A5076097847- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Immune cells in cancer
- Wound Healing and Treatments
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Cellular Mechanics and Interactions
- Spine and Intervertebral Disc Pathology
- CAR-T cell therapy research
- Biomedical Ethics and Regulation
- Hydrogels: synthesis, properties, applications
- Spaceflight effects on biology
- Radiation Therapy and Dosimetry
- Nanoplatforms for cancer theranostics
- Surgical Sutures and Adhesives
- Biomedical and Engineering Education
- Monoclonal and Polyclonal Antibodies Research
- Bone Metabolism and Diseases
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
Columbia University
2021-2024
Columbia University Irving Medical Center
2024
Cornell University
2019-2022
Drexel University
2014-2020
The University of Sydney
2017
Centenary Institute
2017
Macrophages are key contributors to vascularization, but the mechanisms behind their actions not understood. Here, we show that diverse macrophage phenotypes have distinct effects on endothelial cell behavior, with resulting vascularization of engineered tissues. In Transwell coculture, proinflammatory M1 macrophages caused cells up-regulate genes associated sprouting angiogenesis, whereas prohealing (M2a), proremodeling (M2c), and anti-inflammatory (M2f) promoted up-regulation pericyte...
Chronic wounds remain a major burden to the global healthcare system. Myriad wound matrices are commercially available but their mechanisms of action poorly understood. Recent studies have shown that macrophages highly influenced by microenvironment, it is not known how different biomaterials affect this interaction. Here, was hypothesized human respond differently changes in biomaterial properties vitro with respect phenotype, including pro-inflammatory M1, anti-inflammatory M2a, for...
Abstract Galactic cosmic radiation (GCR) is one of the most serious risks posed to astronauts during missions Moon and Mars. Experimental models capable recapitulating human physiology are critical understanding effects on organs developing radioprotective measures against space travel exposures. The systemic studied using a multi‐organ‐on‐a‐chip (multi‐OoC) platform containing engineered tissue bone marrow (site hematopoiesis acute damage), cardiac muscle chronic damage) liver metabolism),...
Abstract The topography of a biomaterial regulates cellular interactions and determine stem cell fate. A complete understanding how topographical properties affect behavior will allow the rational design material surfaces that elicit specified biological functions once placed in body. To this end, we fabricate substrates with aligned or randomly organized fibrous nanostructured topographies. Culturing adipose-derived cells (ASCs), explore dynamic relationship between alignment topography,...
Macrophages, the primary cells of inflammatory response, are major regulators healing, and mediate both bone fracture healing response to implanted biomaterials. However, their phenotypic contributions biomaterial-mediated repair incompletely understood. Therefore, we used gene expression protein secretion analysis investigate interactions in vitro between human monocyte-derived macrophages ceramic scaffolds that have been shown varying degrees success promoting regeneration vivo...
Resident cardiac macrophages are critical mediators of function. Despite their known importance to electrophysiology and tissue maintenance, there currently no stem-cell-derived models human engineered tissues (hECTs) that include resident macrophages. In this study, we made an induced pluripotent stem cell (iPSC)-derived hECT model with a population (iM0) better recapitulate the native myocardium characterized impact on Macrophage retention within hECTs was confirmed via immunofluorescence...
Systemic lupus erythematosus (SLE) is a heterogenous autoimmune disease that affects multiple organs, including the heart. The mechanisms of myocardial injury in SLE remain poorly understood. In this study, we engineered human cardiac tissues and cultured them with IgG from patients SLE, without involvement. elevated inflammation exhibited increased binding to apoptotic cells within subjected stress, whereas systolic dysfunction enhanced surface live cardiomyocytes. Functional assays RNA...
Incomplete understanding of metastatic disease mechanisms continues to hinder effective treatment cancer. Despite remarkable advancements toward the identification druggable targets, options for patients in remission following primary tumor resection remain limited. Bioengineered human tissue models sites capable recreating physiologically relevant milieu colonization may strengthen our grasp cancer progression and contribute development therapeutic strategies. We report use an engineered...
Abstract Antibiotic‐resistant bacteria are a major global health threat that continues to rise due lack of effective vaccines. Of concern Klebsiella pneumoniae ( K. ) fail induce in vivo germinal center B cell responses, which facilitate antibody production fight infection. Immunotherapies using antibodies targeting antibiotic‐resistant emerging as promising alternatives, however, they cannot be efficiently derived ex vivo, necessitating the need for immune technologies develop therapeutics....
Abstract Pulmonary air leak is the most common complication of lung surgery, contributing to post‐operative morbidity in up 60% patients; yet, there no reliable treatment. Available surgical sealants do not match demanding deformation mechanics tissue; and therefore, fail seal leak. To address this therapeutic gap, a sealant with structural mechanical similarity subpleural designed, developed, systematically evaluated. This “lung‐mimetic” hydrofoam material that has alveolar‐like porous...
Macrophages play an important role in tissue repair, regeneration, and the ability of biomaterials to mediate these processes. are highly plastic cells that exhibit altered behavior response changes microenvironment. With growing knowledge roles different macrophage phenotypes specific pathologies and/or injuries, researchers now focusing on designing actively control promote healing outcomes. In this review, we highlight a variety biomaterial strategies for controlling phenotype chronic...
Memory B cells originate in response to antigenic stimulation B-cell follicles of secondary lymphoid organs where naive undergo maturation within a subanatomical microenvironment, the germinal centers. The understanding memory immunology and its regulation is based primarily on sophisticated experiments that involve mouse models. To date, limited evidence exists whether can be successfully engineered ex vivo, specifically using biomaterials-based platforms support growth differentiation...
Injectable biomaterials are defined as implantable materials that can be introduced into the body a liquid and solidify in situ. Such offer clinical advantages of being implanted minimally invasively easily forming space-filling solids irregularly shaped defects. have been widely investigated scaffolds for tissue engineering. However, repair certain load-bearing areas body, such intervertebral disc, should possess adhesive properties. This will minimize risk dislocation during motion ensure...
Injectable biomaterials are defined as implantable materials that can be introduced into the body a liquid and solidify in situ. Such offer clinical advantages of being implanted minimally invasively easily forming space-filling solids irregularly shaped defects. have been widely investigated scaffolds for tissue engineering. However, repair certain load-bearing areas body, such intervertebral disc, should possess adhesive properties. This will minimize risk dislocation during motion ensure...
Systemic lupus erythematosus (SLE) is a highly heterogenous autoimmune disease that affects multiple organs, including the heart. The mechanisms by which myocardial injury develops in SLE, however, remain poorly understood. Here we engineered human cardiac tissues and cultured them with IgG fractions containing autoantibodies from SLE patients without involvement. We observed unique binding patterns of two patient subgroups: (i) severe inflammation exhibited enhanced to apoptotic cells...