A5016270089- Acute Kidney Injury Research
- Circular RNAs in diseases
- Chronic Kidney Disease and Diabetes
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- Immune Response and Inflammation
- Osteoarthritis Treatment and Mechanisms
- Colorectal Cancer Screening and Detection
- Sepsis Diagnosis and Treatment
- Extracellular vesicles in disease
- Cardiac electrophysiology and arrhythmias
- Biomarkers in Disease Mechanisms
- Cancer-related molecular mechanisms research
- Urinary and Genital Oncology Studies
- Ubiquitin and proteasome pathways
- Bladder and Urothelial Cancer Treatments
- Parkinson's Disease Mechanisms and Treatments
- Histone Deacetylase Inhibitors Research
- Signaling Pathways in Disease
- Receptor Mechanisms and Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Diversity and Health Studies
- Congenital heart defects research
- Alzheimer's disease research and treatments
- Lipid metabolism and biosynthesis
Hunan Normal University
2021-2024
North China University of Science and Technology
2024
Sun Yat-sen University
2023-2024
Peking University
2020-2024
Third Affiliated Hospital of Sun Yat-sen University
2024
Central South University
2018-2023
Sun Yat-sen Memorial Hospital
2023
Second Xiangya Hospital of Central South University
2018-2023
Northeast Agricultural University
2022-2023
Wuhan University
2023
Recent studies have reported that upregulation of disulfide-bond A oxidoreductase-like protein (DsbA-L) prevented lipid-induced renal injury in diabetic nephropathy (DN). However, the role and regulation proximal tubular DsbA-L for tubulointerstitial fibrosis (TIF) remains unclear. In current study, we found a tubules-specific knockout mouse (PT-DsbA-L-KO) attenuated UUO-induced TIF, cell apoptosis inflammation. Mechanistically, interacted with Hsp90 mitochondria BUMPT cells which activated...
Abstract Abnormal activation of the oncogene YAP in Hippo pathway is a major feature liver cancer and inactivation MST1/2 has been shown to be responsible for overactivation that led tumorigenesis. However, mechanisms underlying dysregulation remain poorly understood. RNA‐seq analysis genome (KEGG) enrichment were used identify genes pathways regulated by SIRT7. qRT‐PCR, ChIP, luciferase assay investigate transcriptional regulation. Mass spectrometry, co‐immunoprecipitation...
Abstract Non-resolved persistent macrophage-mediated synovial inflammation is considered as one of the main drivers both establishment and progression obesity-associated osteoarthritis (OA). Herein, we used clodronate-loaded liposomes (CL) to locally deplete macrophages in joints examine role obesity-induced OA. Furthermore, resolvin D1 (RvD1), a unique family pro-resolving lipid mediator derived from omega-3 polyunsaturated fatty acid, have shown marked potency changing pro-inflammatory...
The prediction of mortality for septic acute kidney injury (AKI) has been assessed by a number potential biomarkers, including long noncoding RNAs (lncRNAs). However, the validation lncRNAs as particularly early stages AKI, is still warranted. Our results indicate that lncRNA TCONS_00016233 upregulated in plasma sepsis-associated non-AKI and AKI patients, but higher cutoff threshold (9.5 × 105, copy number) provided sensitivity 71.9% specificity 89.6% detection AKI. was highly correlated...
Under stress conditions, translationally stalled mRNA and associated proteins undergo liquid-liquid phase separation condense into cytoplasmic foci called granules (SGs). Many viruses hijack SGs for their pathogenesis; however, whether pathogenic bacteria also exploit this pathway remains unknown. Here, we report that members of the OspC family Shigella flexneri induce SG formation in infected cells. Mechanistically, effectors target multiple subunits host translation initiation factor 3...
BackgroundUrine cytology is an important non-invasive examination for urothelial carcinoma (UC) diagnosis and follow-up. We aimed to explore whether artificial intelligence (AI) can enhance the sensitivity of urine help avoid unnecessary endoscopy.MethodsIn this multicentre diagnostic study, consecutive patients who underwent liquid-based examinations at four hospitals in China were included model development validation. Patients declined surgery lacked associated histopathology results,...
Our previous study demonstrated that the methyl-CpG-binding domain protein 2 (MBD2) mediates vancomycin (VAN)-induced acute kidney injury (AKI). However, role and regulation of MBD2 in septic AKI are unknown. Herein, was induced by lipopolysaccharide (LPS) Boston University mouse proximal tubules (BUMPTs) mice. For both vitro vivo experiments, we showed inhibition small interfering RNA (siRNA) MBD2-knockout (KO) substantially improved survival rate attenuated LPS cecal ligation puncture...
