A5084721632- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Cancer therapeutics and mechanisms
- Effects of Radiation Exposure
- Spaceflight effects on biology
- Advanced biosensing and bioanalysis techniques
- Quantum Dots Synthesis And Properties
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- bioluminescence and chemiluminescence research
- Viral gastroenteritis research and epidemiology
- Antimicrobial Peptides and Activities
- Advanced Nanomaterials in Catalysis
- SARS-CoV-2 detection and testing
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Nanoplatforms for cancer theranostics
- Salmonella and Campylobacter epidemiology
- HIV Research and Treatment
- Antimicrobial agents and applications
- Optimism, Hope, and Well-being
Sandia National Laboratories California
2019-2024
University of Colorado Boulder
2013-2023
BioWorks (United States)
2022
District of Columbia Department of Health
2021
University of Puerto Rico System
2013
The rise of multidrug-resistant (MDR) bacteria is a growing concern to global health and exacerbated by the lack new antibiotics. To treat already pervasive MDR infections, classes antibiotics or antibiotic adjuvants are needed. Reactive oxygen species (ROS) have been shown play role during antibacterial action; however, it not yet understood whether ROS contribute directly an outcome bacterial lethality caused We show that light-activated nanoparticle, designed produce tunable flux specific...
As the world braces to enter its fourth year of coronavirus disease 2019 (COVID-19) pandemic, need for accessible and effective antiviral therapeutics continues be felt globally. The recent surge Omicron variant cases has demonstrated that vaccination prevention alone cannot quell spread highly transmissible variants. A safe nontoxic therapeutic with an adaptable design respond emergence new variants is critical transitioning treatment COVID-19 as endemic disease. Here, we present a novel...
The devastating effects of the coronavirus disease 2019 (COVID-19) pandemic have made clear a global necessity for antiviral strategies. Most fatalities associated with infection from severe acute respiratory syndrome 2 (SARS-CoV-2) result at least partially uncontrolled host immune response. Here, we use an antisense compound targeting previously identified microRNA (miRNA) linked to cases COVID-19. binds specifically miRNA in question, miR-2392, which is produced by human cells several...
The development of specific, safe, and potent monoclonal antibodies (Abs) has led to novel therapeutic options for infectious disease. In addition preventing viral infection through neutralization, Abs can clear infected cells induce immunomodulatory functions engagement their crystallizable fragment (Fc) with complement proteins Fc receptors on immune cells. Little is known about the role effector neutralizing in context encephalitic alphavirus infection. To determine function efficacy...
Reactive oxygen species (ROS) represent a broad range of chemical including superoxide, hydroxyl, singlet oxygen, and hydrogen peroxide. Each behaves differently in the cellular environment. Some can play specific roles as intracellular signaling molecules, while others act primarily indiscriminate oxidants. Several recent reports have promoted use exogenous ROS therapeutic agents with applications from cancer therapies to novel antimicrobials. However, therapeutics, specifically...
Abstract Multidrug-resistant (MDR) bacteria pose a grave concern to global health, which is perpetuated by lack of new treatments and countermeasure platforms combat outbreaks or antibiotic resistance. To address this, we have developed Facile Accelerated Specific Therapeutic (FAST) platform that can develop effective peptide nucleic acid (PNA) therapies against MDR within week. Our FAST uses bioinformatics toolbox design sequence-specific PNAs targeting non-traditional pathways/genes...
The rapid emergence of superbugs or multi-drug resistant (MDR) organisms has prompted a search for novel antibiotics, beyond traditional small-molecule therapies. Nanotherapeutics are being investigated as alternatives, and recently superoxide-generating quantum dots (QDs) have been shown important candidates selective light-activated therapy potentiating existing antibiotics against MDR superbugs. Their therapeutic action is selective, can be tailored by simply changing their...
The respiratory virus responsible for coronavirus disease 2019 (COVID-19), severe acute syndrome 2 (SARS-CoV-2), has affected nearly every aspect of life worldwide, claiming the lives over 3.9 million people globally, at time this publication. Neutralizing humanized nanobody (VHH)-based antibodies (VHH-huFc) represent a promising therapeutic intervention strategy to address current SARS-CoV-2 pandemic and provide powerful toolkit future outbreaks. Using synthetic, high-diversity VHH...
The recent surge of drug-resistant superbugs and shrinking antibiotic pipeline are serious challenges to global health. In particular, the emergence β-lactamases has caused extensive resistance against most frequently prescribed class β-lactam antibiotics. Here, we develop novel synthetic peptide nucleic acid-based antisense inhibitors that target start codon ribosomal binding site TEM-1 β-lactamase transcript act via translation inhibition mechanism. We show these capable resensitizing...
Future lunar missions and beyond will require new innovative approaches to radiation countermeasures. The Translational Research Institute for Space Health (TRISH) is focused on identifying supporting unique reduce risks human health performance future low Earth orbit. This paper describe three funded complementary avenues reducing the risk humans from exposure experienced in deep space. first focus therapeutic targets damaging effects of by focusing high throughput genetic screens...
The economical production of chemicals and fuels by microbial processes remains an intense area interest in biotechnology. A key limitation such efforts concerns the availability co-factors, this case NADPH, required for target pathways. Many strategies pursued increasing NADPH Escherichia coli involve manipulations to central metabolism, which can create redox imbalances overall growth defects. In study we used a reactive oxygen species based selection search novel methods availability. We...
Nanomaterials have been extensively used in the biomedical field and recently garnered attention as potential antimicrobial agents. Cadmium telluride quantum dots (QDs) with a bandgap of 2.4 eV (CdTe-2.4) were previously shown to inhibit multidrug-resistant clinical isolates bacterial pathogens via light-activated superoxide generation. Here we investigate transcriptomic response Escherichia coli phototherapeutic CdTe-2.4 QDs both without illumination, well comparison...
Antisense transcription is omnipresent occurring broadly in most living organisms.Growing evidence suggests the presence of noncoding cis-antisense RNA's that can silence gene expression.Recent studies also indicate role transcriptional interference regulating expression neighboring genes arranged convergent orientation.A combination and RNA interaction has potential to add multiple-levels regulation which allow such a system have tunable complex higher-order response environmental...
Antibacterial resistance necessitates the development of novel treatment methods for infections. Protein aggregates have recently been applied as antimicrobials to disrupt bacterial homeostasis. Past work on protein has focused genome mining aggregation-prone sequences in genomes rather than rational design aggregating antimicrobial peptides. Here, we use a synthetic biology approach an artificial gene encoding de novo peptide. This gene, opaL (overexpressed aggregator lipophilic), disrupts...
ABSTRACT Multidrug-resistant (MDR) bacteria pose a grave concern to global health. This problem is further aggravated by lack of new and effective antibiotics countermeasure platforms that can sustain the creation novel antimicrobials in wake outbreaks or evolution resistance antibiotics. To address this, we have developed Facile Accelerated Specific Therapeutic (FAST) platform develop therapies against MDR within week. Our FAST combines four essential modules- design, build, test,...