EL4) primary mouse three-dimensional (3D) bioprinted matrices varied stiffness. Cell-laden hydrogels were 3D from gelatin...
T cells expressing alpha beta- and gamma delta-TCR are associated with the murine vaginal epithelium. A combination of phenotypic analysis cell surface Ag molecular gamma- delta-genes was used to demonstrate that delta-T have several features distinguish them from present in other tissues. Three color flow cytofluorometric demonstrated freshly isolated CD4-CD8- their expression Thy-1 is strain dependent. Furthermore, specific differences CD5, CD28, p55IL-2 receptor were found between tissue....
Mycobacterium indicus pranii (MIP) is approved for use as an adjuvant (Immuvac/Cadi-05) in the treatment of leprosy. In addition, its efficacy being investigated clinical trials on patients with tuberculosis and different tumors. To evaluate delineate mechanisms by which autoclaved MIP enhances anti-tumor responses, growth solid tumors consisting Sp2/0 (myeloma) EL4 (thymoma) cells was studied BALB/c C57BL/6 mice, respectively. Treatment mice a single intra-dermal (i.d.) injection 3 days...
Abstract Background Recent studies have suggested that the use of robotic surgery for prostatectomy has been increasing, but characterization diffusion in other procedures not available. Methods Data were analysed years 2006–2014 using hospital episode statistics (HES), a database all admissions to National Health Service (NHS) hospitals England. OPCS codes used determine annual number prostatectomy, partial nephrectomy, and total abdominal hysterectomy procedures. Concurrent then identify...
Introduction. The literature reflects a resurgence of interest in endoscopic and keyhole endoscope-assisted neurosurgical approaches as alternatives to conventional microsurgical carefully selected cases. aim this study was assess the technical challenges neuroendoscopy, scope for technological innovations overcome these barriers. Materials methods. All full members Society British Neurosurgeons (SBNS) were electronically invited participate an online survey. open-ended structured survey...
Over the last decade, image guidance systems have been widely adopted in neurosurgery. Nonetheless, evidence supporting use of these surgery remains limited. The aim this study was to compare simultaneously effectiveness and safety various against that standard surgery.In preclinical, randomized study, 50 novice surgeons were allocated one following groups: 1) no guidance, 2) triplanar display, 3) always-on solid overlay, 4) wire mesh 5) on-demand inverse realism overlay. Each participant...
The ability of bacteria to form biofilms increases their survival under adverse environmental conditions. Biofilms have enormous medical and impact; consequently, the factors that influence biofilm formation are an important area study. In this investigation, roles two cold shock proteins (CSP) during were investigated in Salmonella Typhimurium, which is a major foodborne pathogen. Among all CSP transcripts studied, expression cspE (STM14_0732) was higher growth. deletion strain (ΔcspE) did...
There has been a steep rise in the emergence of antibiotic-resistant bacteria past few years. A timely diagnosis can help initiating appropriate antibiotic therapy. However, conventional techniques for diagnosing resistance are time-consuming and labor-intensive. Therefore, we investigated potential Raman spectroscopy as rapid surveillance technology tracking resistance. In this study, used to differentiate clinical isolates -sensitive Escherichia coli, Acinetobacter baumannii, Enterobacter...
PepN and its homologues are involved in the ATP-independent steps (downstream processing) during cytosolic protein degradation. To obtain insights into contribution of to peptidase activity Escherichia coli , hydrolysis a selection endopeptidase exopeptidase substrates was studied extracts wild-type strains two pepN mutants, 9218 DH5 α Δ . Hydrolysis three seven tested reduced both mutants. Similar studies revealed that 10 14 greatly This decreased ability cleave these is -specific as there...