Abstract Previous studies reported that RAGE participated in the process of kidney fibrosis, but function and regulation pathway proximal tubular cells this remains unclear. Here, we found expression was increased by TGF-β1 treatment unilateral ureteral obstruction (UUO). Knock down ameliorated renal fibrosis treatment, vimentin, Collagen I&III, fibronectin are decreased. Mechanistically, mediated TGF-β1-induced phosphorylation Stat3 directly upregulated Atg7 to increase level autophagy,...
Gout is an oxidative stress-related disease. Food-derived vanillic acid, a promising xanthine oxidase inhibitor, could potentially be used as safe, supportive, and therapeutic product for gout. The extraction of acid from classic Chinese herbal plant Amomum villosum with ethanol was investigated in the study. optimum conditions were determined time 74 min, temperature 48.36 °C, solid-to-liquid ratio 1:35 g·mL-1 using Box-Behnken design (BBD) response surface methodology (RSM). experimental...
Abstract A few studies suggested that CircRNAs were involved in the development of septic AKI. However，the role and regulation mechanism CircRNA_35953 AKI remains unclear. Here, we found Circ_35953 was induced by LPS via activation NF‐κB signal BUMPT cells. Functionally, mediated apoptosis Moreover, demonstrated sponged miR‐7219‐5p to upregulate expression HOOK3 IGFBP7. Finally, verified knock down alleviated progression CLP‐induced targeting miR‐7219‐5p/HOOK3 IGFBP7 signal. Collectively,...
Irinotecan is the first line chemotherapy drug used for treatment of metastatic colorectal cancer worldwide. There increasing evidence suggesting that liver damage, including steatosis and steatohepatitis, can be caused during involving irinotecan. However, molecular mechanisms by which irinotecan-induced injury remain elusive. In this study, we found irinotecan significant elevation ALT, inflammation, fat accumulation in liver, are associated with hepatic macrophage activation. Depletion...
In the heart, protein kinase A (PKA) is critical for activating calcium handling and sarcomeric proteins in response to beta-adrenergic stimulation leading increased myocardial contractility performance. The catalytic activity of PKA tightly regulated by regulatory subunits that inhibit subunit until released cAMP binding. Phosphorylation type II promotes activation; however, role phosphorylation I remain uncertain. Here, we utilize human induced pluripotent stem cell cardiomyocytes...
Small ubiquitin-like modifier specific peptidase 2 (SENP2) suppresses the progression and chemoresistance of several cancers, while few studies report its role in hepatocellular carcinoma (HCC). This study aimed to evaluate effect SENP2 on stemness, sorafenib sensitivity, downstream pathway HCC, with validation molecular mechanisms by compensation experiment.SENP2 was regulated plasmid transfection; meanwhile, a experiment, protein kinase B (AKT) activated SC79 treatment β-catenin (CTNNB1)...
To analyze the differentially expressed exosomal miRNAs in subchondral osteoblasts patients with osteoarthritis (OA) and to investigate key potentially involved occurrence progression of OA. Methods: Subchondral bones were harvested from 6 All subjects divided into two groups which was based on severity joint wear: An OA group, severely worn side bone, a control less bone. The exosomes extracted osteoblast cells their characteristics identified. Then sequencing analysis conducted compare...
It has been previously postulated that blood neurotransmitters might affect risks of neurodegenerative diseases. Here, a Mendelian Randomization (MR) study was conducted to explore whether genetically predicted concentrations glycine, glutamate and serotonin were associated with Alzheimer's disease (AD), Parkinson's (PD), amyotrophic lateral sclerosis (ALS). From three genome-wide association studies European ancestry, single nucleotide polymorphisms strongly selected as genetic instrumental...
Background: The prediction of mortality for septic acute kidney injury (AKI) has been assessed by a number potential biomarkers, including long non-coding RNAs (lncRNAs). However, the validation lncRNAs as particularly early stages AKI, is still warranted.Methods: Human blood and urine samples were collected from healthy volunteers(n =30), sepsis patients with AKI (n =96) without (n=96) LncRNA chip assay, RT-qPCR, absolute real-time PCR analysis. renal tubular epithelial (HK-2) cells C57B6...
Tumor is a new organism formed by abnormal hyperplasia of local tissue cells under the action various tumorigenic factors. Inflammation plays decisive role in inducing tumorigenesis, promoting tumor development, invasion and migration. More more evidence indicate that exosomes are involved regulating formation microenvironment process proinflammatory carcinogenesis, leading to stimulation anti-tumor immune response or systemic immunosuppression, play crucial development tumor.