A biomaterial-based vaccination system that uses minimal extracorporeal manipulation could provide in situ enhancement of dendritic cell (DC) numbers, a physical space where DCs interface with transplanted tumour cells, and an immunogenic context. Here we encapsulate GM-CSF, serving as DC factor, CpG ODN, activating into sponge-like macroporous cryogels. These cryogels are injected subcutaneously mice to localize cells deliver immunomodulatory factors controlled spatio-temporal manner....

An optimized 3D inkjet printing process is demonstrated for structuring alginate into a tissue-like microvasculature capable of supporting physiological flow rates. Optimizing the reaction at single-droplet level enables wet hydrogel droplets to be stacked, thus overcoming their natural tendancy spread and coalesce. Live cells can patterned using this it extended range other hydrogels.

Using the concept of insulator-based "electrodeless" dielectrophoresis, we present a novel geometry for shaping electric fields to achieve lateral deviation particles in liquid flows. The field is generated by planar metal electrodes and guided along access channels active area main channel. equipotential surfaces at apertures behave as vertical "liquid" injecting current into between pair adjacent generates dielectrophoretic force necessary particle manipulation. We use this high-speed...

We present a particle-sorting device based on the opposition of dielectrophoretic forces. The forces are generated by an array electrode chambers located in both sidewalls main flow channel. Particles with different dielectric response perceive force magnitudes and therefore continuously focused to streamlines relate particles' their output position downstream demonstrate performance separating mixed yeast cell population into pure fractions viable nonviable cells. Finally, we use enrich red...

Millimeter to centimeter‐sized injectable neural scaffolds based on macroporous cryogels are presented. The polymer‐scaffolds made from alginate and carboxymethyl‐cellulose by a novel simple one‐pot cryosynthesis. They allow surgical sterility means of autoclaving, present native laminin as an attachment motive for adhesion neurite development. designed protect extended, living neuronal network during compression small fraction the original volume in order enable minimally invasive delivery....

Delayed, large-volume bacterial culture and amotosalen/ultraviolet-A light pathogen reduction are effective at reducing the risk of proliferation in platelet concentrates (PCs). Hemovigilance programs continue to receive reports suspected septic transfusion reactions, most with low imputability. Here, we compile national hemovigilance data determine relative efficacy these interventions.Annual from United Kingdom, France, Switzerland, Belgium were reviewed between 2005 2016 assess...

Microfluidic devices are increasingly used to perform biological experiments on a single-cell basis. However, long-term stability of cell positions is still an issue. A novel biocompatible method for entrapment and release microchip presented. It based the controlled formation alginate hydrogel by bringing two laminar flows calcium ions in range 2 mM 40 into contact. The resulting growth gel bar enclose immobilize yeast cells. Adding ethylenediaminetetraacetic acid (EDTA) solution allows...

There is great interest in highly sensitive separation methods capable of quickly isolating a particular cell type within single manipulation step prior to their analysis. We present sorting device based on the opposition dielectrophoretic forces that discriminates between types according dielectric properties, such as membrane permittivity and cytoplasm conductivity. The are generated by an array electrodes located both sidewalls main flow channel. Cells with different responses perceive...

We present a device capable of electrical cell lysis and evaluation efficiency in continuous flow using dielectrophoretic sorting. use combination AC fields so-called liquid electrodes to avoid bubble creation at the electrode surface. The field distribution is calculated different configurations by numerical simulations. Cell sorting shows high efficiency, 99% yeast cells sorted after featuring dielectric properties similar dead cells. A study potential throughput performed.

Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of decellularized extracellular matrix (dECM) from porcine ventricular fibrinogen that is suitable for investigations employing an in vitro 3D cell culture model. Rapid specific coagulation with thrombin facilitates the gentle inclusion cells while avoiding sedimentation during formation dECM-fibrin composite. Our revealed enhanced cardiogenic differentiation H9c2 myoblast when...

Cardiomyocyte alignment in myocardium tissue plays a significant role the physiological, electrical, and mechanical functions of myocardium. It remains, however, difficult to align cardiac cells 3D vitro heart model. This paper proposes simple method using microfabricated Polydimethylsiloxane (PDMS) grooves with large dimensions (of up 350 µm width), similar trabeculae carneae, smallest functional unit Two cell groups were used this work; first, H9c2 combination Nor10 for proof concept,...

We present a microfluidic device where micro- and nanoparticles can be continuously functionalized in flow. This relies on an element called "particle exchanger", which allows for particles to taken from one medium exposed some reagent while minimizing mixing of the two liquids. In exchanger, liquids are brought contact pushed other by application dielectrophoretic force. determined maximum flow velocity at all exchanged range particle sizes. also simple theory that accounts behaviour when...

We provide a common theoretical framework reuniting specific models for the Ca(2+)-alginate system and general reaction diffusion theory along with experimental validation on microfluidic chip. As starting point, we use set of nonlinear, partial differential equations that are traditionally solved numerically: Mikkelsen-Elgsaeter model. Applying traveling-wave hypothesis as major simplification, obtain an analytical solution. The solution indicates fundamental properties alginate front...

Recently, 3D printing has become a promising technique in biomedical engineering applications. These techniques have undergone numerous changes since they were first produced, from the instrument to material used. Herein, we provided an overview and comprehensive insight into rapidly progressing field of additive manufacturing, called lithography-based printing. This been topic interest past decade, when potential irradiated light emerged tissue engineering. At first, significant differences...

We present a miniaturized impedance imaging system, developed for 2D of cell and tissue culture. The system is based on 16 microelectrodes (5 microm x 4 mm). An equivalent circuit four-point (tetrapolar) spectra was validated. uses an Agilent 4294A analyser combined with front-end amplifier the measurements. Human epithelial stem cells (YF 29) were grown device surface. Cell migration speeds 300 nm min(-1) following "scratch" wound closure assay could be established. Using commercial...

Abstract A novel type of injectable biomaterial with an elastic softening transition is described. The material enables in vivo shaping, followed by induction 3D stable vascularized tissue. synthesis the meta‐biomaterial instructed extensive numerical simulation as a suspension irregularly fragmented, highly porous sponge‐like microgels. irregular particle shape dramatically enhances yield strain for stability against deformation. Porosity particles, along friction between internal surfaces,...

Cell cycle synchronization is an important tool for the study of cell division stages and signalling. It provides homogeneous cultures that are importance to develop improve processes such as protein synthesis drug screening. The main approach today use metabolic agents block at a particular phase accumulate cells this phase, disturbing physiology. We provide here non-invasive label-free continuous sorting technique analyze synchronize yeast division. By balancing opposing dielectrophoretic...

Abstract Cryogels are macroporous materials that display remarkable properties, such as high pore interconnection, large surface to volume ratio, and mechanical stability, making them good candidates for 3D cell culture. However, shaping cryogels remains challenging because of the harsh conditions synthesis at temperatures low −80 °C. In this paper, a solution printing functionalized is proposed. A microfabricated dispensing probe allowing last second mixing cryogel precursors well control...