A5068157493- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Gut microbiota and health
- Viral Infections and Outbreaks Research
- SARS-CoV-2 and COVID-19 Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Complement system in diseases
- HIV Research and Treatment
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Fungal Infections and Studies
- Extracellular vesicles in disease
- Inflammasome and immune disorders
- Probiotics and Fermented Foods
- Bacterial Identification and Susceptibility Testing
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Cancer-related molecular mechanisms research
Lawrence Livermore National Laboratory
2021-2025
Abstract Engineered monoclonal antibodies have proven to be highly effective therapeutics in recent viral outbreaks. However, despite technical advancements, an ability rapidly adapt or increase antibody affinity and by extension, therapeutic efficacy, has yet fully realized. We endeavored stand‐up such a pipeline using molecular modeling combined with experimental library screening the of F5, potent neutralizing activity against Venezuelan Equine Encephalitis Virus (VEEV), recombinant VEEV...
The development of specific, safe, and potent monoclonal antibodies (Abs) has led to novel therapeutic options for infectious disease. In addition preventing viral infection through neutralization, Abs can clear infected cells induce immunomodulatory functions engagement their crystallizable fragment (Fc) with complement proteins Fc receptors on immune cells. Little is known about the role effector neutralizing in context encephalitic alphavirus infection. To determine function efficacy...
Rift Valley fever virus (RVFV) is a highly pathogenic mosquito-borne capable of causing hepatitis, encephalitis, blindness, hemorrhagic syndrome, and death in humans livestock. Upon aerosol infection with RVFV, the brain major site viral replication tissue damage, yet pathogenesis this organ has been understudied. Here, we investigated immune response RVFV infected mice. In to infection, microglia initiated robust transcriptional upregulation antiviral genes, as well increased levels...
The respiratory virus responsible for coronavirus disease 2019 (COVID-19), severe acute syndrome 2 (SARS-CoV-2), has affected nearly every aspect of life worldwide, claiming the lives over 3.9 million people globally, at time this publication. Neutralizing humanized nanobody (VHH)-based antibodies (VHH-huFc) represent a promising therapeutic intervention strategy to address current SARS-CoV-2 pandemic and provide powerful toolkit future outbreaks. Using synthetic, high-diversity VHH...
ABSTRACT Coccidioidomycosis, or Valley fever, is a lung disease caused by inhalation of Coccidioides fungi, prevalent in the Southwestern United States, Mexico, and parts Central South America. Annually, States reports 10,000–20,000 cases, although those numbers are expected to increase as climate change expands fungal geographic range. While 60% infections asymptomatic, 40% symptomatic often misdiagnosed due similarities with bronchitis pneumonia. A small subset infection progress severe...
Neurotropic alphaviruses such as Venezuelan equine encephalitis virus (VEEV) are critical human pathogens that continually expand to naïve populations and for which there no licensed vaccines or therapeutics. VEEV is highly infectious via the aerosol route a recognized weaponizable biothreat causes neurological disease in humans. The neuropathology of has been attributed an inflammatory immune response brain yet underlying mechanisms specific cell involved not fully elucidated. This study...
For years, the airway microbiota have been theorized to be gatekeepers of respiratory health, as pathogens entering make contact with resident microbes prior or coincident their interaction host cells. Thus, modification native community may serve a means altering local environment in favor health. While probiotic supplementation prevent pathogen infiltration has explored extensively gut, little done study this phenomenon lower tract. In work, we hypothesize that synthetic bacterial...
ABSTRACT Engineered monoclonal antibodies (mAbs) have proven to be highly effective therapeutics in recent viral outbreaks due their specificity and ability provide immediate protection, regardless of immune status. However, despite technical advancements the field, an rapidly adapt or increase antibody affinity by extension, therapeutic efficacy, has yet fully realized. We endeavored stand-up such a pipeline using molecular modeling combined with experimental library screening given...
Abstract Neurotropic alphaviruses such as Venezuelan equine encephalitis virus (VEEV) are critical human pathogens that continually expand to naïve populations and for which there no licensed vaccines or therapeutics. VEEV is highly infectious via the aerosol route a recognized weaponizable biothreat causes neurological disease in humans. The neuropathology of has been attributed an inflammatory immune response brain yet underlying mechanisms specific cell involved not fully elucidated. This...
The emergence of drug-resistant pathogens necessitates the development new countermeasures. In this regard, introduction probiotics to directly attack or competitively exclude presents a useful strategy. Application approach requires an understanding how probiotic and its target pathogen interact. A key means probiotic-pathogen interaction involves production small molecules called natural products (NPs). Here, we report use whole-cell matrix-assisted laser desorption/ionization...
Abstract Rift Valley fever virus (RVFV) is a highly pathogenic mosquito-borne capable of causing hepatitis, encephalitis, blindness, hemorrhagic syndrome, and death in humans livestock. Upon aerosol infection with RVFV, the brain major site viral replication tissue damage, yet pathogenesis this organ has been understudied. Here, we investigated immune response RVFV infected mice. In to infection, microglia initiate robust transcriptional upregulation antiviral genes, as well increased levels...
Abstract The respiratory virus responsible for Coronavirus disease 2019 (COVID-19), Severe acute syndrome coronavirus 2 (SARS-2), has impacted nearly every aspect of life worldwide, claiming the lives over 2.5 million people globally, at time this publication. Neutralizing nanobodies (V H H) represent a promising therapeutic intervention strategy to address current SARS-2 pandemic and provide powerful toolkit future outbreaks. Using synthetic, high-diversity V bacteriophage library, several...