The advent of inexpensive digital image sensors and the ability to create photographs that combine information from a number sensed images are changing way we think about photography. In this paper, describe unique array 100 custom video cameras have built, summarize our experiences using in range imaging applications. Our goal was explore capabilities system would be produce future. With mind, used simple cameras, lenses, mountings, assumed processing large numbers eventually easy cheap....

Image blur is caused by a number of factors such as motion, defocus, capturing light over the non-zero area aperture and pixel, presence anti-aliasing filters on camera sensor, limited sensor resolution. We present an algorithm that estimates non-parametric, spatially-varying functions (i.e., point-spread or PSFs) at subpixel resolution from single image. Our method handles due to slight inherent aspects imaging system. can be used measure estimating sub-pixel, super-resolved PSF even for...

One hit wonder: A biomimetic transamination reaction has been developed that employs pyridoxal-5-phosphate to modify the N terminus of proteins and peptides under mild conditions. This technique introduces a uniquely reactive carbonyl group in single location (see scheme), thus allowing further elaboration through oxime or hydrazone formation. modification strategy is also compatible with containing free cysteine residue.

Inorganic-biological hybrid systems have potential to be sustainable, efficient, and versatile chemical synthesis platforms by integrating the light-harvesting properties of semiconductors with synthetic biological cells. We developed a modular bioinorganic platform that consists highly efficient indium phosphide nanoparticles genetically engineered Saccharomyces cerevisiae, workhorse microorganism in biomanufacturing. The yeast harvests photogenerated electrons from illuminated uses them...

Abstract Mucosal healing plays a critical role in combatting the effects of inflammatory bowel disease, fistulae and ulcers. While most treatments for such diseases focus on systemically delivered anti-inflammatory drugs, often leading to detrimental side effects, mucosal agents that target gut epithelium are underexplored. We genetically engineer Escherichia coli Nissle 1917 (EcN) create fibrous matrices promote epithelial integrity situ. These consist curli nanofibers displaying trefoil...

We present a deblurring algorithm that uses hardware attachment coupled with natural image prior to deblur images from consumer cameras. Our approach combination of inexpensive gyroscopes and accelerometers in an energy optimization framework estimate blur function the camera's acceleration angular velocity during exposure. solve for camera motion at high sampling rate exposure infer latent using joint optimization. method is completely automatic, handles per-pixel, spatially-varying blur,...

It is often desirable to evaluate an image based on its quality. For many computer vision applications, a perceptually meaningful measure the most relevant for evaluation; however, commonly used do not map well human judgements of A further complication existing that they require reference image, which available in practice. In this paper, we present "blind" quality measure, where potentially neither groundtruth nor degradation process are known. Our method uses set novel low-level features...

Abstract A typical camera trap survey may produce millions of images that require slow, expensive manual review. Consequently, critical conservation questions be answered too slowly to support decision‐making. Recent studies demonstrated the potential for computer vision dramatically increase efficiency in image‐based biodiversity surveys; however, literature has focused on projects with a large set labelled training images, and hence many smaller cannot benefit from existing machine...

The gut microbiota represents a large community of microorganisms that play an important role in immune regulation and maintenance homeostasis. Living bacteria receive increasing interest as potential therapeutics for disorders, because they inhibit the colonization pathogens positively regulate composition gut. However, these treatments are often accompanied by antibiotic administration targeting pathogens. In cases, efficacy therapeutic is compromised their susceptibility to antibiotics....

A new selective bioconjugation reaction is described for the modification of tyrosine residues on protein substrates. The uses imines formed in situ from aldehydes and electron-rich anilines to modify phenolic side chains through a Mannich-type electrophilic aromatic substitution pathway. takes place under mild pH temperature conditions can substrates at concentrations as low 20 μM. Using an efficient fluorescence-based assay, we demonstrated using number targets. Importantly, proteins...

The advent of inexpensive digital image sensors and the ability to create photographs that combine information from a number sensed images are changing way we think about photography. In this paper, describe unique array 100 custom video cameras have built, summarize our experiences using in range imaging applications. Our goal was explore capabilities system would be produce future. With mind, used simple cameras, lenses, mountings, assumed processing large numbers eventually easy cheap....

A light field consists of images a scene taken from different viewpoints. Light fields are used in computer graphics for image-based rendering and synthetic aperture photography, vision recovering shape. In this paper, we describe simple procedure to calibrate camera arrays capture using plane + parallax framework. Specifically, the case when cameras lie on plane, show (i) how estimate positions up an affine ambiguity, (ii) reproject onto family planes only knowledge planar one point scene....

Image blur and noise are difficult to avoid in many situations can often ruin a photograph. We present novel image deconvolution algorithm that deblurs denoises an given known shift-invariant kernel. Our uses local color statistics derived from the as constraint unified framework be used for deblurring, denoising, upsampling. A pixel's is required linear combination of two most prevalent colors within neighborhood pixel. This two-color prior has major benefits: it tuned content particular...

The restoration of a blurry or noisy image is commonly performed with MAP estimator, which maximizes posterior probability to reconstruct clean from degraded image. A when used sparse gradient prior, reconstructs piecewise smooth images and typically removes textures that are important for visual realism. We present an alternative deconvolution method called iterative distribution reweighting (IDR) imposes global constraint on gradients so reconstructed should have similar reference...

Injectable gelatin hydrogels formed with bioorthogonal click chemistry (ClickGel) are cell-responsive ECM mimics for in vitro and vivo biomaterials applications. Gelatin polymers pendant norbornene (GelN) or tetrazine (GelT) groups can quickly spontaneously crosslink upon mixing, allowing high viability of encapsulated cells, establishment 3D elongated cell morphologies, biodegradation when injected vivo. As a service to our authors readers, this journal provides supporting information...

Drones equipped with cameras are emerging as a powerful tool for large-scale aerial 3D scanning, but existing automatic flight planners do not exploit all available information about the scene, and can therefore produce inaccurate incomplete models. We present an method to generate drone trajectories, such that imagery acquired during will later high-fidelity model. Our uses coarse estimate of scene geometry plan camera trajectories that: (1) cover thoroughly possible; (2) encourage...

ABSTRACT Biocatalytic transformations generally rely on purified enzymes or whole cells to perform complex that are used industrial scale for chemical, drug, and biofuel synthesis, pesticide decontamination, water purification. However, both of these systems have inherent disadvantages related the costs associated with enzyme purification, long‐term stability immobilized enzymes, catalyst recovery, compatibility harsh reaction conditions. We developed a novel strategy producing rationally...

Abstract A notable challenge for the design of engineered living materials (ELMs) is programming a cellular system to assimilate resources from its surroundings and convert them into macroscopic with specific functions. Here, an ELM that uses Escherichia coli as chassis curli nanofibers extracellular matrix component demonstrated. Cell‐laden hydrogels are created by concentrating curli‐producing cultures. The rheological properties modulated genetically encoded factors processing steps. have...

Synthetic biology approaches to bioremediation are a key sustainable strategy leverage the self-replicating and programmable aspects of for environmental stewardship. The increasing spread anthropogenic mercury pollution into our habitats food chains is pressing concern. Here, we explore use programmed bacterial biofilms aid in sequestration mercury. We demonstrate that by integrating mercury-responsive promoter an operon encoding mercury-absorbing self-assembling extracellular protein...