A5015231927- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Spaceflight effects on biology
- Electrochemical Analysis and Applications
- DNA and Nucleic Acid Chemistry
- Photoreceptor and optogenetics research
- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Light effects on plants
- Soil and Unsaturated Flow
- Landslides and related hazards
- T-cell and B-cell Immunology
- High Altitude and Hypoxia
- Micro and Nano Robotics
- Machine Learning in Healthcare
- Tryptophan and brain disorders
- Porphyrin Metabolism and Disorders
- Atherosclerosis and Cardiovascular Diseases
- Aerospace Engineering and Energy Systems
- Auditing, Earnings Management, Governance
- Advanced Cellulose Research Studies
- Quantum Dots Synthesis And Properties
- Immune Cell Function and Interaction
- Enzyme Catalysis and Immobilization
- Hemostasis and retained surgical items
Buck Institute for Research on Aging
2021-2024
Vietnam National University Ho Chi Minh City
2024
Ho Chi Minh City International University
2023
Hai Phong University
2023
Trường ĐH Nguyễn Tất Thành
2017-2022
Duy Tan University
2016-2020
University of Utah
2007-2017
Salt Lake Regional Medical Center
2015
Institute of Medicinal Plant Development
2009
Institut Rayonnement-Matière de Saclay
2008
Spaceflight induces an immune response in astronauts. To better characterize this effect, we generated single-cell, multi-ome, cell-free RNA (cfRNA), biochemical, and hematology data for the SpaceX Inspiration4 (I4) mission crew. We found that 18 cytokines/chemokines related to inflammation, aging, muscle homeostasis changed after spaceflight. In I4 single-cell multi-omics data, identified a "spaceflight signature" of gene expression characterized by enrichment oxidative phosphorylation, UV...
Abstract Microgravity is associated with immunological dysfunction, though the mechanisms are poorly understood. Here, using single-cell analysis of human peripheral blood mononuclear cells (PBMCs) exposed to short term (25 hours) simulated microgravity, we characterize altered genes and pathways at basal stimulated states a Toll-like Receptor-7/8 agonist. We validate by RNA sequencing super-resolution microscopy, against data from Inspiration-4 (I4) mission, JAXA (Cell-Free Epigenome) Twins...
We present here the construction of a motion-based DNA sensor that only produces motion signal in presence target.
Significance UV photons are absorbed strongly by DNA, but rarely cause permanent photodamage. Single nucleobases protected ultrafast nonradiative decay, excited states in single- and double-stranded DNA decay very differently. An intensely debated question is whether a photon can move an electron from one nucleobase to another along single strand. This study demonstrates that absorption efficiently transfers oxidatively damaged guanine (8-oxo-G) adenine dinucleotide mimic of the flavin...
Glycosaminoglycan (GAG) biosynthesis requires numerous biosynthetic enzymes and activated sulfate sugar donors. Although the sequence of events is resolved using reconstituted systems, little known about emergence cell-specific GAG chains (heparan sulfate, chondroitin dermatan sulfate) with distinct sulfation patterns. We have utilized a library click-xylosides that various aglycones to decipher mechanism in cellular system. Earlier studies shown both concentration primers structure aglycone...
Redox-active enzyme cofactors derived from ribonucleotides have been called "fossils of the RNA world," suggesting that early catalysts employed modified nucleobases to facilitate redox chemistry in primitive metabolism. Here, we show common oxidative damage product 8-oxo-7,8-dihydroguanine (OG), when incorporated into a DNA or strand proximity cyclobutane pyrimidine dimer, can mimic function flavin photorepair. The OG nucleotide acts catalytically mechanism consistent with photolyase which...
Aging is characterized by a progressive loss of brain volume at an estimated rate 5% per decade after age 40. While these morphometric changes, especially those affecting gray matter and atrophy the temporal lobe, are predictors cognitive performance, strong association with aging obscures potential parallel, but more specific role, individual subject physiology. Here, we studied cohort 554 human subjects who were monitored using structural MRI scans blood immune protein concentrations....
