A5032764850- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Neuroscience and Neural Engineering
- Cardiac electrophysiology and arrhythmias
- Systemic Lupus Erythematosus Research
- Electrospun Nanofibers in Biomedical Applications
- Spaceflight effects on biology
- Atherosclerosis and Cardiovascular Diseases
- Pluripotent Stem Cells Research
- Probiotics and Fermented Foods
- Planarian Biology and Electrostimulation
- Cardiac Structural Anomalies and Repair
- Monoclonal and Polyclonal Antibodies Research
- Cardiovascular Function and Risk Factors
- Radiation Therapy and Dosimetry
- CRISPR and Genetic Engineering
- Signaling Pathways in Disease
- Extracellular vesicles in disease
- Receptor Mechanisms and Signaling
- Peptidase Inhibition and Analysis
- Congenital heart defects research
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Fuel Cells and Related Materials
- COVID-19 Clinical Research Studies
Columbia University
2020-2024
Columbia University Irving Medical Center
2022
Royal Museums Greenwich
2022
Oregon State University
2019
Light is necessary for life, but prolonged exposure to artificial light a matter of increasing health concern. Humans are exposed increased amounts in the blue spectrum produced by light-emitting diodes (LEDs), which can interfere with normal sleep cycles. The LED technologies relatively new; therefore, long-term effects across lifespan not understood. We investigated model organism, Drosophila melanogaster, and determined that flies maintained daily cycles 12-h darkness had significantly...
Epicardial cells (EPIs) form the outer layer of heart and play an important role in development disease. Current heart-on-a-chip platforms still do not fully mimic native cardiac environment due to absence relevant cell types, such as EPIs. Here, using Biowire II platform, engineered tissues with epicardial inner myocardial structure are constructed, image analysis approach is developed track EPI migration a beating environment. Functional properties improve over two weeks culture. In...
Abstract Galactic cosmic radiation (GCR) is one of the most serious risks posed to astronauts during missions Moon and Mars. Experimental models capable recapitulating human physiology are critical understanding effects on organs developing radioprotective measures against space travel exposures. The systemic studied using a multi‐organ‐on‐a‐chip (multi‐OoC) platform containing engineered tissue bone marrow (site hematopoiesis acute damage), cardiac muscle chronic damage) liver metabolism),...
Resident cardiac macrophages are critical mediators of function. Despite their known importance to electrophysiology and tissue maintenance, there currently no stem-cell-derived models human engineered tissues (hECTs) that include resident macrophages. In this study, we made an induced pluripotent stem cell (iPSC)-derived hECT model with a population (iM0) better recapitulate the native myocardium characterized impact on Macrophage retention within hECTs was confirmed via immunofluorescence...
Engineered cardiac tissues derived from human induced pluripotent stem cells (iPSCs) are increasingly used for drug discovery, pharmacology and in models of development disease. While there numerous platforms to engineer tissues, they often require expensive nonconventional equipment utilize complex video-processing algorithms. As a result, only specialized academic laboratories have been able harness this technology. In addition, methodologies tissue features challenging reproduce between...
Restrictive cardiomyopathy (RCM) is defined as increased myocardial stiffness and impaired diastolic relaxation leading to elevated ventricular filling pressures. Human variants in filamin C (FLNC) are linked a variety of cardiomyopathies, this study, we investigate an in-frame deletion (c.7416_7418delGAA, p.Glu2472_Asn2473delinAsp) patient with RCM. Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) variant display reduced calcium kinetics 2D culture when compared...
Heart failure (HF) is characterized by global alterations in myocardial DNA methylation, yet little known about the epigenetic regulation of noncoding genome and potential reversibility methylation with left ventricular assist device (LVAD) therapy. Genome-wide mapping 36 patients HF at LVAD implantation, 8 explantation, 7 nonfailing (NF) donors using a high-density bead array platform identified 2,079 differentially methylated positions (DMPs) ischemic cardiomyopathy (ICM) 261 DMPs...
Systemic lupus erythematosus (SLE) is a heterogenous autoimmune disease that affects multiple organs, including the heart. The mechanisms of myocardial injury in SLE remain poorly understood. In this study, we engineered human cardiac tissues and cultured them with IgG from patients SLE, without involvement. elevated inflammation exhibited increased binding to apoptotic cells within subjected stress, whereas systolic dysfunction enhanced surface live cardiomyocytes. Functional assays RNA...
