A5020018633- Spaceflight effects on biology
- Telomeres, Telomerase, and Senescence
- Neuroinflammation and Neurodegeneration Mechanisms
- HIV Research and Treatment
- Genetics, Aging, and Longevity in Model Organisms
- HIV/AIDS Research and Interventions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pregnancy-related medical research
- Gut microbiota and health
- Preterm Birth and Chorioamnionitis
- Cellular Mechanics and Interactions
- Genomics and Phylogenetic Studies
- Mosquito-borne diseases and control
- Alzheimer's disease research and treatments
- Reproductive Biology and Fertility
- Single-cell and spatial transcriptomics
- Pelvic floor disorders treatments
- Immune Cell Function and Interaction
- Pediatric Hepatobiliary Diseases and Treatments
- Marine Sponges and Natural Products
- Aerospace Engineering and Energy Systems
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Parvovirus B19 Infection Studies
- Extracellular vesicles in disease
- Diet and metabolism studies
Buck Institute for Research on Aging
2022-2025
Suzhou Municipal Hospital
2023-2025
Nanjing Medical University
2023-2025
Texas Biomedical Research Institute
2021-2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Zhongshan Hospital
2023
Fudan University
2023
Sun Yat-sen University
2023
Tulane University
2021-2022
Ocean University of China
2022
Spaceflight induces an immune response in astronauts. To better characterize this effect, we generated single-cell, multi-ome, cell-free RNA (cfRNA), biochemical, and hematology data for the SpaceX Inspiration4 (I4) mission crew. We found that 18 cytokines/chemokines related to inflammation, aging, muscle homeostasis changed after spaceflight. In I4 single-cell multi-omics data, identified a "spaceflight signature" of gene expression characterized by enrichment oxidative phosphorylation, UV...
Abstract Microgravity is associated with immunological dysfunction, though the mechanisms are poorly understood. Here, using single-cell analysis of human peripheral blood mononuclear cells (PBMCs) exposed to short term (25 hours) simulated microgravity, we characterize altered genes and pathways at basal stimulated states a Toll-like Receptor-7/8 agonist. We validate by RNA sequencing super-resolution microscopy, against data from Inspiration-4 (I4) mission, JAXA (Cell-Free Epigenome) Twins...
Abstract Telomeres are repetitive nucleoprotein complexes at chromosomal termini essential for maintaining genome stability. Telomeric RNA, or TERRA, is a previously presumed long noncoding RNA of heterogeneous lengths that contributes to end-capping structure and function, facilitates telomeric recombination in tumors maintain telomere length via the telomerase-independent Alternative Lengthening (ALT) pathway. Here, we investigated TERRA radiation-induced DNA damage response (DDR) across...
This study aimed to analyze the impact of preoperative cervical length before cerclage on extension gestational days in patients with various diagnostic types insufficiency, including obstetric history-based diagnosis, ultrasound-based and physical examination-based diagnosis. 168 were segregated into four categories based length: 0–0.4 cm, 0.5–1.4 1.5–2.4 ≥ 2.5 cm. Kaplan-Meier, linear regression curve Cox analyses used, a focus clinical variables, particularly duration after cerclage. The...
Abstract Background Appropriate preoperative identification of iCCA subtype is essential for personalized management, so the aim this study to investigate role MR imaging features in preoperatively differentiating subtype. Methods Ninety-three patients with mass-forming intrahepatic cholangiocarcinoma (iCCA, 63 small duct type and 30 large type) were retrospectively enrolled according latest 5th WHO classification (mean age, males vs. females: 60.66 ± 10.53 61.88 12.82, 50 men). Significant...
Cervical insufficiency is an increased risk of midterm miscarriage and early preterm birth which increase the fetal loss. This study aimed to construct a nomogram for patients with cervical after cerclage, may assist clinicians have individualized treatment insufficiency.A was done retrospectively from January 2013 through July 2022 in our hospital. The primary outcomes were delivered at more than 28, 30, 32, or 34 gestational weeks. Kaplan-Meier curves applied analyze 17 variables. All...
Abstract A dysregulated adaptive immune system is a key feature of aging, and associated with age-related chronic diseases mortality. Most notably, aging linked to loss in the diversity T cell repertoire expansion activated inflammatory subsets, though main drivers these processes are largely unknown. Here, we find that directly influenced by B cells. Using multiple models manipulation single-cell omics, cells be major type responsible for reduction naive cells, their differentiation towards...
