A5044923140- Circadian rhythm and melatonin
- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Insect and Arachnid Ecology and Behavior
- Light effects on plants
- Insect Pheromone Research and Control
- Plant and animal studies
- Spaceflight effects on biology
- Insect and Pesticide Research
- Sperm and Testicular Function
- Animal Behavior and Reproduction
- Dietary Effects on Health
- Psychological and Temporal Perspectives Research
- Insect Utilization and Effects
- Photoreceptor and optogenetics research
- Cholinesterase and Neurodegenerative Diseases
- Insect behavior and control techniques
- Plant Molecular Biology Research
- Insect Resistance and Genetics
- Alzheimer's disease research and treatments
- Entomological Studies and Ecology
- Seed Germination and Physiology
- Physiological and biochemical adaptations
- MicroRNA in disease regulation
- bioluminescence and chemiluminescence research
Oregon State University
2012-2022
University of Massachusetts Chan Medical School
2006
University of Regensburg
2002
United States Department of Agriculture
1994
Agricultural Research Service
1988-1994
University of Maryland, College Park
1988-1994
Beltsville Agricultural Research Center
1990-1991
Bhabha Atomic Research Centre
1991
The Ohio State University
1985-1986
University of Washington
1984-1985
Although it is well established that the circadian clock regulates mammalian reproductive physiology, molecular mechanisms by which this regulation occurs are not clear. The authors investigated capacity of mice lacking Bmal1 ( Arntl, Mop3), one central genes. They found both male and female knockout (KO) infertile. Gross microscopic inspection anatomy sexes suggested deficiencies in steroidogenesis. Male KO had low testosterone high luteinizing hormone serum concentrations, suggesting a...
Circadian coordination of life functions is believed to contribute an organism's fitness; however, such contributions have not been convincingly demonstrated in any animal. The most significant measure fitness the reproductive output individual and species. Here we examined consequences loss clock function on Drosophila melanogaster with mutated period ( per 0 ), timeless tim cycle cyc Clock Clk Jrk ) genes. Single mating among couples clock-deficient phenotypes resulted ≈40% fewer progeny...
There is increasing evidence that aging affected by biological (circadian) clocks - the internal mechanisms coordinate daily changes in gene expression, physiological functions and behavior with external day/night cycles. Recent data suggest disruption of mammalian circadian clock results accelerated increased age-related pathologies such as cancer; however, links between loss rhythms are not understood. We sought to determine whether affects lifespan healthspan model organism Drosophila...
Abstract Disruption of the circadian clock, which directs rhythmic expression numerous output genes, accelerates aging. To enquire how system protects aging organisms, here we compare transcriptomes in heads young and old Drosophila melanogaster . The core clock most genes remained robustly flies, while others lost rhythmicity with age, resulting constitutive over- or under-expression. Unexpectedly, identify a subset that adopted increased de novo during aging, enriched for stress-response...
Light is necessary for life, but prolonged exposure to artificial light a matter of increasing health concern. Humans are exposed increased amounts in the blue spectrum produced by light-emitting diodes (LEDs), which can interfere with normal sleep cycles. The LED technologies relatively new; therefore, long-term effects across lifespan not understood. We investigated model organism, Drosophila melanogaster, and determined that flies maintained daily cycles 12-h darkness had significantly...
In Drosophila melanogaster, disruption of night by even short light exposures results in degradation the clock protein TIMELESS (TIM), leading to shifts fly molecular and behavioral rhythms. Several lines evidence indicate that entrainment brain involves blue-light photoreceptor cryptochrome (CRY). cryptochrome-depleted ( cry b ), pulses is impaired but still entrainable light-dark cycles, probably due input from visual system. Whether transduction pathways play a role noninnervated,...
Circadian clocks maintain temporal homeostasis by generating daily output rhythms in molecular, cellular, and physiological functions. Output rhythms, such as sleep/wake cycles hormonal fluctuations, tend to deteriorate during aging humans, rodents, fruit flies. However, it is not clear whether this decay caused defects the core transcriptional clock, or weakening of clock-output pathways, both. The authors monitored age-related changes behavioral molecular Drosophila melanogaster. Aging was...
Circadian clocks generate daily rhythms in neuronal, physiological, and metabolic functions. Previous studies mammals reported fluctuations levels of the major endogenous antioxidant, glutathione (GSH), but molecular mechanisms that govern such remained unknown. To address this question, we used model species Drosophila, which has a rich arsenal genetic tools. Previously, showed loss circadian clock increased oxidative damage caused neurodegenerative changes brain, while enhanced GSH...
Lactate dehydrogenase (LDH) catalyzes the conversion of glycolysis-derived pyruvate to lactate.Lactate has been shown play key roles in brain energetics and memory formation.However, lactate levels are elevated aging Alzheimer's disease patients, it is not clear whether plays protective or detrimental these contexts.Here we show that Ldh transcript cycle with diurnal rhythm heads aged flies this associated increased LDH protein, enzyme activity, concentrations.To understand biological...
Circadian clock genes are ubiquitously expressed in the nervous system and peripheral tissues of complex animals. While brain essential for behavioral rhythms, physiological roles these periphery not well understood. Constitutive expression gene period was reported ovaries Drosophila melanogaster; however, its molecular interactions functional significance remained unknown. This study demonstrates that period( per) timeless( tim) involved a novel noncircadian function ovary. PER TIM...
The protein product (PER) of the Drosophila clock gene, period ( per), is involved in a molecular feedback loop which PER inhibits transcription its own mRNA. This causes to cycle circadian manner, and this cycling specific regions brain (the presumed location central pacemaker) responsible for rhythmicity locomotor activity possibly eclosion. has also been detected several nonneural tissues abdomen, but whether exhibits free-running light-sensitive cycles any these not known. In study,...
Background Circadian clocks govern daily physiological and molecular rhythms, putative rhythms in expression of xenobiotic metabolizing (XM) genes have been described both insects mammals. Such could important consequences for outcomes chemical exposures at different times day. To determine whether reported XM gene result functional we examined profiles enzyme activity dose responses to the pesticides propoxur, deltamethrin, fipronil, malathion. Methodology/Principal Findings Published...
Circadian clocks generate daily rhythms in molecular, cellular, and physiological functions providing temporal dimension to organismal homeostasis. Recent evidence suggests two-way relationship between circadian aging. While disruption of the clock leads premature aging animals, there is also age-related dampening output such as sleep/wake cycles hormonal fluctuations. Decay oscillations several genes was recently reported aged fruit flies, but mechanisms underlying these changes are not...
Blue light is a predominant component of emitting devices (LEDs), which are increasingly present in our environment. There already accumulating evidence that blue exposure causes damage to retinal cells vitro and vivo; however, much less known about potential effects on non-retinal cells. That may be detrimental at the organismal level independent from effect was recently shown by findings it reduces lifespan worms also flies with genetically ablated retinas. Here, we investigated across fly...