The innate immune response tends to become hyperactive and proinflammatory in older organisms. We investigated connections between activity of the immune-related genes aging using Drosophila model. A hallmark immunity is production antimicrobial peptides (AMP), whose expression triggered via activation Toll Imd pathways regulated by NF-ĸB-like transcription factors, Dif/Dorsal Relish. It was previously shown that overexpression upstream component shortens lifespan Relish-dependent response....

The hypothesis that overexpression of glutamate-cysteine ligase (GCL), which catalyzes the rate-limiting reaction in de novo glutathione biosynthesis, could extend life span was tested fruit fly, Drosophila melanogaster. GAL4-UAS binary transgenic system used to generate flies overexpressing either catalytic (GCLc) or modulatory (GCLm) subunit this enzyme, a global neuronally targeted pattern. GCL protein content central nervous elevated dramatically presence neuronal drivers. activity...

The redox state of tissues tends to become progressively more prooxidizing during the aging process. hypothesis tested in this study was that enhancement reductive capacity by overexpression glucose-6-phosphate dehydrogenase (G6PD), a key enzyme for NADPH biosynthesis, could protect against oxidative stress and extend life span transgenic Drosophila melanogaster. Overexpression G6PD achieved combining UAS-G6PD responder transgene at one four independent loci with either broad expression...

Peroxiredoxin 5 is a distinct isoform of the peroxiredoxin gene family. The antioxidative and anti-apoptotic functions have been extensively demonstrated in cell culture experiments. In present paper, we provide first functional analysis multicellular organism, Drosophila melanogaster. Similar to its mammalian, yeast or human counterparts, dPrx5 (Drosophila 5) expressed several cellular compartments, including cytosol, nucleus mitochondrion. Global overexpression flies increased resistance...

Circadian clocks generate daily rhythms in neuronal, physiological, and metabolic functions. Previous studies mammals reported fluctuations levels of the major endogenous antioxidant, glutathione (GSH), but molecular mechanisms that govern such remained unknown. To address this question, we used model species Drosophila, which has a rich arsenal genetic tools. Previously, showed loss circadian clock increased oxidative damage caused neurodegenerative changes brain, while enhanced GSH...

Recently, we identified a set of five genes constituting the peroxiredoxin gene family in Drosophila melanogaster [Radyuk, Klichko, Spinola, Sohal and Orr (2001) Free Radical Biol. Med. 31, 1090-1100]. This includes two abundant thioredoxin peroxidase (TPx) species, namely DPx-4783, cytosolic TPx DPx-5037, mitochondrial TPx. Overexpression either one them S2 cells conferred increased resistance to toxicity induced by hydrogen peroxide, paraquat or cadmium. To understand further functional...

Peroxiredoxin 4 (Prx4) has been implicated in a wide variety of biological processes, including development, progression cancer, inflammation, and antioxidant function. The purpose this study was to provide further insight into its multiple roles at the whole-animal level, using Drosophila. Reduced expression dPrx4 (up 90%) resulted greater sensitivity oxidative stress, an elevated H₂O₂ flux, increases lipid peroxidation, but no effect on longevity. Overexpression low levels (<2-fold) gave...

A subset of patients suffering from familial amyotrophic lateral sclerosis (FALS) exhibit point mutations in the gene encoding Cu-Zn superoxide dismutase [superoxide:superoxide oxidoreductase, EC (SOD)]. The human wild-type and five FALS Sod mutant transgenes were introduced into fruit fly, Drosophila melanogaster, a null background. flies had dramatically decreased life span, glutathione methionine content, fertility, locomotor activity, resistance to hyperoxic stress, compared with...

Circadian coordination of metabolism, physiology, and neural functions contributes to healthy aging disease prevention. Clock genes govern the daily rhythmic expression target whose activities underlie such broad physiological parameters as maintenance redox homeostasis. Previously, we reported that glutathione (GSH) biosynthesis is controlled by circadian system via effects clock on catalytic (Gclc) modulatory (Gclm) subunits comprising glutamate cysteine ligase (GCL) holoenzyme. The...

Abstract Catalase represents one of the key antioxidant enzymes (AOE) in metabolism oxygen free radicals. A comprehensive analysis was brought to bear on establishing catalase gene expression profiles during development and aging, with underlying objective being identify potential regulatory factors. Expression exhibits substantial variations aging a stage‐ tissue‐specific manner. At temporal level, previous observations coincidence ecdysteroid pulses peaks developmental stages were largely...

In the present study, we have examined catalase protein and mRNA levels factors that may regulate expression in Drosophila melanogaster during development. Both changes are general accord with variations ecdysteroid titer Differences accumulation profiles, particularly embryos young adults, suggest be regulated at both transcriptional post-transcriptional levels. It was possible to induce by administering exogenous 20-hydroxyecdysone (Ec) culture certain stages of development (usually time...

A notable extension of life span (up to 50%) was achieved in Drosophila melanogaster when the catalytic subunit glutamate-cysteine ligase (GCLc) overexpressed neuronal tissue, while a moderate increase 24%) observed modulatory GCL (GCLm) globally. We sought identify specific tissue domains that are particularly sensitive beneficial effects GCLc overexpression. Overexpression using mushroom body driver (OK107-GAL4) had small but significant effect on longevity (approximately 12%)...

Mitochondrial dysfunction often leads to neurodegeneration and is considered one of the main causes neurological disorders, such as Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS) other age-related diseases. tightly linked oxidative stress accumulating evidence suggests association between disorders. However, there insufficient knowledge about role pro-oxidative shift in cellular redox impairment redox-sensitive signaling development neurodegenerative pathological conditions....