Purpose This study aims to investigate the relationship between corporate tax avoidance and stock price crash risk moderating effects of governance. Design/methodology/approach investigates using sample consisting listed firms in Vietnam for period 2011–2020 panel regressions. Findings The authors find that there is a positive risk. Foreign ownership weakens impacts on risk, while managerial strengthens impacts. Female Chief Executive Officers (CEOs) female chairpersons weaken this...
Femtosecond time-resolved IR spectroscopy is used to investigate the excited-state dynamics of a dinucleotide containing an 8-oxoguanine anion at 5'-end and neutral adenine 3'-end. UV excitation transfers electron from deprotonated its π-stacked neighbor in less than 1 ps, generating radical anion. These species are identified by excellent agreement between experimental calculated difference spectra. The quantum efficiency this ultrafast charge shift reaction approaches unity. Back transfer...
We present the preparation of a redox DNA hydrogel for mediated bioelectrocatalysis oxidoreductase enzymes biosensor and biofuel cell applications. The noncovalent functionalization with molecules is achieved by intercalation aromatic probes into double helix or electrostatic binding redox-active tetraalkylammonium ions to phosphate groups on DNA. Prepared hydrogels demonstrate capability mediating bioelectrocatalytic glucose oxidation enzymes. This first evidence that can replace polymer...
The development of enzymatic biofuel cells has been plagued by the high cost enzyme purification and low efficiency fuel oxidation. Here, we demonstrate a protein purification-free approach to assemble an alcohol dehydrogenase aldehyde cascade-based bioanode for use in methanol cell. Each was fused different sequence-specific zinc finger DNA-binding protein. domains serve as both tags isolate enzymes from crude cell lysates well anchors immobilize on DNA-modified multiwalled carbon...
The employment of multi-enzyme cascades as catalysts is a promising strategy to improve performance enzymatic biofuel cells, it allows for the conversion more chemical energy stored in complex fuels. However, these enzyme systems often limited by mass transport intermediate substrates between enzymes. Nature addresses this issue organizing metabolic enzymes sequential and proximal manner enhance efficiency pathways. In work, we investigate utilization DNA structural scaffold assembly...
Present-day organisms are under constant environmental stress that damages bases in DNA, leading to mutations. Without DNA repair processes correct these errors, such damage would be catastrophic. Organisms all kingdoms have ranging from direct reversal base excision and nucleotide repair, the recently characterized giant viruses also include mechanisms. At what point evolution of genomes did active mechanisms become critical? In particular, how early RNA protect themselves UV photodamage...
DNA hydrogels as a method for enzyme entrapment in an enzymatic biobattery.
A good GAG. In order to modulate glycosaminoglycan (GAG) biosynthesis, and determine their physiological developmental role in a spatiotemporal manner, we synthesized library of “click” xylosides with various aglycone moieties that are very stable under conditions. The compounds were examined for ability stimulate GAG chains cell line is defective the production chains.
Abstract A dysregulated adaptive immune system is a key feature of aging, and associated with age-related chronic diseases mortality. Most notably, aging linked to loss in the diversity T cell repertoire expansion activated inflammatory subsets, though main drivers these processes are largely unknown. Here, we find that directly influenced by B cells. Using multiple models manipulation single-cell omics, cells be major type responsible for reduction naive cells, their differentiation towards...
The 8‐oxopurine nucleosides 2′,3′,5′‐tri‐ O ‐acetyl‐8‐oxo‐7,8‐dihydroguanosine (OG) and ‐acetyl‐ribosyluric acid (RU) were studied for their ability to mediate the photochemical (λ > 300 nm) reversion of cyclobutane pyrimidine dimers parent pyrimidines thymine uracil. bimolecular reactions these monomers proceeded at very slow rates compared with recently published work using oligonucleotide contexts; nevertheless, it was possible make comparisons between efficacy OG RU as photocatalysts...
The correlation between the soil compression index (Cc) and state parameters is frequently referenced in studies investigating fundamental mechanisms underlying changes compressibility. However, developing a universal formula for Cc that adequately captures complexity of compressive behavior has been challenging conventional approaches. This study leveraged contemporary symbolic regression (SR) to propose generalized can represents nonlinear relationships with various parameters. A...