A5083229215- Osteoarthritis Treatment and Mechanisms
- Inflammatory mediators and NSAID effects
- Bone Metabolism and Diseases
- Peroxisome Proliferator-Activated Receptors
- Infrared Thermography in Medicine
- Bone Tissue Engineering Materials
- Metabolomics and Mass Spectrometry Studies
- Cancer-related molecular mechanisms research
- Adipokines, Inflammation, and Metabolic Diseases
- Fatty Acid Research and Health
- Bone and Joint Diseases
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Dental Implant Techniques and Outcomes
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Cytokine Signaling Pathways and Interactions
- Spectroscopy Techniques in Biomedical and Chemical Research
- Orthopaedic implants and arthroplasty
- Bone health and treatments
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Angiogenesis and VEGF in Cancer
Queensland University of Technology
2016-2025
Medical Technologies (Czechia)
2023
The contribution of metabolic factors on the severity osteoarthritis (OA) is not fully appreciated. This study aimed to define effects hypercholesterolemia progression OA. Apolipoprotein E-deficient (ApoE-/-) mice and rats with diet-induced (DIHC) were used explore Both models exhibited OA-like changes, characterized primarily by a loss proteoglycans, collagen aggrecan degradation, osteophyte formation, changes subchondral bone architecture, cartilage degradation. Surgical destabilization...
Abstract The predominant saturated fatty acids (SFA) in human diets are lauric acid (LA, C12:0), myristic (MA, C14:0), palmitic (PA, C16:0) and stearic (SA, C18:0). aim of this study was to investigate whether containing individual SFA together with excess simple carbohydrates induce osteoarthritis (OA)-like changes knee joints signs metabolic syndrome rats. Rats were given either a corn starch diet or composed 20% LA, MA, PA, SA beef tallow for 16 weeks. fed tallow, SA, MA PA developed...
Objectives Epidemiological and experimental studies have established obesity to be an important risk factor for osteoarthritis (OA), however, the mechanisms underlying this link remains largely unknown. Here, we studied local inflammatory responses in metabolic-OA. Methods Wistar rats were fed with control diet (CD) high-carbohydrate, high-fat (HCHF) period of 8 16 weeks. After euthanasia, knees examined assess articular cartilage changes inflammation synovial membrane. Further IHC was...
Abstract Osteoarthritis (OA) is a debilitating degenerative disease affecting multiple joint tissues, including cartilage, bone, synovium, and adipose tissues. OA presents diverse clinical phenotypes distinct molecular endotypes, inflammatory, metabolic, mechanical, genetic, synovial variants. Consequently, innovative technologies are needed to support the development of effective diagnostic precision therapeutic approaches. Traditional analysis bulk tissue extracts has limitations due...
Abstract Objective Previous studies have shown the influence of subchondral bone osteoblasts (SBOs) on phenotypical changes articular cartilage chondrocytes (ACCs) during development osteoarthritis (OA). The molecular mechanisms involved this process remain elusive, in particular, signal transduction pathways. aim study was to investigate vitro effects OA SBOs normal ACCs and unveil potential involvement MAPK signaling pathways process. Methods Normal arthritic samples were collected for...
Subchondral bone sclerosis is a well-recognised manifestation of osteoarthritis (OA).The osteocyte cell network now considered to be central the regulation homeostasis; however, it not known whether integrity altered in OA patients.The aim this study was investigate phenotypic changes and its potential role subchondral pathogenesis.The morphological osteocytes samples were investigated by micro-CT, SEM, histology, immunohistochemistry, TRAP staining, apoptosis assay real-time PCR studies.We...
Objective. Degradative enzymes, such as A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) matrix metalloproteinases (MMP), play key roles in development of osteoarthritis (OA). We investigated if crosstalk between subchondral bone osteoblasts (SBO) articular cartilage chondrocytes (ACC) OA alters the expression regulation ADAMTS5, ADAMTS4, MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-13, also tested possible involvement mitogen-activated protein kinase (MAPK) signaling...
Abstract Non-resolved persistent macrophage-mediated synovial inflammation is considered as one of the main drivers both establishment and progression obesity-associated osteoarthritis (OA). Herein, we used clodronate-loaded liposomes (CL) to locally deplete macrophages in joints examine role obesity-induced OA. Furthermore, resolvin D1 (RvD1), a unique family pro-resolving lipid mediator derived from omega-3 polyunsaturated fatty acid, have shown marked potency changing pro-inflammatory...
Altered subchondral bone and articular cartilage interactions have been implicated in the pathogenesis of osteoarthritis (OA); however, mechanisms remain unknown. Exosomes are membrane-derived vesicles that recently recognized as important mediators intercellular communication. Herein, we investigated if OA derived exosomes alter transcriptional bioenergetic signatures chondrocytes. were isolated purified from osteoblasts nonsclerotic or sclerotic zones human their role on chondrocytes was...
Articular cartilage function depends on the temporal and zonal distribution of coordinated metabolic regulation in chondrocytes. Emerging evidence shows importance cellular metabolism molecular control its dysregulation degenerative diseases like osteoarthritis (OA). Compared to most other tissues, chondrocytes are sparsely located extracellular matrix, lacking typical proximity neural, vascular, lymphatic tissue. Making up under 5% total tissue weight cartilage, have a relative deficiency...
Abstract Osteoarthritis (OA) is characterized by the dysregulation of osteochondral interface between bone and cartilage. In vitro, models are crucial for studying OA testing treatments. However, current limited to replicating extracellular matrix's structural mechanical heterogeneity do not account distinct oxygen gradients that chondrocytes osteoblasts experience at interface. By using micropatterned granular hydrogels control scavenging agents' delivery, maintaining <1% concentration...
Review Insights into Bioengineering Approaches for Aging Bone Regeneration: Strategies to Target Osteoimmunosenescence Lan Xiao 1,2,†, Wendong Gao Jinfu Wu 3, Itsasne Erezuma 4, Alireza Dolatshahi-Pirouz 5, Joana Silva-Correia 6,7, Yinghong Zhou 2,8, Antonia Rujia Sun 2,9, Indira Prasadam Ross Crawford Joaquim Miguel Oliveira Gorka Orive 5,10,11,12,13, Chengtie 3 and Yin 1,2,* 1 School of Medicine Dentistry, Griffith University (GU), Gold Coast, QLD 4222, Australia 2 The Australia-China...
Aging reduces NAD + levels, affecting metabolism. Traditional studies are destructive, limiting tracking. We present a non-invasive optical method with NMN-coated microneedles and multiphoton microscopy to monitor shifts in real-time, validated keratinocytes mouse skin.