Extracellular appendages play a significant role in mediating communication between bacteria and their host. Curli fibers are class of bacterial fimbria that is highly amenable to engineering. We demonstrate the use engineered curli rationally program interactions components mucosal epithelium. Commensal E. coli strains were produce recombinant fused trefoil family human cytokines. Biofilms formed from these bound more mucins than those producing wild-type fibers, modulated mucin rheology as...
<italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Goal:</i> Contractile response and calcium handling are central to understanding cardiac function physiology, yet existing methods of analysis quantify these metrics often time-consuming, prone mistakes, or require specialized equipment/license. We developed BeatProfiler, a suite tools designed contractile function, handling, force generation for multiple in vitro models apply downstream machine...
Adverse cardiac outcomes in COVID-19 patients, particularly those with preexisting disease, motivate the development of human cell-based organ-on-a-chip models to recapitulate injury and dysfunction for screening cardioprotective therapeutics. Here, we developed a heart-on-a-chip model study pathogenesis SARS-CoV-2 healthy myocardium established from induced pluripotent stem cell (iPSC)-derived cardiomyocytes model, mimicking aspects hypertensive disease by angiotensin II (Ang II). We...
In the heart, protein kinase A (PKA) is critical for activating calcium handling and sarcomeric proteins in response to beta-adrenergic stimulation leading increased myocardial contractility performance. The catalytic activity of PKA tightly regulated by regulatory subunits that inhibit subunit until released cAMP binding. Phosphorylation type II promotes activation; however, role phosphorylation I remain uncertain. Here, we utilize human induced pluripotent stem cell cardiomyocytes...
Systemic lupus erythematosus (SLE) is a highly heterogenous autoimmune disease that affects multiple organs, including the heart. The mechanisms by which myocardial injury develops in SLE, however, remain poorly understood. Here we engineered human cardiac tissues and cultured them with IgG fractions containing autoantibodies from SLE patients without involvement. We observed unique binding patterns of two patient subgroups: (i) severe inflammation exhibited enhanced to apoptotic cells...
Abstract Background Human heart failure is characterized by global alterations in the myocardial DNA methylation profile, yet little known about epigenetic regulation of non-coding transcripts and potential reversibility with left ventricular assist device (LVAD) support. Method Genome-wide mapping was performed 36 patients end-stage at time LVAD implant, 8 explant, 7 non-failing controls using high-density bead array platform. Transcriptomic functional studies were human induced pluripotent...
Many neuromuscular diseases, such as myasthenia gravis (MG), are associated with dysfunction of the junction (NMJ), which is difficult to characterize in animal models due physiological differences between animals and humans. Tissue engineering offers opportunities provide vitro functional human NMJs that can be used diagnose investigate NMJ pathologies test potential therapeutics. By incorporating optogenetic proteins into induced pluripotent stem cells (iPSCs), we generated neurons...
Introduction: Dysregulation of cardiac kinases occurs in cardiovascular diseases including hypertrophy, remodeling and ischemia injury. Serine/threonine protein kinase 25 (STK25) is a stress response putative tumor suppressor that regulates cell survival metabolism through several signaling pathways, LATS/3HIPPO mTOR pathways. Hypothesis: STK25 survival, function human cardiomyocytes animal injury models. Methods: CRISPR-Cas9 was used to generate homozygous knockout (KO) induced pluripotent...
A considerable number of patients with systemic lupus erythematosus (SLE) develop myocardial injury. SLE have highly heterogenous populations autoantibodies that may contribute to cardiac disease pathogenesis and heterogeneity; however, little is known about their direct role in Here, we engineered a model matured human tissue used it study induced To this end, cultured tissues immunoglobulin G (IgG) from without We found IgG binding levels differed between patient groups correlated measures...
In the native human myocardium, cardiac resident macrophages are a critical mediator for normal function, performing variety of biological roles in electrical conduction, regeneration, and phagocytosis efferocytosis. However, most models engineered tissues (hECT), which aim to recapitulate adult myocardium disease modeling drug screening applications, do not include macrophage population. Thus, this study we introduced an iPSC-derived (iM0) population into all tissue model order assess its...