A5009968705- RNA Research and Splicing
- Tuberculosis Research and Epidemiology
- Neurological Disease Mechanisms and Treatments
- Mycobacterium research and diagnosis
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Biochemical effects in animals
- Viral Infections and Immunology Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Neurogenetic and Muscular Disorders Research
- Medical Research and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Chronic Kidney Disease and Diabetes
- Cancer-related molecular mechanisms research
- Diagnosis and treatment of tuberculosis
- Ferroptosis and cancer prognosis
- Genetic Associations and Epidemiology
- Wnt/β-catenin signaling in development and cancer
- Kidney Stones and Urolithiasis Treatments
- Neonatal and fetal brain pathology
- RNA modifications and cancer
- Renal Diseases and Glomerulopathies
Shanghai University of Traditional Chinese Medicine
2023-2024
Yueyang Hospital
2023-2024
Second Xiangya Hospital of Central South University
2020
Central South University
2020
Fudan University
2007-2018
Shanghai Medical College of Fudan University
2014-2015
State Key Laboratory of Medical Neurobiology
2007
Recent studies have reported that upregulation of disulfide-bond A oxidoreductase-like protein (DsbA-L) prevented lipid-induced renal injury in diabetic nephropathy (DN). However, the role and regulation proximal tubular DsbA-L for tubulointerstitial fibrosis (TIF) remains unclear. In current study, we found a tubules-specific knockout mouse (PT-DsbA-L-KO) attenuated UUO-induced TIF, cell apoptosis inflammation. Mechanistically, interacted with Hsp90 mitochondria BUMPT cells which activated...
Mycobacterium tuberculosis (M. tb) infection is initiated by the few bacilli inhaled into alveolus. Studies in lungs of aerosol-infected mice provided evidence for extensive replication M. tb non-migrating, non-antigen-presenting cells alveoli during first 2–3 weeks post-infection. Alveoli are lined type II and I alveolar epithelial (AEC) which outnumber macrophages several hundred-fold. DNA viable have been demonstrated AEC other non-macrophage kidney, liver, spleen autopsied tissues from...
To explore the impact of inflammatory factors on incidence cerebral small vessel disease (CSVD), we performed a mendelian randomization (MR) study to analyze causal relationship between multiple and CSVD imaging markers utilized summary-data-based (SMR) analysis infer whether instrumental variables (IVs) is mediated by gene expression or DNA methylation.
Hematogenous dissemination of Mycobacterium tuberculosis (M. tb) occurs during both primary and reactivated (TB). Although hematogenous in non-HIV TB patients, ∼80% these manifests exclusively as pulmonary disease. In contrast, extrapulmonary, disseminated, and/or miliary is seen 60–70% HIV-infected suggesting that likely more common HIV+ patients. To understand M. tb adaptation to the blood environment bacteremia, we have studied transcriptome replicating human whole blood. investigate if...
Huzhangqingmaiyin (HZQMY) is a Chinese medicine formula used to treat small vessel disease, but the mechanism unclear.This study aimed reveal protective effects of HZQMY on human brain microvascular endothelial cells (HBMECs) and explore potential targets mechanistic pathways using network pharmacology treating cerebral disease (CSVD).HBMECs were cultured in vitro an cell injury model was constructed by hypoxia for 12 h followed reoxygenation 8 (H/R). Cell viability measured CCK-8 assay,...
The objective of this study was to evaluate the expression genes encoding SR proteinsand alternative splicing IL4 and TLR4 in Mycobacterium tuberculosis (M. tb) H37Rv-infected macrophages.THP-1 cells were induced differentiate into macrophages with 200 nM PMA, H37Rv strains used for macrophage infection. After RNA extraction, qRT-PCR performed many proteins as well TLR4.IL4 play significant roles host immunity tuberculosis. level IL-4 splice variants THP-1 increased after M. tb Three...
Alveolar epithelial cells outnumber alveolar macrophages by ~500 fold and increasing evidence suggests Mycobacterium tuberculosis may replicate dramatically in these during the initial weeks of infecting lung [1,2]. Here, we report experimental detail transcriptional profiling replicating at 72 hr post-infection human type II cell line, A549, as compared to growing logarithmically laboratory broth culture [2]. All resulting data was deposited Gene Expression Omnibus (GEO) database under...
Background Mycobacterium tuberculosis and HIV act synergistically to enhance accelerate the development of progression infection AIDS. Hematogenous dissemination M. leading extrapulmonary TB, disseminated TB miliary is greatly increased in HIV+ patients. We have compared transcriptome replicating whole blood from immunocompetent immunodeficient individuals understand how adapts environment during hematogenous dissemination.
Neural salient serine/arginine-rich protein 1 (NSSR1) has been found to play important roles in inhibiting alternative splicing during heat shock and mitosis is predominantly expressed neural tissues such as cerebral neurons, cerebellar Purkinje cells bipolar of the retina. Recently, NSSR1 also shown be highly testes, suggesting its potential reproductive system. In this report, expression columnar epithelium endometrium gland development mouse uterus, regulation level by testosterone adult...
Neural salient serine/arginine-rich protein 1 (NSSR1) expression has been found in mouse cerebral neurons, cerebellar Purkinje cells, pyramidal neurons and granule cells of dentate gyrus regulates the pre-mRNA splicing genes important for neural functions. In this study, we demonstrated that NSSR1 is expressed rat retina extensively distributed outer inner plexiform layers. Double staining experiments showed mainly ON-type bipolar localized dendrites, somata axon terminals. The result...
Neural salient serine/arginine-rich protein 1 (NSSR1, alternatively SRp38) is an important splicing factor that can repress pre-mRNA alternative in cells during heat shock and mitosis.We show here NSSR1 dephosphorylated when are shocked or incubated with kinase inhibitor K252a.Both K252a treatment increase the truncated isoform of GluR-B minigene pre-mRNA.We also investigated roles RRM motif three RS domains vivo splicing.The results deletion did not affect splicing, but any one increases...
To investigate the expression of neural salient serine/arginine-rich protein 1 (NSSR1) in development mouse brain.Brain samples were collected from mice with different developmental stages: 9, 12, 14 d before birth (E9, E12, E14) and d, 3 weeks months after birth. The NSSR1 brain at stages was detected by Western blot distribution analyzed immunohistochemical staining. compared among embryos, neonatal adult animals.During embryogenesis, proteins increases significantly 0.186(E9) to...
To establish a minigene model of neural cell adhesion molecule L1 (NCAM L1) gene and to study its splicing patterns in different lines.Using human genetic cDNA as template, the NCAM fragment was amplified inserted into eukaryotic expression vector. The transfected 4 lines these were studied.The individual lines. In PFSK Hela lines, two splicied isoforms generated but COS-1 R28 only one isoform existed.NCAM can be used alternative analysis.