Colostrum is well known to have excellent nutritional value for newborns. The aim of this study was investigate the dynamic expression pattern microRNA in human colostrum and mature milk. Furthermore, we identified specific analyzed regulatory function colostrum.We collected breast milk samples from 18 lactating volunteers. detected by microarray analysis. differences were characterized log2FC (|log2fold change| >1.58) associated P values (P<0.05). prediction targets, bioinformatics analysis...
Cells are subjected to dynamic mechanical environments which impart forces and induce cellular responses. In age-related conditions like pulmonary fibrosis, there is both an increase in tissue stiffness accumulation of senescent cells. While cells produce a senescence-associated secretory phenotype (SASP), the impact physical stimuli on senescence SASP not well understood. Here, we show that tension, modeled using cell culture substrate rigidity, influences markers SA-β-gal phenotypes....
The rebound-competent viral reservoir, composed of a virus that is able to persist during antiretroviral therapy (ART) and mediate reactivation systemic replication rebound viremia after ART interruption (ATI), remains the biggest obstacle treating HIV infection. A better understanding cellular tissue origins dynamics populations initiate upon ATI could help develop therapeutic strategies for reducing reservoir. In this study, barcoded simian immunodeficiency (SIV), SIVmac239M, was used...
The central nervous system (CNS) HIV reservoir is an obstacle to achieving cure. basal ganglia harbor a higher frequency of SIV than other brain regions in the SIV-infected rhesus macaques Chinese-origin (chRMs) even on suppressive combination antiretroviral therapy (ART). Since residual HIV/SIV associated with inflammation, we characterized neuroinflammation by gene expression and systemic levels inflammatory molecules healthy controls chRMs or without ART. CCL2, IL-6, IFN-γ were...
Abstract Ribonucleic acid (RNA)-seq data contain not only host transcriptomes but also nonhost information that comprises transcripts from active microbiota in the cells. Therefore, joint and integrative analyses of both meta-transcriptome can reveal gene expression microbial community a given sample as well correlative interactive dynamics response to microbiome. However, there are no convenient tools systemically analyze host–microbiota interactions through simultaneously quantifying same...
Abstract Background Antituberculosis-drug resistance is an important public health issue, and its epidemiological patterns has dramatically changed in recent decades. This study aimed to estimate the trends of multidrug-resistant tuberculosis (MDR-TB), which can be used inform strategies. Methods Data were collected from Global Burden Disease Study 2017. The estimated annual percentage changes (EAPCs) calculated assess MDR-TB burden at global, regional, national level 1990 Results Globally,...
Abstract The “gut-brain axis” is emerging as an important target in Alzheimer’s disease (AD). However, immunological mechanisms underlying this axis remain poorly understood. Using single-cell RNA sequencing of the colon immune compartment 5XFAD amyloid-β (Aβ) mouse model, we uncovered AD-associated changes ribosomal activity, oxidative stress, and BCR/plasma cell activity. Strikingly, levels CXCR4 + antibody secreting cells (ASCs) were significantly reduced. This corresponded with...
The "gut-brain axis" is emerging as an important target in Alzheimer's disease (AD). However, immunological mechanisms underlying this axis remain poorly understood. Using single-cell RNA sequencing of the colon immune compartment 5XFAD amyloid-β (Aβ) mouse model, we uncovered AD-associated changes ribosomal activity, oxidative stress, and BCR/plasma cell activity. Strikingly, levels CXCR4+ antibody secreting cells (ASCs) were significantly reduced. This corresponded with accumulating B...
A major aging hallmark is the accumulation of cellular senescence burden. Over time senescent cells contribute to tissue deterioration through chronic inflammation and fibrosis driven by Senescence-Associated Secretory Phenotype (SASP). The human ovary one first organs age, prominent age-related fibroinflammation within ovarian microenvironment consistent with presence cells, but these have not been characterized in ovary. We thus established a doxorubicin-induced model establish senotype...
To study the distribution characteristics of lactobacillus in vaginal mucosa patients with HPV infection.The planting density secretions 95 cases HPV16/18 infection and 90 normal women childbearing age were observed by oil microscope. And strains lactobacilli two groups analyzed using species-specific polymerase chain reaction (Species-specific PCR) investigated.In infective groups, was 104 (68-186)/HP. It significantly lower than that group (234 (161-326)/HP, P<0.05). Compared group,...
Abstract The rebound-competent viral reservoir (RCVR), comprised of virus that is able to persist during antiretroviral therapy (ART) and mediate reactivation systemic replication rebound viremia after interruption (ATI), remains the biggest obstacle eradication HIV infection. A better understanding cellular tissue origins dynamics populations initiate upon ATI could help develop targeted therapeutic strategies for reducing RCVR. In this study, barcoded SIVmac239M was used infect rhesus...