A5044490713- Hair Growth and Disorders
- Skin and Cellular Biology Research
- Wnt/β-catenin signaling in development and cancer
- Autoimmune Bullous Skin Diseases
- RNA regulation and disease
- Cell Adhesion Molecules Research
- melanin and skin pigmentation
- Immune Cell Function and Interaction
- Cellular Mechanics and Interactions
- Dermatology and Skin Diseases
- Pluripotent Stem Cells Research
- Dermatologic Treatments and Research
- Epigenetics and DNA Methylation
- Dermatological and Skeletal Disorders
- Cancer and Skin Lesions
- Genetic and rare skin diseases.
- Porphyrin Metabolism and Disorders
- Plant Reproductive Biology
- Connective tissue disorders research
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Wound Healing and Treatments
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Cytokine Signaling Pathways and Interactions
Columbia University Irving Medical Center
2016-2025
Columbia University
2015-2024
North American Hair Research Society
2019
Codarts Rotterdam
2016-2017
Council of Science Editors
2016-2017
Iowa City Public Library
2016
University Dermatology
1998-2016
Institut thématique Génétique, génomique et bioinformatique
2002-2013
National Alopecia Areata Foundation
2013
NewYork–Presbyterian Hospital
2010
There are several forms of hereditary human hair loss, known collectively as alopecias, the molecular bases which entirely unknown. A kindred with a rare, recessively inherited type alopecia universalis was used to search for locus by homozygosity mapping, and linkage established in 6-centimorgan interval on chromosome 8p12 (the logarithm odds favoring score 6.19). The homolog murine gene, hairless , localized this radiation hybrid missense mutation found affected individuals. Human encodes...
Significance Growth of de novo hair follicles in adult skin occurs by a process known as neogenesis. One way initiating neogenesis is to place dermal papillae isolated from the follicle contact with an overlying epidermis where they reprogram adopt follicular fate. This approach, however, has not been successful using cultured human papilla cells because lose their ability induce growth after expansion vitro. In this paper, we demonstrate that manipulating cell culture conditions establish...
BACKGROUND. Alopecia areata (AA) is an autoimmune disease characterized by hair loss mediated CD8+ T cells. There are no reliably effective therapies for AA. Based on recent developments in the understanding of pathomechanism AA, JAK inhibitors appear to be a therapeutic option; however, their efficacy treatment AA has not been systematically examined.
Embryonic stem cells (ESCs) have an unlimited proliferative capacity and extensive differentiation capability. They are alternative source for regenerative therapies with a potential role in the treatment of several human diseases. The clinical use ESCs, however, has significant ethical biological obstacles related to their derivation from embryos immunological rejection, respectively. These disadvantages can be circumvented by induced pluripotent (iPSCs), which generated individual's...
BACKGROUND. Alopecia areata (AA) is a common autoimmune disease with lifetime risk of 1.7%; there are no FDA-approved treatments for AA. We previously identified dominant IFN-γ transcriptional signature in cytotoxic T lymphocytes (CTLs) human and mouse AA skin showed that treatment JAK inhibitors induced durable hair regrowth mice by targeting this pathway. Here, we investigated the use oral JAK1/2 inhibitor ruxolitinib patients moderate-to-severe
Human skin constructs (HSCs) have the potential to provide an effective therapy for patients with significant injuries and enable human-relevant drug screening diseases; however, incorporation of engineered appendages, such as hair follicles (HFs), into HSCs remains a major challenge. Here, we demonstrate biomimetic approach generation human HFs within by recapitulating physiological 3D organization cells in HF microenvironment using 3D-printed molds. Overexpression Lef-1 dermal papilla...
Advances in bio-mimetic vitro human skin models increase the efficiency of drug screening studies. In this study, we designed and developed a microfluidic platform that allows for long-term maintenance full thickness equivalents (HSE) which are comprised both epidermal dermal compartments. The design is based on physiologically relevant blood residence times tissue establishment an air-epidermal interface crucial maturation terminal differentiation HSEs. small scale reduces amount culture...
Recent generation of patient-specific induced pluripotent stem cells (PS-iPSCs) provides significant advantages for cell- and gene-based therapy. Establishment iPSC-based therapy skin diseases requires efficient methodology differentiating iPSCs into both keratinocytes fibroblasts, the major cellular components skin, as well reconstruction structures using these iPSC-derived components. We previously reported from human use in treatment recessive dystrophic epidermolysis bullosa (RDEB)...
Alopecia areata (AA) is an autoimmune disease resulting in hair loss with devastating psychosocial consequences. Despite its high prevalence, there are no FDA-approved treatments for AA. Prior studies have identified a prominent interferon signature AA, which signals through JAK molecules.A patient AA was enrolled clinical trial to examine the efficacy of baricitinib, JAK1/2 inhibitor, treat concomitant CANDLE syndrome. In vivo, preclinical were conducted using C3H/HeJ mouse model assess...
Vascularization of engineered human skin constructs is crucial for recapitulation systemic drug delivery and their long-term survival, functionality, viable engraftment. In this study, the latest microfabrication techniques are used a novel bioengineering approach established to micropattern spatially controlled perfusable vascular networks in 3D equivalents using both primary induced pluripotent stem cell (iPSC)-derived endothelial cells. Using printing technology makes it possible control...
Several forms of hair loss in humans are characterized by the inability follicles to enter growth phase (anagen) cycle after being arrested resting (telogen). Current pharmacologic therapies have been largely unsuccessful targeting pathways that can be selectively modulated induce entry into anagen. We show topical treatment mouse and human skin with small-molecule inhibitors Janus kinase (JAK)-signal transducer activator transcription (STAT) pathway results rapid onset anagen subsequent...
Pseudoxanthoma elasticum (PXE), the prototypic heritable connective tissue disorder affecting elastic structures in body, manifests with cutaneous, ophthalmologic, and cardiovascular findings, considerable morbidity mortality. The molecular basis of PXE has remained unknown, but disease locus recently been mapped to an ≈500-kb interval on chromosome 16p13.1, without evidence for heterogeneity. In this study, we report pathogenetic mutations MRP6 , a member ABC transporter gene family, eight...